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A2SHT9

- MDH_METPP

UniProt

A2SHT9 - MDH_METPP

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Protein

Malate dehydrogenase

Gene

mdh

Organism
Methylibium petroleiphilum (strain PM1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951SubstrateUniRule annotation
Binding sitei101 – 1011SubstrateUniRule annotation
Binding sitei108 – 1081NADUniRule annotation
Binding sitei115 – 1151NADUniRule annotation
Binding sitei134 – 1341SubstrateUniRule annotation
Binding sitei165 – 1651SubstrateUniRule annotation
Active sitei190 – 1901Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NADUniRule annotation
Nucleotide bindingi132 – 1343NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMPET420662:GHBE-2201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Mpe_A2172
OrganismiMethylibium petroleiphilum (strain PM1)
Taxonomic identifieri420662 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesMethylibium
ProteomesiUP000000366: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 328327Malate dehydrogenasePRO_0000294391Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi420662.Mpe_A2172.

Structurei

3D structure databases

ProteinModelPortaliA2SHT9.
SMRiA2SHT9. Positions 3-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFSQECI.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2SHT9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKKPVRVAV TGAAGQIGYA LLFRIASGEM LGKDQPVILQ LLEIPDEKAQ
60 70 80 90 100
KALKGVMMEL EDCAFPLLAG MEAHGDPMTA FKDADYALLV GSRPRGPGME
110 120 130 140 150
RAELLSINGA IFTAQGKALN AVASRNVKVL VVGNPANTNA YIAMKAAPDL
160 170 180 190 200
PRKNFTAMLR LDHNRAASQI AAKTGKPVSS IKQLAVWGNH SPTMYADYRF
210 220 230 240 250
ATIDGASVKD MINDQVWNKD VFLPTVGKRG AAIIEARGLS SAASAANAAI
260 270 280 290 300
DHMRDWALGT NGAWVTMGVP SNGEYGIPKD VMFGFPVTCA NGEYKIVDGL
310 320
AIDAFSQECI NKTLAELQGE QDGVKHLI
Length:328
Mass (Da):34,943
Last modified:March 6, 2007 - v1
Checksum:i66D86E09AA1F2E4B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000555 Genomic DNA. Translation: ABM95128.1.
RefSeqiWP_011829765.1. NC_008825.1.
YP_001021363.1. NC_008825.1.

Genome annotation databases

EnsemblBacteriaiABM95128; ABM95128; Mpe_A2172.
GeneIDi4784861.
KEGGimpt:Mpe_A2172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000555 Genomic DNA. Translation: ABM95128.1 .
RefSeqi WP_011829765.1. NC_008825.1.
YP_001021363.1. NC_008825.1.

3D structure databases

ProteinModelPortali A2SHT9.
SMRi A2SHT9. Positions 3-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 420662.Mpe_A2172.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM95128 ; ABM95128 ; Mpe_A2172 .
GeneIDi 4784861.
KEGGi mpt:Mpe_A2172.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000220953.
KOi K00024.
OMAi AFSQECI.
OrthoDBi EOG6PP9Q2.

Enzyme and pathway databases

BioCyci MPET420662:GHBE-2201-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1."
    Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R.
    J. Bacteriol. 189:1931-1945(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PM1.

Entry informationi

Entry nameiMDH_METPP
AccessioniPrimary (citable) accession number: A2SHT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 6, 2007
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3