A2SH88 (A2SH88_METPP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523
EC=2.3.1.- HAMAP MF_00523

Gene names

Name:	lpxD HAMAP MF_00523
Ordered Locus Names:	Mpe_A1969

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP] EMBL ABM94927.1

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Methylibium

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523 UDP-3-O-(3-hydroxytetradecanoyl)glucosamine + (R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] = UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + [acyl-carrier-protein]. SAAS SAAS020573
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523 Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 3/6. SAAS SAAS020573
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily. HAMAP MF_00523

Ontologies

Keywords
Biological process	Lipid A biosynthesis HAMAP MF_00523 SAAS SAAS020573 Lipid synthesis
Domain	Repeat HAMAP MF_00523 SAAS SAAS020573
Molecular function	Acyltransferase HAMAP MF_00523 SAAS SAAS020573 EMBL ABM94927.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A2SH88 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: C190DF648D2FE32C	FASTA	365	37,353
	10 20 30 40 50 60 MIDSAEVTLG DIAAALGGEL IGPKSLDIAR IGPLEDADAA TISFLANPRY RAQLAGSSAG 70 80 90 100 110 120 CVIVAPALRD EAVARGAAIV TPDPYLYFAR LTQWWARRVR PAAVTGVHPS AVVDPGARLG 130 140 150 160 170 180 AGVSIGALAV VEAGAQIDDG AEIGAQCFVG RAARVGAGTR LHPRVTLAFE CVVGARCIVH 190 200 210 220 230 240 SGAVIGADGF GFAPSREHGY VKIEQLGAVR IGDDVEIGAN TCIDRGALGD TVIEDGVKLD 250 260 270 280 290 300 NLIQIAHNVR IGRGTAMAAF TGVAGSTRIG EGCTFGGGAG VVGHVSIADG VHVSAHTAVL 310 320 330 340 350 360 RSIDKPGVYT GIFPLAENRA WEKTAVTLRQ LPALRERIRA LERQAAAASD QEPGAADANG AENTP

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed: 17158667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000555 Genomic DNA. Translation: ABM94927.1.
RefSeq	YP_001021162.1. NC_008825.1.
3D structure databases
ProteinModelPortal	A2SH88.
ModBase	Search...
Protein-protein interaction databases
STRING	A2SH88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4784755.
GenomeReviews	Gene locus Mpe_A1969 in contig CP000555_GR.
KEGG	mpt:Mpe_A1969.
NMPDR	fig|279263.3.peg.1052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	A2SH88.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A2SH88_METPP

Accession

Primary (citable) accession number: A2SH88

Entry history

Integrated into UniProtKB/TrEMBL:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)