A2SH88 (A2SH88_METPP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase HAMAP-Rule MF_00523
EC=2.3.1.- HAMAP-Rule MF_00523

Gene names

Name:	lpxD HAMAP-Rule MF_00523
Ordered Locus Names:	Mpe_A1969 EMBL ABM94927.1

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP] EMBL ABM94927.1

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › unclassified Burkholderiales › Burkholderiales Genera incertae sedis › Methylibium › Methylibium petroleiphilum

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP-Rule MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP-Rule MF_00523
Subunit structure	Homotrimer By similarity. HAMAP-Rule MF_00523 SAAS SAAS007691
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily. HAMAP-Rule MF_00523

Ontologies

Keywords
Biological process	Lipid A biosynthesis HAMAP-Rule MF_00523 SAAS SAAS007691 Lipid biosynthesis Lipid metabolism
Domain	Repeat HAMAP-Rule MF_00523 SAAS SAAS007691
Molecular function	Acyltransferase HAMAP-Rule MF_00523 SAAS SAAS007691 EMBL ABM94927.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	247	1	Proton acceptor By similarity HAMAP-Rule MF_00523

Sequences

	Sequence		Length	Mass (Da)
	A2SH88 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: C190DF648D2FE32C	FASTA	365	37,353
	10 20 30 40 50 60 MIDSAEVTLG DIAAALGGEL IGPKSLDIAR IGPLEDADAA TISFLANPRY RAQLAGSSAG 70 80 90 100 110 120 CVIVAPALRD EAVARGAAIV TPDPYLYFAR LTQWWARRVR PAAVTGVHPS AVVDPGARLG 130 140 150 160 170 180 AGVSIGALAV VEAGAQIDDG AEIGAQCFVG RAARVGAGTR LHPRVTLAFE CVVGARCIVH 190 200 210 220 230 240 SGAVIGADGF GFAPSREHGY VKIEQLGAVR IGDDVEIGAN TCIDRGALGD TVIEDGVKLD 250 260 270 280 290 300 NLIQIAHNVR IGRGTAMAAF TGVAGSTRIG EGCTFGGGAG VVGHVSIADG VHVSAHTAVL 310 320 330 340 350 360 RSIDKPGVYT GIFPLAENRA WEKTAVTLRQ LPALRERIRA LERQAAAASD QEPGAADANG AENTP

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PM1 EMBL ABM94927.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000555 Genomic DNA. Translation: ABM94927.1.
RefSeq	YP_001021162.1. NC_008825.1.
3D structure databases
ProteinModelPortal	A2SH88.
ModBase	Search...
Protein-protein interaction databases
STRING	420662.Mpe_A1969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABM94927; ABM94927; Mpe_A1969.
GeneID	4784755.
KEGG	mpt:Mpe_A1969.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HOG000294339.
KO	K02536.
OMA	SHAFKIP.
Enzyme and pathway databases
BioCyc	MPET420662:GHBE-2685-MONOMER.
UniPathway	UPA00973.
Family and domain databases
HAMAP	MF_00523. LpxD.
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A2SH88_METPP

Accession

Primary (citable) accession number: A2SH88

Entry history

Integrated into UniProtKB/TrEMBL:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)