A2SEX7 (PANC_METPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Mpe_A1155

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › unclassified Burkholderiales › Burkholderiales Genera incertae sedis › Methylibium › Methylibium petroleiphilum

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP-Rule MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP-Rule MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP-Rule MF_00158
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP-Rule MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Pantothenate synthetase HAMAP-Rule MF_00158		PRO_0000305482
Regions
	Nucleotide binding	26 – 33	8	ATP By similarity
	Nucleotide binding	144 – 147	4	ATP By similarity
	Nucleotide binding	181 – 184	4	ATP By similarity
Sites
	Active site	33	1	Proton donor By similarity
	Binding site	57	1	Beta-alanine By similarity
	Binding site	57	1	Pantoate By similarity
	Binding site	150	1	Pantoate By similarity
	Binding site	173	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SEX7 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 9894E4C62FD9D703	FASTA	281	30,514
	10 20 30 40 50 60 MRVVHTINEL RQALAATRPA ALVPTMGSLH EGHLALVKQA RDHGGPVVAS IFVNRLQFAP 70 80 90 100 110 120 HEDFDRYPRT LERDCDLLKG VGCNLVFAPT ESELYPEPQT YKVQPPGELA DVLEGAFRPG 130 140 150 160 170 180 FFTGVCTVVM KLFQCVQPRV AVFGKKDYQQ LMVLRGMVRQ FALPIEIVAV DTARADDGLA 190 200 210 220 230 240 LSSRNAYLSE PERAEAPQLA ATLHTVQAAV LAAVKSGARI DAAALEAQAM GTLRSRGWAP 250 260 270 280 DYVALRRSSD LQAPADPTAE PLVVLAAARL GKTRLIDNLE I

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000555 Genomic DNA. Translation: ABM94116.1.
RefSeq	YP_001020351.1. NC_008825.1.
3D structure databases
ProteinModelPortal	A2SEX7.
ModBase	Search...
Protein-protein interaction databases
STRING	420662.Mpe_A1155.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABM94116; ABM94116; Mpe_A1155.
GeneID	4785730.
KEGG	mpt:Mpe_A1155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HOG000175516.
KO	K01918.
OMA	HTIVSVF.
Enzyme and pathway databases
BioCyc	MPET420662:GHBE-1870-MONOMER.
UniPathway	UPA00028; UER00005.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00158. PanC.
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_METPP

Accession

Primary (citable) accession number: A2SEX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program