Reviewed, UniProtKB/Swiss-Prot A2SDV6 (PANB1_METPP)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase 1
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase 1
      Short name=KPHMT 1

Gene names

Name:	panB1
Ordered Locus Names:	Mpe_A0783

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Methylibium

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	3-methyl-2-oxobutanoate hydroxymethyltransferase 1 HAMAP MF_00156		PRO_0000297295
Regions
	Region	55 – 56	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	192	1	Proton acceptor By similarity
	Metal binding	55	1	Magnesium By similarity
	Binding site	123	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SDV6-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 021B55B98ABD8F6D	FASTA	294	31,928
	10 20 30 40 50 60 MSDAAAAPPE RKKRTITEIR DSKKSGEKMV YTSVPDYTSA KWAELAGVDV AVVGDSLAMV 70 80 90 100 110 120 AHGHSSTVPA TMDMMVMHAQ AVRRGAPRTF TLGCMPYQSY NTVDRALLNA TRFMQDGGCD 130 140 150 160 170 180 AVKPQGGRSQ AHILKALVDS GIPTASHIGL TPHTIAMFGG FKIQGRTAEA AMKILEDAFA 190 200 210 220 230 240 IQDAGCFMLE FEAVPAKIAT LISKQLEIPT IGIGAGAGCD GQILLSYDLL GVFTDFKPKF 250 260 270 280 290 TKRYANLTEV AVQGLKAYVD EVKTGRFPDD DHSYGVDDRE YEQFMNLVEK RRHV

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed: 17158667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000555 Genomic DNA. Translation: ABM93745.1.
RefSeq	YP_001019980.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2SDV6.
Genome annotation databases
GeneID	4784171.
GenomeReviews	Gene locus Mpe_A0783 in contig CP000555_GR.
KEGG	mpt:Mpe_A0783.
NMPDR	fig|279263.3.peg.4102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MACRISA.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB1_METPP

Accession

Primary (citable) accession number: A2SDV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	March 6, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)