true2008-04-292024-03-2793RUVC_METPPWhole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1.Kane S.R.Chakicherla A.Y.Chain P.S.G.Schmidt R.Shin M.W.Legler T.C.Scow K.M.Larimer F.W.Lucas S.M.Richardson P.M.Hristova K.R.doi:10.1128/jb.01259-062007J. Bacteriol.1891931-1945NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]ATCC BAA-1232 / LMG 22953 / PM1BacteriaRuvCRibonuclease H-like superfamily/Ribonuclease HRuvCRNaseH-like_sfRNaseH_sfX-over_junc_endoDNase_Mg_BSX-over_junc_endoDNase_RuvCruvCCROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVCCROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVCRuvCRSOLVASERUVCRibonuclease H-likeRUVCCrossover junction endodeoxyribonuclease RuvC3.1.21.10Holliday junction nuclease RuvCHolliday junction resolvase RuvCruvCMpe_A0482The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.Binds 2 Mg(2+) ion per subunit.Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.Belongs to the RuvC family.Crossover junction endodeoxyribonuclease RuvC192731184971143122007-03-0611927349d9e85e71ff1ae10ec558c2be539632MATRILGIDPGLRTTGFGVVECEGSRLHYIASGTVRTDALPNGDLPGRLKIIFEGVREVAARYQPDCAAVEIVFVNVNPQSTLLLGQARGAALAALVSGDLAVAEYTALQMKKAIVGHGHARKEQVQAMVARLLSLPGEPSRDAADALGLAVCHAHAGASQAAIAAVSPLARRAHAQYRKGRSYtrue