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A2SCY7 (DDL_METPP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Mpe_A0464
OrganismMethylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]
Taxonomic identifier420662 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesMethylibium

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341131

Regions

Domain109 – 313205ATP-grasp
Nucleotide binding139 – 19456ATP By similarity

Sites

Metal binding2661Magnesium or manganese 1 By similarity
Metal binding2801Magnesium or manganese 1 By similarity
Metal binding2801Magnesium or manganese 2 By similarity
Metal binding2821Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A2SCY7 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 7FFB5FB71C5EA40E

FASTA31934,333
        10         20         30         40         50         60 
MSATPDPRSF GKVAVLMGGT SAERDISLMS GGGVLKALQD AGVDAHAFDP KDHELVELRR 

        70         80         90        100        110        120 
QGFQRCFIAL HGRHGEDGTV QGALELLRIP YTGSGVMASA IAMDKIMTKR VWLAEGLPTP 

       130        140        150        160        170        180 
RYVRLAPDEQ TPERVRGVPD DLGLPLIVKP PREGSSIGVT KVLGYSQMQD AVALSARHDP 

       190        200        210        220        230        240 
DVLCEEFIDG AEVTCPVLGE GANARALPVI RIVPPEAGYD YQNKYFTDEV KYLCPSGLPA 

       250        260        270        280        290        300 
DEEAEIQRIV LAAYRALGCR GWGRADLMIR ASDRKPFLLE MNTSPGMTGH SLVPMSAKAA 

       310 
GLGYEQLCLH ILQGAALDA 

« Hide

References

[1]"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1."
Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R.
J. Bacteriol. 189:1931-1945(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000555 Genomic DNA. Translation: ABM93426.1.
RefSeqYP_001019661.1. NC_008825.1.

3D structure databases

ProteinModelPortalA2SCY7.
SMRA2SCY7. Positions 11-315.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING420662.Mpe_A0464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM93426; ABM93426; Mpe_A0464.
GeneID4784183.
KEGGmpt:Mpe_A0464.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycMPET420662:GHBE-470-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_METPP
AccessionPrimary (citable) accession number: A2SCY7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 6, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways