A2SCE2 (PROA_METPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Mpe_A0269

Organism

Methylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]

Taxonomic identifier

420662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Methylibium

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000340893

Sequences

	Sequence		Length	Mass (Da)
	A2SCE2 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 9012649954989C42	FASTA	429	44,948
	10 20 30 40 50 60 MNAPDATPVI ALMDRLGSAA RSASTAMAAA STAAKNAALL ALAREIRAGA ARLAAENARD 70 80 90 100 110 120 LDVATRAGLA APMVDRLRLT DKVIALMAEG CEQVAALPDP IGEMTNLRRR PSGITVGQMR 130 140 150 160 170 180 VPLGVFGMVY ESRPNVTIDA ASLAIKSGNA AILRGGSEAL HSNTALMALV VRALAEAGLP 190 200 210 220 230 240 GDAVQLVPTT DRAAVGRLIA MPQYVDVIIP RGGKGLIERI AAEATVPVIK HLDGNCHVYV 250 260 270 280 290 300 DAGADLDLAV RVTDNAKTQK YSPCNAAESL LVHRDVAAAF LPRIGAVFAA KGVEMRCGPR 310 320 330 340 350 360 AKALLAAVPG AKLVDATEAD WAEEYLAPVI SIKLVDSLDE AIARINRYGS HHTDAILTTN 370 380 390 400 410 420 HPNAMRFLRE VDSASVMVNA STRFADGFEF GLGAEIGIST DKFHARGPVG LEGLTSMKWV VFGQGEVRT

References

[1]

"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1." Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R. J. Bacteriol. 189:1931-1945(2007) [PubMed: 17158667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000555 Genomic DNA. Translation: ABM93231.1.
RefSeq	YP_001019466.1. NC_008825.1.
3D structure databases
ProteinModelPortal	A2SCE2.
ModBase	Search...
Protein-protein interaction databases
STRING	A2SCE2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4786938.
GenomeReviews	Gene locus Mpe_A0269 in contig CP000555_GR.
KEGG	mpt:Mpe_A0269.
NMPDR	fig|279263.3.peg.185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	YGICGAM.
PhylomeDB	A2SCE2.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	MPET420662:MPE_A0269-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_METPP

Accession

Primary (citable) accession number: A2SCE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 6, 2007
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program