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A2SC91 (SYL_METPP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mpe_A0218
OrganismMethylibium petroleiphilum (strain PM1) [Complete proteome] [HAMAP]
Taxonomic identifier420662 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesMethylibium

Protein attributes

Sequence length897 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 897897Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334771

Regions

Motif49 – 5911"HIGH" region HAMAP-Rule MF_00049
Motif654 – 6585"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6571ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A2SC91 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 1C9F65043FCFB22D

FASTA89798,705
        10         20         30         40         50         60 
MSTPHPAPAS AYEPGAIEAA AHAHWTARDA HRVVEDTSRP KFYACSMLPY PSGKLHMGHV 

        70         80         90        100        110        120 
RNYTINDMMA RYLRMKGYNV LMPMGWDAFG LPAENAAIDK KVAPAQWTRD NIADMKSQMQ 

       130        140        150        160        170        180 
PLGLAFDWSR EVATCDPDYY KWNQWFFLKL LEKGIAYKKT QVVNWDPVDQ TVLANEQVID 

       190        200        210        220        230        240 
GRGWRSGAPV ERREIPGYYL AITQYADELL ANVADPASPG FLHGWPERVR LMQEHWIGKS 

       250        260        270        280        290        300 
AGVRFAFPHE IRDADGTLVG DGLMHVFTTR ADTIMGVTFC AVAPEHPLAT HAAASDPDLA 

       310        320        330        340        350        360 
AFIETCKAGG TTEAELATKD KEGRPTGLFV KHPLSGAPVP VWVGNYVLMG YGDGAVMGVP 

       370        380        390        400        410        420 
AHDERDFAFA KKYGIDILQV VHVDGEHFSY DHWQDWYADK QRGVTINSGN LSGLSYPVAV 

       430        440        450        460        470        480 
DAVAAALAAQ GLGDKKTTWR LRDWGISRQR YWGTPIPIIH CTGSGPGSDN PGCGDVPVPE 

       490        500        510        520        530        540 
QDLPVLLPED LIPDGSGNPL NKSAAFLNVA CPKCGAPAKR ETDTMDTFVD SSWYYMRYCC 

       550        560        570        580        590        600 
PDSDAAMVDS RNDYWMPMDQ YIGGIEHAVL HLLYARFWTK AMRDCGLVKF DEPFTKLFTQ 

       610        620        630        640        650        660 
GMLLNESYYR EDASGKKRWF YPSEVEVQFD DKGHPVGAIA REDGQPVQLG GIEKMSKSKN 

       670        680        690        700        710        720 
NVVEPRDIIA KFGADTARLF TMFAGPPDQS AAWSDSGAEG SFRYLRRLWA FATKQREAVQ 

       730        740        750        760        770        780 
AAGTDFADAS RAAIDLRRDV HLLLRQVSHD YDRLQYNTVV SGAMKLLNAL ESAALADTAA 

       790        800        810        820        830        840 
DRAALREGLG ILLRALYPAA PHIAHALWQD LGYAAAHGDL LDAPWPAVDE QALVQDEIEL 

       850        860        870        880        890 
VLQVNGKLRG ALKVPASADR AAIEAAALAS PELAKFAEGR TPKKVVVVPG RLVNVVV 

« Hide

References

[1]"Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1."
Kane S.R., Chakicherla A.Y., Chain P.S.G., Schmidt R., Shin M.W., Legler T.C., Scow K.M., Larimer F.W., Lucas S.M., Richardson P.M., Hristova K.R.
J. Bacteriol. 189:1931-1945(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000555 Genomic DNA. Translation: ABM93180.1.
RefSeqYP_001019415.1. NC_008825.1.

3D structure databases

ProteinModelPortalA2SC91.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING420662.Mpe_A0218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM93180; ABM93180; Mpe_A0218.
GeneID4784003.
KEGGmpt:Mpe_A0218.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycMPET420662:GHBE-218-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METPP
AccessionPrimary (citable) accession number: A2SC91
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 6, 2007
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries