Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

BMA10229_A3218

Organism
Burkholderia mallei (strain NCTC 10229)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102SubstrateBy similarity1
Metal bindingi103Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:BMA10229_A3218
OrganismiBurkholderia mallei (strain NCTC 10229)
Taxonomic identifieri412022 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000002283 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000138261 – 165Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolaseAdd BLAST165

Interactioni

Subunit structurei

Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA2SB35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 83Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

HOGENOMiHOG000252803.
KOiK02553.
OMAiEDNTLVR.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

A2SB35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMFATTDLCD AHEDRLAAGT LRVLEPVFRP FGGVRRFAGP AATLKLFEDN
60 70 80 90 100
SLVRTALEQD GAGRVLVVDG GGSLRCALVG GNLGKLAEKN GWAGIVVNGC
110 120 130 140 150
VRDSDELAEC RVGVLALAAH PRKSDKRGAG VSDAPVDVRG TRIVPGDWIY
160
ADADGVLVSD DALLE
Length:165
Mass (Da):17,301
Last modified:March 6, 2007 - v1
Checksum:iACCDBB82E0923ACA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000546 Genomic DNA. Translation: ABN03182.1.
RefSeqiWP_004191744.1. NC_008836.1.

Genome annotation databases

EnsemblBacteriaiABN03182; ABN03182; BMA10229_A3218.
KEGGibml:BMA10229_A3218.
PATRICi19137450. VBIBurMal46188_5410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000546 Genomic DNA. Translation: ABN03182.1.
RefSeqiWP_004191744.1. NC_008836.1.

3D structure databases

ProteinModelPortaliA2SB35.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN03182; ABN03182; BMA10229_A3218.
KEGGibml:BMA10229_A3218.
PATRICi19137450. VBIBurMal46188_5410.

Phylogenomic databases

HOGENOMiHOG000252803.
KOiK02553.
OMAiEDNTLVR.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAAH_BURM9
AccessioniPrimary (citable) accession number: A2SB35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.