A2SAF0 (PANC_BURM9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	BMA10229_A2975

Organism

Burkholderia mallei (strain NCTC 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

412022 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia mallei

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP-Rule MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP-Rule MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP-Rule MF_00158
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP-Rule MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Pantothenate synthetase HAMAP-Rule MF_00158		PRO_1000097038
Regions
	Nucleotide binding	26 – 33	8	ATP By similarity
	Nucleotide binding	144 – 147	4	ATP By similarity
	Nucleotide binding	181 – 184	4	ATP By similarity
Sites
	Active site	33	1	Proton donor By similarity
	Binding site	57	1	Beta-alanine By similarity
	Binding site	57	1	Pantoate By similarity
	Binding site	150	1	Pantoate By similarity
	Binding site	173	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2SAF0 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: E5C90D9C6DB1001F	FASTA	279	31,207
	10 20 30 40 50 60 MKVISSIQEL RDQLRGQNRT AFVPTMGNLH DGHLSLMRLA RQHGDPVVAS IFVNRLQFGP 70 80 90 100 110 120 NEDFDQYPRT LQDDIEKLQK ENVYVLFAPT ERDMYPEPQE YRVQPPHDLG DILEGEFRPG 130 140 150 160 170 180 FFTGVCTVVT KLMACVQPRV AVFGKKDYQQ LMIVRRMCQQ LALPVEIIAA ETVRDADGLA 190 200 210 220 230 240 LSSRNRYLSE AERAEAPELA KTLAQVRSAV LGGERDLAAI EQRALAHLAA RGWKPDYVSI 250 260 270 RRRANLVAPS AAHIEAGEPL VVLTAAKLGA TRLIDNLEI

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 10229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000546 Genomic DNA. Translation: ABN01369.1.
RefSeq	YP_001028919.1. NC_008836.1.
3D structure databases
ProteinModelPortal	A2SAF0.
ModBase	Search...
Protein-protein interaction databases
STRING	412022.BMA10229_A2975.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN01369; ABN01369; BMA10229_A2975.
GeneID	4791546.
KEGG	bml:BMA10229_A2975.
PATRIC	19136966. VBIBurMal46188_5174.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HOG000175516.
KO	K01918.
OMA	HTIVSVF.
ProtClustDB	PRK00380.
Enzyme and pathway databases
BioCyc	BMAL412022:GJI8-2975-MONOMER.
UniPathway	UPA00028; UER00005.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00158. PanC.
InterPro	IPR004821. Cyt_trans-like. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_BURM9

Accession

Primary (citable) accession number: A2SAF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program