A2S9Y2 (FABH_BURM9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	BMA10229_A2802

Organism

Burkholderia mallei (strain NCTC 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

412022 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia mallei

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP-Rule MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP-Rule MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP-Rule MF_01815
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP-Rule MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP-Rule MF_01815		PRO_1000070218
Regions
	Region	257 – 261	5	ACP-binding By similarity
Sites
	Active site	123	1	By similarity
	Active site	256	1	By similarity
	Active site	286	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2S9Y2 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 3098E02DA5714697	FASTA	329	34,823
	10 20 30 40 50 60 MAQSTLYSRV LGTGSYLPPD RVTNQELADR LAKDGIETSD EWIVARTGIR ARHFAAPDVT 70 80 90 100 110 120 TSDLALVAAQ RAIEAADVDP QSIDLIIVAT STPDFVFPST ACLLQNKLGI KNGGAAFDVQ 130 140 150 160 170 180 AVCSGFAYAL ATADSFIRTG QHRTALVIGA EAFSRILDFK DRTTCVLFGD GAGAVVLSAS 190 200 210 220 230 240 EEPGILGSAL HADGSYSNIL CTPGNVNRGV IAGSAFLHMD GQAVFKLAVN VLEKVAVEAL 250 260 270 280 290 300 SKAELASEQV DWLIPHQANI RIMTSTCRKL GLPQERMIVT VDEHGNTSAA SIPLALDVAV 310 320 RDGRIKRGQH VLIEGVGGGF TWGASVFRF

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 10229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000546 Genomic DNA. Translation: ABN02604.1.
RefSeq	YP_001028751.1. NC_008836.1.
3D structure databases
ProteinModelPortal	A2S9Y2.
SMR	A2S9Y2. Positions 6-329.
ModBase	Search...
Protein-protein interaction databases
STRING	412022.BMA10229_A2802.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN02604; ABN02604; BMA10229_A2802.
GeneID	4791886.
KEGG	bml:BMA10229_A2802.
PATRIC	19136602. VBIBurMal46188_4996.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0332.
HOGENOM	HOG000246674.
KO	K00648.
OMA	AHIVEET.
ProtClustDB	PRK09352.
Enzyme and pathway databases
BioCyc	BMAL412022:GJI8-2802-MONOMER.
UniPathway	UPA00094.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
HAMAP	MF_01815. FabH.
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_BURM9

Accession

Primary (citable) accession number: A2S9Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program