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A2S8K4 (QUEF_BURM9) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13
Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase
Gene names
Name:queF
Ordered Locus Names:BMA10229_A2311
OrganismBurkholderia mallei (strain NCTC 10229) [Complete proteome] [HAMAP]
Taxonomic identifier412022 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817

Subcellular location

Cytoplasm Probable HAMAP MF_00817.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionoxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Inferred from electronic annotation. Source: InterPro

preQ1 synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817
PRO_1000062331

Sequences

Sequence LengthMass (Da)Tools
A2S8K4 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 53ECAF5D759A9272

FASTA27430,401
        10         20         30         40         50         60 
MNPEHSPLGK ATVYANQYDA SLLFPIPRAG AREQIGIGAP LPFFGTDIWN AYELSWLNAR 

        70         80         90        100        110        120 
GKPQIAIATF YVPAESPNIV ESKSFKLYLG SFAQTAFESA DAVRDALKRD VSAACGASVT 

       130        140        150        160        170        180 
VRLATPAEFR KLQMDELDGL SLDRLDLDAH VYETDPSFLT ASHDEAPVEE TLVTDLLKSN 

       190        200        210        220        230        240 
CPVTGQPDWG SVQIHYVGAP IDHAGLLRYI ISFRNHTGFH EQCVERIFVD ILRACQPVKL 

       250        260        270 
AVYARYTRRG GLDINPFRTN YNQPMPDNAR TARQ

« Hide

References

[1]DeShazer D., Woods D.E., Nierman W.C.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 10229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000546 Genomic DNA. Translation: ABN01995.1.
RefSeqYP_001028273.1. NC_008836.1.

3D structure databases

ProteinModelPortalA2S8K4.
SMRA2S8K4. Positions 14-274.
ModBaseSearch...

Protein-protein interaction databases

STRINGA2S8K4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4793456.
GenomeReviewsGene locus BMA10229_A2311 in contig CP000546_GR.
KEGGbml:BMA10229_A2311.
PATRIC19135600. VBIBurMal46188_4504.
TIGRBMA10229_A2311.

Phylogenomic databases

HOGENOMHBG289139.
OMACVERIFC.
PhylomeDBA2S8K4.
ProtClustDBPRK11792.

Family and domain databases

HAMAPMF_00817. QueF_type2.
[Tree]
InterProIPR016428. CN_OxRdtase_NADPH-dep_YqcD.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
[Graphical view]
KOK06879.
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsTIGR03138. QueF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_BURM9
AccessionPrimary (citable) accession number: A2S8K4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 6, 2007
Last modified: December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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