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A2S862

- DAPF_BURM9

UniProt

A2S862 - DAPF_BURM9

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Burkholderia mallei (strain NCTC 10229)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei47 – 471SubstrateUniRule annotation
    Binding sitei67 – 671SubstrateUniRule annotation
    Active sitei76 – 761Proton donor/acceptorUniRule annotation
    Binding sitei167 – 1671SubstrateUniRule annotation
    Sitei169 – 1691Important for catalytic activityUniRule annotation
    Binding sitei200 – 2001SubstrateUniRule annotation
    Sitei218 – 2181Important for catalytic activityUniRule annotation
    Active sitei227 – 2271Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciBMAL412022:GJI8-2167-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:BMA10229_A2167
    OrganismiBurkholderia mallei (strain NCTC 10229)
    Taxonomic identifieri412022 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000002283: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 289289Diaminopimelate epimerasePRO_1000011855Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi76 ↔ 227

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi412022.BMA10229_A2167.

    Structurei

    3D structure databases

    ProteinModelPortaliA2S862.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 783Substrate bindingUniRule annotation
    Regioni218 – 2192Substrate bindingUniRule annotation
    Regioni228 – 2292Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiKDITVMV.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A2S862-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSFTKMHG AGNDFVVLDG YTRALPPLTG AQVRALADRH FGIGADQLLL    50
    VEKPTVDGAD FKYRIFNCDG GEVEHCGNGA RCFVKFVRDH GLTGKASVRV 100
    EVKHGVITLT MQDNGEVVVD MGAPVFEPAR VPFDASGLDG RREGADTLWP 150
    LPVNGVTRWI SVVSMGNPHA VQIVDDAEAF AVRVDGPAIE RDPRFPQRVN 200
    AGFMQIVSRH EVNLRVYERG AGETLACGTG ACAAVAAGIR RGRLDSPVTV 250
    HTHGGTLTIS WNGACDERAP LMMAGPATTV FEGVIELPA 289
    Length:289
    Mass (Da):30,899
    Last modified:March 6, 2007 - v1
    Checksum:i7A21ABF99D793D82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000546 Genomic DNA. Translation: ABN02872.1.
    RefSeqiYP_001028131.1. NC_008836.1.

    Genome annotation databases

    EnsemblBacteriaiABN02872; ABN02872; BMA10229_A2167.
    GeneIDi4791787.
    KEGGibml:BMA10229_A2167.
    PATRICi19135312. VBIBurMal46188_4362.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000546 Genomic DNA. Translation: ABN02872.1 .
    RefSeqi YP_001028131.1. NC_008836.1.

    3D structure databases

    ProteinModelPortali A2S862.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 412022.BMA10229_A2167.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN02872 ; ABN02872 ; BMA10229_A2167 .
    GeneIDi 4791787.
    KEGGi bml:BMA10229_A2167.
    PATRICi 19135312. VBIBurMal46188_4362.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi KDITVMV.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci BMAL412022:GJI8-2167-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. DeShazer D., Woods D.E., Nierman W.C.
      Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 10229.

    Entry informationi

    Entry nameiDAPF_BURM9
    AccessioniPrimary (citable) accession number: A2S862
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3