Reviewed, UniProtKB/Swiss-Prot A2S7R2 (BIOD_BURM9)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	BMA10229_A2016

Organism

Burkholderia mallei (strain NCTC 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

412022 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Dethiobiotin synthetase HAMAP MF_00336		PRO_1000019551
Regions
	Nucleotide binding	11 – 19	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2S7R2-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 5E28F2256905A6FB	FASTA	240	24,874
	10 20 30 40 50 60 MSAPLSLFVT GTDTEIGKTF VSAALLHGFA RAGLRAAAMK PVAAGAYERD GAWRNEDADQ 70 80 90 100 110 120 LDAAANVALP AAIRTPFLLK APAAPHIVAA REGVALDIGT IVDAHRRACE MADVIVVEGV 130 140 150 160 170 180 GGVRVPLADT RDTADLAVAL GLPVVLVVGV RLGCISHALL TAEAIAARGL PLAGWVANRI 190 200 210 220 230 240 DPAMPFADDN IDTLRAWLER EHRAPLLGAL AHMSPPSPDA ASHALDVNLL LNALRAAAPR

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000546 Genomic DNA. Translation: ABN03855.1.
RefSeq	YP_001027981.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4792374.
GenomeReviews	Gene locus BMA10229_A2016 in contig CP000546_GR.
KEGG	bml:BMA10229_A2016.
TIGR	BMA10229_A2016.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	ETPEGMR.
PhylomeDB	A2S7R2.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BURM9

Accession

Primary (citable) accession number: A2S7R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)