A2S5U7 (MURD_BURM9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	BMA10229_A1333

Organism

Burkholderia mallei (strain NCTC 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

412022 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000056874
Regions
	Nucleotide binding	129 – 135	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A2S5U7 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 5F5BD80F4EB8C5D7	FASTA	504	52,549
	10 20 30 40 50 60 MFGDRQRPMV LVLGLGESGL AIARWCARHG CRLRVADTRE TPPNLAALTA AGVDFEFVGG 70 80 90 100 110 120 AFSPALVDGG IELVALSPGL SPLAEDLAPL VAAARERGIP VWGELEFFAQ ALAALGANGY 130 140 150 160 170 180 APKVIAITGT NGKTTTTSLA GLLCERAGKK VAVAGNISPA MLDKLTEAID AAALPDVWVL 190 200 210 220 230 240 ELSSFQLDTA HTFAPDAATI LNITQDHLDW HGGFAAYAAA KGRVFGPRTV RVLNRDDAEV 250 260 270 280 290 300 MRFAPPAAAA DAPRAVTFGL NEPAADGDYG LLRENGIAWL VEAIDRDGAD APAAPSRRRK 310 320 330 340 350 360 QEAANPPDIA LKRLMPADAL RIRGLHNAAN ALAAYALARA IGLPAAPLLH GLREYRGEPH 370 380 390 400 410 420 RVEVIATLDG VDYVDDSKGT NVGATVAALD GLAQRAVLIA GGDGKGQDFE PLAAPVARWC 430 440 450 460 470 480 RAVMLIGRDA PALREALADT GVPLADHATL EAAVRAASAL AQPGDAVLLS PACASLDMFR 490 500 NYAHRADVFR SAVEDIALEK GTTL

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 10229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000546 Genomic DNA. Translation: ABN03662.1.
RefSeq	YP_001027316.1. NC_008836.1.
3D structure databases
ProteinModelPortal	A2S5U7.
ModBase	Search...
Protein-protein interaction databases
STRING	A2S5U7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4793862.
GenomeReviews	Gene locus BMA10229_A1333 in contig CP000546_GR.
KEGG	bml:BMA10229_A1333.
PATRIC	19133626. VBIBurMal46188_3532.
TIGR	BMA10229_A1333.
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
PhylomeDB	A2S5U7.
ProtClustDB	PRK02006.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BURM9

Accession

Primary (citable) accession number: A2S5U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program