Reviewed, UniProtKB/Swiss-Prot A2S4B5 (HEM6_BURM9)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	BMA10229_A0794

Organism

Burkholderia mallei (strain NCTC 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

412022 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000019459
Regions
	Region	55 – 64	10	Important for dimerization By similarity
	Region	115 – 117	3	Substrate binding By similarity
	Region	247 – 282	36	Important for dimerization By similarity
	Region	265 – 270	6	Substrate binding By similarity
Sites
	Active site	113	1	Proton donor By similarity
	Binding site	99	1	Substrate By similarity
	Site	182	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2S4B5-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 095790F8C2662CD0	FASTA	307	34,596
	10 20 30 40 50 60 MTDSTYDVNR VRAYLQGLQM RIADALGAFD GTPLAADTWR RGPGERLRGG GCTRILEAGG 70 80 90 100 110 120 FFERAGIGFS DVAGDALPPS ANASRPQLAG RGFEALGVSL VLHPRNPYCP TVHMNVRMLI 130 140 150 160 170 180 ATKPGEAPVF WFGGGMDLTP IYGFEEDARH FHRTCRAALE PFGAELYPRF KKWCDDYFFL 190 200 210 220 230 240 KHRNEARGIG GIFFDDFSEL GFERSFEMLQ SVGDAFLPSY LPIVERRRDT PYGERERAFQ 250 260 270 280 290 300 AYRRGRYVEF NLVFDRGTLF GLQSGGRTES ILLSMPPTAG WRYDWHPDPG TPEARLQSEF LVPRDWA

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000546 Genomic DNA. Translation: ABN03659.1.
RefSeq	YP_001026784.1.
3D structure databases
SMR	A2S4B5. Positions 5-307.
ModBase	Search...
Genome annotation databases
GeneID	4792662.
GenomeReviews	Gene locus BMA10229_A0794 in contig CP000546_GR.
KEGG	bml:BMA10229_A0794.
TIGR	BMA10229_A0794.
Phylogenomic databases
HOGENOM	HBG631180.
OMA	HMNTRFV.
PhylomeDB	A2S4B5.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_BURM9

Accession

Primary (citable) accession number: A2S4B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	January 19, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)