A2S233 (PHNW_BURM9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-aminoethylphosphonate--pyruvate transaminase
EC=2.6.1.37

Alternative name(s):
2-aminoethylphosphonate aminotransferase
AEP transaminase
Short name=AEPT

Gene names

Name:	phnW
Ordered Locus Names:	BMA10229_2208

Organism

Burkholderia mallei (strain NCTC 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

412022 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in phosphonate degradation By similarity. HAMAP MF_01376
Catalytic activity	(2-aminoethyl)phosphonate + pyruvate = 2-phosphonoacetaldehyde + L-alanine. HAMAP MF_01376
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01376
Subunit structure	Homodimer By similarity. HAMAP MF_01376
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. PhnW subfamily.

Ontologies

Keywords
Ligand	Pyridoxal phosphate Pyruvate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	organic phosphonate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	2-aminoethylphosphonate-pyruvate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	2-aminoethylphosphonate--pyruvate transaminase HAMAP MF_01376		PRO_1000068244
Amino acid modifications
	Modified residue	193	1	N6-(pyridoxal phosphate)lysine Potential

Sequences

	Sequence		Length	Mass (Da)
	A2S233 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 8136927349BAF6F6	FASTA	369	38,859
	10 20 30 40 50 60 MPERDPILLT PGPLTTSRMT RDAMLRDWGS WDAAFNRLTK SVCADLVRIA GGGDAYVCVP 70 80 90 100 110 120 LQGSGTFAVE ATLGTLVPRD ARVLVPNNGA YCARIAAILR RLGIAHVELP FAEDEPASAH 130 140 150 160 170 180 AIDAALARDA RLTHVALVHL ETSAGLLNPL DDIAAVCRAR GRALIVDAMS SFGALPIALA 190 200 210 220 230 240 ASGIDALISA SGKCLEGVPG MGFAIVRRSA LEAAEGRSPS VALDLHDQYA YMQRTSQWRF 250 260 270 280 290 300 TPPTHVLAAL RAALDQFFDE GGQPARGARY ARNCATLVDG MRALGFEPFL DARAQASVIV 310 320 330 340 350 360 TFYAPADPAY AFPAFYAAVR DAGYVLYPGK LTTADTFRVG CIGALGADEM RGAVAAIGGA LESLGIAMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 10229.
[2]	Brinkac L.M., Harkins D.M., Shrivastava S., Durkin A.S., Sutton G. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000545 Genomic DNA. Translation: ABM99748.2.
RefSeq	YP_001026000.1. NC_008835.1. YP_001026002.1. NC_008835.1.
3D structure databases
ProteinModelPortal	A2S233.
ModBase	Search...
Protein-protein interaction databases
STRING	A2S233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4790273. 4790304.
GenomeReviews	Gene locus BMA10229_2208 in contig CP000545_GR.
KEGG	bml:BMA10229_2208.
PATRIC	19130861. VBIBurMal46188_2160.
TIGR	BMA10229_2208.
Phylogenomic databases
HOGENOM	HBG423997.
OMA	ITHVAQV.
Family and domain databases
HAMAP	MF_01376. PhnW_aminotrans_5. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR012703. NH2EtPonate_pyrv_transaminase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR024169. SP_NH2Trfase/AEP_transaminase. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00014.
PANTHER	PTHR21152:SF6. PTHR21152:SF6. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000524. SPT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR03301. PhnW-AepZ. 1 hit. TIGR02326. Transamin_PhnW. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHNW_BURM9

Accession

Primary (citable) accession number: A2S233
Secondary accession number(s): A2S231

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 6, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program