A2RXU4 (NADE_BURM9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BMA10229_0698

Organism

Burkholderia mallei (strain NCTC 10229) [Complete proteome] [HAMAP]

Taxonomic identifier

412022 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099007
Regions
	Nucleotide binding	51 – 58	8	ATP By similarity
Sites
	Active site	53	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2RXU4 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 8AE2C18E0B0BCEAF	FASTA	284	30,863
	10 20 30 40 50 60 MSRPDQAARR RAIAAELHVS PTFDARDEAE RRIGFVADYL RTAGLRACVL GISGGIDSST 70 80 90 100 110 120 AGRLAQLAVE RLRASGYDAR FVAMRLPYGA QHDEADARRA LAFVRADETL TVDVKPAADA 130 140 150 160 170 180 MLAALAAGGL AYLDHAQQDF VLGNIKARER MIAQYAVAGA RNGVVIGTDH AAESVMGFFT 190 200 210 220 230 240 KFGDGGADVL PLAGLTKRRV RALARMLGAD EPLVLKTPTA DLETLRPQRP DEHAYGITYE 250 260 270 280 QIDDFLEGKP MDDAVAETVL RFYDATHHKR ALPYTMFDWP GHPA

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 10229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000545 Genomic DNA. Translation: ABM99595.1.
RefSeq	YP_001024513.1. NC_008835.1.
3D structure databases
ProteinModelPortal	A2RXU4.
SMR	A2RXU4. Positions 11-278.
ModBase	Search...
Protein-protein interaction databases
STRING	A2RXU4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4789317.
GenomeReviews	Gene locus BMA10229_0698 in contig CP000545_GR.
KEGG	bml:BMA10229_0698.
PATRIC	19127905. VBIBurMal46188_0685.
TIGR	BMA10229_0698.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	IAQYEIA.
PhylomeDB	A2RXU4.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BURM9

Accession

Primary (citable) accession number: A2RXU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 6, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program