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A2RVP7 (TI221_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM22-1
Alternative name(s):
Protein MATERNAL EFFECT EMBRYO ARREST 67
Gene names
Name:TIM22-1
Synonyms:MEE67
Ordered Locus Names:At3g10110
ORF Names:T22K18.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length173 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Ref.1

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Potential Ref.1.

Tissue specificity

Expressed in young cotyledons, roots, flowers and leaves. Ref.5 Ref.6

Induction

Up-regulated after antimycin A or rotenone treatments. Ref.6

Disruption phenotype

Embryo lethality. Ref.7

Sequence similarities

Belongs to the Tim17/Tim22/Tim23 family.

Sequence caution

The sequence AAF04413.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1818Mitochondrion Potential
Chain19 – 173155Mitochondrial import inner membrane translocase subunit TIM22-1
PRO_0000420934

Regions

Transmembrane52 – 7221Helical; Potential
Transmembrane101 – 11919Helical; Potential
Transmembrane128 – 14417Helical; Potential
Transmembrane151 – 16818Helical; Potential

Experimental info

Sequence conflict91P → T in ABD64057. Ref.1
Sequence conflict91P → T in ABD64058. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A2RVP7 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: CFC24979387481AB

FASTA17318,183
        10         20         30         40         50         60 
MADSSAAEPT TGASSPPVAS DENSTQIQPI RMPTIEEIRA QEVWNNCAVR AVTSGVMGGG 

        70         80         90        100        110        120 
LGLMMGLFLG ALDNPITHDT MTARQQFVFT AKQMGQRSWN SCKTFAVMGL VFSAAECIVE 

       130        140        150        160        170 
KARAKHDTVN TAIAGCVTGG SMSARGGPKA ACIGCAGFAT FSVLIEKFFD RHT 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the preprotein and amino acid transporter gene family in Arabidopsis."
Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M., Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.
Plant Physiol. 143:199-212(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Identification, expression, and import of components 17 and 23 of the inner mitochondrial membrane translocase from Arabidopsis."
Murcha M.W., Lister R., Ho A.Y., Whelan J.
Plant Physiol. 131:1737-1747(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"A transcriptomic and proteomic characterization of the Arabidopsis mitochondrial protein import apparatus and its response to mitochondrial dysfunction."
Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L., Millar A.H., Whelan J.
Plant Physiol. 134:777-789(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[7]"Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis."
Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S., Capron A., Xie L.-F., Ye D., Sundaresan V.
Development 132:603-614(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE.
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ405268 mRNA. Translation: ABD64057.1.
DQ405269 mRNA. Translation: ABD64058.1.
AC010927 Genomic DNA. Translation: AAF04413.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74859.1.
BT030038 mRNA. Translation: ABN04776.1.
RefSeqNP_566368.1. NM_111846.3.
UniGeneAt.39987.
At.41800.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEA2RVP7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G10110.1; AT3G10110.1; AT3G10110.
GeneID820172.
KEGGath:AT3G10110.

Organism-specific databases

GeneFarm3043. 302.
TAIRAT3G10110.

Phylogenomic databases

eggNOGCOG5596.
InParanoidA2RVP7.
KOK17790.
OMAGMESCVA.
PhylomeDBA2RVP7.

Gene expression databases

GenevestigatorA2RVP7.

Family and domain databases

InterProIPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamPF02466. Tim17. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROA2RVP7.

Entry information

Entry nameTI221_ARATH
AccessionPrimary (citable) accession number: A2RVP7
Secondary accession number(s): Q9SR75
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: March 6, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names