Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A2RUS2

- DEND3_HUMAN

UniProt

A2RUS2 - DEND3_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DENN domain-containing protein 3

Gene

DENND3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. Thereby, may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor.1 Publication

GO - Molecular functioni

  1. Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  1. cellular protein catabolic process Source: UniProtKB
  2. endosome to lysosome transport Source: UniProtKB
  3. positive regulation of Rab GTPase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

SignaLinkiA2RUS2.

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 3
Gene namesi
Name:DENND3
Synonyms:KIAA0870
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:29134. DENND3.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671987.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11981198DENN domain-containing protein 3PRO_0000304672Add
BLAST

Proteomic databases

MaxQBiA2RUS2.
PaxDbiA2RUS2.
PRIDEiA2RUS2.

PTM databases

PhosphoSiteiA2RUS2.

Expressioni

Gene expression databases

BgeeiA2RUS2.
CleanExiHS_DENND3.
ExpressionAtlasiA2RUS2. baseline and differential.
GenevestigatoriA2RUS2.

Organism-specific databases

HPAiHPA026100.

Interactioni

Protein-protein interaction databases

BioGridi116562. 3 interactions.
IntActiA2RUS2. 5 interactions.
STRINGi9606.ENSP00000262585.

Structurei

3D structure databases

ProteinModelPortaliA2RUS2.
SMRiA2RUS2. Positions 39-420, 956-1055.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 8378UDENNAdd
BLAST
Domaini105 – 291187DENNPROSITE-ProRule annotationAdd
BLAST
Domaini354 – 41764dDENNPROSITE-ProRule annotationAdd
BLAST
Repeati984 – 102643WD 1Add
BLAST
Repeati1073 – 111341WD 2Add
BLAST
Repeati1165 – 119834WD 3Add
BLAST

Sequence similaritiesi

Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation
Contains 3 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiNOG115977.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000060114.
HOVERGENiHBG095574.
InParanoidiA2RUS2.
PhylomeDBiA2RUS2.
TreeFamiTF331814.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A2RUS2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSLRKKREK PRPEQWKGLP GPPRAPEPED VAVPGGVDLL TLPQLCFPGG
60 70 80 90 100
VCVATEPKED CVHFLVLTDV CGNRTYGVVA QYYRPLHDEY CFYNGKTHRE
110 120 130 140 150
CPGCFVPFAV CVVSRFPYYN SLKDCLSCLL ALLKPCKDFE VDSHIKDFAA
160 170 180 190 200
KLSLIPSPPP GPLHLVFNMK SLQIVLPARA DPESPILDLD LHLPLLCFRP
210 220 230 240 250
EKVLQILTCI LTEQRIVFFS SDWALLTLVT ECFMAYLYPL QWQHPFVPIL
260 270 280 290 300
SDQMLDFVMA PTSFLMGCHL DHFEEVSKEA DGLVLINIDH GSITYSKSTD
310 320 330 340 350
DNVDIPDVPL LAAQTFIQRV QSLQLHHELH AAHLLSSTDL KEGRAHRRSW
360 370 380 390 400
QQKLNCQIQQ TTLQLLVSIF RDVKNHLNYE HRVFNSEEFL KTRAPGDHQF
410 420 430 440 450
YKQVLDTYMF HSFLKARLNR RMDAFAQMDL DTQSEEDRIN GMLLSPRRPT
460 470 480 490 500
VEKRASRKSS HLHVTHRRMV VSMPNLQDIA MPELAPRNSS LRLTDTAGCR
510 520 530 540 550
GSSAVLNVTP KSPYTFKIPE IHFPLESKCV QAYHAHFVSM LSEAMCFLAP
560 570 580 590 600
DNSLLLARYL YLRGLVYLMQ GQLLNALLDF QNLYKTDIRI FPTDLVKRTV
610 620 630 640 650
ESMSAPEWEG AEQAPELMRL ISEILDKPHE ASKLDDHVKK FKLPKKHMQL
660 670 680 690 700
GDFMKRVQES GIVKDASIIH RLFEALTVGQ EKQIDPETFK DFYNCWKETE
710 720 730 740 750
AEAQEVSLPW LVMEHLDKNE CVCKLSSSVK TNLGVGKIAM TQKRLFLLTE
760 770 780 790 800
GRPGYLEIST FRNIEEVRRT TTTFLLRRIP TLKIRVASKK EVFEANLKTE
810 820 830 840 850
CDLWHLMVKE MWAGKKLADD HKDPHYVQQA LTNVLLMDAV VGTLQSPGAI
860 870 880 890 900
YAASKLSYFD KMSNEMPMTL PETTLETLKH KINPSAGEAF PQAVDVLLYT
910 920 930 940 950
PGHLDPAEKV EDAHPKLWCA LSEGKVTVFN ASSWTIHQHS FKVGTAKVNC
960 970 980 990 1000
MVMADQNQVW VGSEDSVIYI INVHSMSCNK QLTAHCSSVT DLIVQDGQEA
1010 1020 1030 1040 1050
PSNVYSCSMD GMVLVWNVST LQVTSRFQLP RGGLTSIRLH GGRLWCCTGN
1060 1070 1080 1090 1100
SIMVMKMNGS LHQELKIEEN FKDTSTSFLA FQLLPEEEQL WAACAGRSEV
1110 1120 1130 1140 1150
YIWSLKDLAQ PPQRVPLEDC SEINCMIRVK KQVWVGSRGL GQGTPKGKIY
1160 1170 1180 1190
VIDAERKTVE KELVAHMDTV RTLCSAEDRY VLSGSGREEG KVAIWKGE
Length:1,198
Mass (Da):135,890
Last modified:September 11, 2007 - v2
Checksum:i52D51635DDD88A4E
GO
Isoform 2 (identifier: A2RUS2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-370: Missing.

Note: No experimental confirmation available.

Show »
Length:1,146
Mass (Da):129,809
Checksum:iB8D7B60A600DDD0C
GO
Isoform 3 (identifier: A2RUS2-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-950: Missing.
     1133-1198: VWVGSRGLGQ...EGKVAIWKGE → VGWRARHPQH...PNWMASNDRT

Note: No experimental confirmation available.

Show »
Length:266
Mass (Da):29,486
Checksum:iB5D62298848A4332
GO
Isoform 4 (identifier: A2RUS2-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     128-128: C → W
     129-1198: Missing.

Note: No experimental confirmation available.

Show »
Length:128
Mass (Da):14,509
Checksum:iB5E0DB642AC3F733
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti143 – 1431S → N.
Corresponds to variant rs307761 [ dbSNP | Ensembl ].
VAR_035053
Natural varianti364 – 3641Q → R.
Corresponds to variant rs11997191 [ dbSNP | Ensembl ].
VAR_035054

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 950950Missing in isoform 3. 1 PublicationVSP_028075Add
BLAST
Alternative sequencei128 – 1281C → W in isoform 4. 1 PublicationVSP_028077
Alternative sequencei129 – 11981070Missing in isoform 4. 1 PublicationVSP_028078Add
BLAST
Alternative sequencei319 – 37052Missing in isoform 2. 1 PublicationVSP_028080Add
BLAST
Alternative sequencei1133 – 119866VWVGS…IWKGE → VGWRARHPQHPRQVSLALAA SPCSREPAARPRALLPSPLR VPLLTGTCRVGANGPTGHHV LVSWCGPSTPQRIPPNWMAS NDRT in isoform 3. 1 PublicationVSP_028081Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK058001 mRNA. Translation: BAB71630.1.
AK131505 mRNA. Translation: BAD18649.1.
AC040970 Genomic DNA. No translation available.
BC133023 mRNA. Translation: AAI33024.1.
BC144226 mRNA. Translation: AAI44227.1.
AB020677 mRNA. Translation: BAA74893.2.
CCDSiCCDS34947.1. [A2RUS2-1]
RefSeqiNP_055772.2. NM_014957.2. [A2RUS2-1]
UniGeneiHs.18166.

Genome annotation databases

EnsembliENST00000262585; ENSP00000262585; ENSG00000105339. [A2RUS2-1]
ENST00000424248; ENSP00000410594; ENSG00000105339. [A2RUS2-2]
GeneIDi22898.
KEGGihsa:22898.
UCSCiuc003yvy.3. human. [A2RUS2-1]
uc003ywa.1. human. [A2RUS2-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK058001 mRNA. Translation: BAB71630.1 .
AK131505 mRNA. Translation: BAD18649.1 .
AC040970 Genomic DNA. No translation available.
BC133023 mRNA. Translation: AAI33024.1 .
BC144226 mRNA. Translation: AAI44227.1 .
AB020677 mRNA. Translation: BAA74893.2 .
CCDSi CCDS34947.1. [A2RUS2-1 ]
RefSeqi NP_055772.2. NM_014957.2. [A2RUS2-1 ]
UniGenei Hs.18166.

3D structure databases

ProteinModelPortali A2RUS2.
SMRi A2RUS2. Positions 39-420, 956-1055.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116562. 3 interactions.
IntActi A2RUS2. 5 interactions.
STRINGi 9606.ENSP00000262585.

PTM databases

PhosphoSitei A2RUS2.

Proteomic databases

MaxQBi A2RUS2.
PaxDbi A2RUS2.
PRIDEi A2RUS2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000262585 ; ENSP00000262585 ; ENSG00000105339 . [A2RUS2-1 ]
ENST00000424248 ; ENSP00000410594 ; ENSG00000105339 . [A2RUS2-2 ]
GeneIDi 22898.
KEGGi hsa:22898.
UCSCi uc003yvy.3. human. [A2RUS2-1 ]
uc003ywa.1. human. [A2RUS2-3 ]

Organism-specific databases

CTDi 22898.
GeneCardsi GC08P142127.
H-InvDB HIX0007822.
HGNCi HGNC:29134. DENND3.
HPAi HPA026100.
neXtProti NX_A2RUS2.
PharmGKBi PA142671987.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG115977.
GeneTreei ENSGT00760000118819.
HOGENOMi HOG000060114.
HOVERGENi HBG095574.
InParanoidi A2RUS2.
PhylomeDBi A2RUS2.
TreeFami TF331814.

Enzyme and pathway databases

SignaLinki A2RUS2.

Miscellaneous databases

GenomeRNAii 22898.
NextBioi 43523.
PROi A2RUS2.

Gene expression databases

Bgeei A2RUS2.
CleanExi HS_DENND3.
ExpressionAtlasi A2RUS2. baseline and differential.
Genevestigatori A2RUS2.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view ]
SMARTi SM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
    Tissue: Gastric mucosa and Uterus.
  2. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  4. "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 180-1146 (ISOFORM 1).
    Tissue: Brain.
  5. "Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors."
    Yoshimura S., Gerondopoulos A., Linford A., Rigden D.J., Barr F.A.
    J. Cell Biol. 191:367-381(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS GUANYL-NUCLEOTIDE EXCHANGE FACTOR.

Entry informationi

Entry nameiDEND3_HUMAN
AccessioniPrimary (citable) accession number: A2RUS2
Secondary accession number(s): B7ZM28
, O94947, Q2TAM7, Q6ZMS6, Q96DK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: October 29, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3