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A2RUS2 (DEND3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DENN domain-containing protein 3
Gene names
Name:DENND3
Synonyms:KIAA0870
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1198 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. Thereby, may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Ref.5

Sequence similarities

Contains 1 dDENN domain.

Contains 1 DENN domain.

Contains 1 uDENN domain.

Contains 3 WD repeats.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A2RUS2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A2RUS2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     319-370: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: A2RUS2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-950: Missing.
     1133-1198: VWVGSRGLGQ...EGKVAIWKGE → VGWRARHPQH...PNWMASNDRT
Note: No experimental confirmation available.
Isoform 4 (identifier: A2RUS2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     128-128: C → W
     129-1198: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11981198DENN domain-containing protein 3
PRO_0000304672

Regions

Domain6 – 8378UDENN
Domain105 – 291187DENN
Domain354 – 41764dDENN
Repeat984 – 102643WD 1
Repeat1073 – 111341WD 2
Repeat1165 – 119834WD 3

Natural variations

Alternative sequence1 – 950950Missing in isoform 3.
VSP_028075
Alternative sequence1281C → W in isoform 4.
VSP_028077
Alternative sequence129 – 11981070Missing in isoform 4.
VSP_028078
Alternative sequence319 – 37052Missing in isoform 2.
VSP_028080
Alternative sequence1133 – 119866VWVGS…IWKGE → VGWRARHPQHPRQVSLALAA SPCSREPAARPRALLPSPLR VPLLTGTCRVGANGPTGHHV LVSWCGPSTPQRIPPNWMAS NDRT in isoform 3.
VSP_028081
Natural variant1431S → N.
Corresponds to variant rs307761 [ dbSNP | Ensembl ].
VAR_035053
Natural variant3641Q → R.
Corresponds to variant rs11997191 [ dbSNP | Ensembl ].
VAR_035054

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: 52D51635DDD88A4E

FASTA1,198135,890
        10         20         30         40         50         60 
MRSLRKKREK PRPEQWKGLP GPPRAPEPED VAVPGGVDLL TLPQLCFPGG VCVATEPKED 

        70         80         90        100        110        120 
CVHFLVLTDV CGNRTYGVVA QYYRPLHDEY CFYNGKTHRE CPGCFVPFAV CVVSRFPYYN 

       130        140        150        160        170        180 
SLKDCLSCLL ALLKPCKDFE VDSHIKDFAA KLSLIPSPPP GPLHLVFNMK SLQIVLPARA 

       190        200        210        220        230        240 
DPESPILDLD LHLPLLCFRP EKVLQILTCI LTEQRIVFFS SDWALLTLVT ECFMAYLYPL 

       250        260        270        280        290        300 
QWQHPFVPIL SDQMLDFVMA PTSFLMGCHL DHFEEVSKEA DGLVLINIDH GSITYSKSTD 

       310        320        330        340        350        360 
DNVDIPDVPL LAAQTFIQRV QSLQLHHELH AAHLLSSTDL KEGRAHRRSW QQKLNCQIQQ 

       370        380        390        400        410        420 
TTLQLLVSIF RDVKNHLNYE HRVFNSEEFL KTRAPGDHQF YKQVLDTYMF HSFLKARLNR 

       430        440        450        460        470        480 
RMDAFAQMDL DTQSEEDRIN GMLLSPRRPT VEKRASRKSS HLHVTHRRMV VSMPNLQDIA 

       490        500        510        520        530        540 
MPELAPRNSS LRLTDTAGCR GSSAVLNVTP KSPYTFKIPE IHFPLESKCV QAYHAHFVSM 

       550        560        570        580        590        600 
LSEAMCFLAP DNSLLLARYL YLRGLVYLMQ GQLLNALLDF QNLYKTDIRI FPTDLVKRTV 

       610        620        630        640        650        660 
ESMSAPEWEG AEQAPELMRL ISEILDKPHE ASKLDDHVKK FKLPKKHMQL GDFMKRVQES 

       670        680        690        700        710        720 
GIVKDASIIH RLFEALTVGQ EKQIDPETFK DFYNCWKETE AEAQEVSLPW LVMEHLDKNE 

       730        740        750        760        770        780 
CVCKLSSSVK TNLGVGKIAM TQKRLFLLTE GRPGYLEIST FRNIEEVRRT TTTFLLRRIP 

       790        800        810        820        830        840 
TLKIRVASKK EVFEANLKTE CDLWHLMVKE MWAGKKLADD HKDPHYVQQA LTNVLLMDAV 

       850        860        870        880        890        900 
VGTLQSPGAI YAASKLSYFD KMSNEMPMTL PETTLETLKH KINPSAGEAF PQAVDVLLYT 

       910        920        930        940        950        960 
PGHLDPAEKV EDAHPKLWCA LSEGKVTVFN ASSWTIHQHS FKVGTAKVNC MVMADQNQVW 

       970        980        990       1000       1010       1020 
VGSEDSVIYI INVHSMSCNK QLTAHCSSVT DLIVQDGQEA PSNVYSCSMD GMVLVWNVST 

      1030       1040       1050       1060       1070       1080 
LQVTSRFQLP RGGLTSIRLH GGRLWCCTGN SIMVMKMNGS LHQELKIEEN FKDTSTSFLA 

      1090       1100       1110       1120       1130       1140 
FQLLPEEEQL WAACAGRSEV YIWSLKDLAQ PPQRVPLEDC SEINCMIRVK KQVWVGSRGL 

      1150       1160       1170       1180       1190 
GQGTPKGKIY VIDAERKTVE KELVAHMDTV RTLCSAEDRY VLSGSGREEG KVAIWKGE 

« Hide

Isoform 2 [UniParc].

Checksum: B8D7B60A600DDD0C
Show »

FASTA1,146129,809
Isoform 3 [UniParc].

Checksum: B5D62298848A4332
Show »

FASTA26629,486
Isoform 4 [UniParc].

Checksum: B5E0DB642AC3F733
Show »

FASTA12814,509

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
Tissue: Gastric mucosa and Uterus.
[2]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[4]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 180-1146 (ISOFORM 1).
Tissue: Brain.
[5]"Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors."
Yoshimura S., Gerondopoulos A., Linford A., Rigden D.J., Barr F.A.
J. Cell Biol. 191:367-381(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS GUANYL-NUCLEOTIDE EXCHANGE FACTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK058001 mRNA. Translation: BAB71630.1.
AK131505 mRNA. Translation: BAD18649.1.
AC040970 Genomic DNA. No translation available.
BC133023 mRNA. Translation: AAI33024.1.
BC144226 mRNA. Translation: AAI44227.1.
AB020677 mRNA. Translation: BAA74893.2.
CCDSCCDS34947.1. [A2RUS2-1]
RefSeqNP_055772.2. NM_014957.2. [A2RUS2-1]
UniGeneHs.18166.

3D structure databases

ProteinModelPortalA2RUS2.
SMRA2RUS2. Positions 39-420, 919-1017.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116562. 3 interactions.
IntActA2RUS2. 5 interactions.
STRING9606.ENSP00000262585.

PTM databases

PhosphoSiteA2RUS2.

Proteomic databases

MaxQBA2RUS2.
PaxDbA2RUS2.
PRIDEA2RUS2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262585; ENSP00000262585; ENSG00000105339. [A2RUS2-1]
ENST00000424248; ENSP00000410594; ENSG00000105339. [A2RUS2-2]
GeneID22898.
KEGGhsa:22898.
UCSCuc003yvy.3. human. [A2RUS2-1]
uc003ywa.1. human. [A2RUS2-3]

Organism-specific databases

CTD22898.
GeneCardsGC08P142156.
H-InvDBHIX0007822.
HGNCHGNC:29134. DENND3.
HPAHPA026100.
neXtProtNX_A2RUS2.
PharmGKBPA142671987.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG115977.
HOGENOMHOG000060114.
HOVERGENHBG095574.
InParanoidA2RUS2.
PhylomeDBA2RUS2.
TreeFamTF331814.

Enzyme and pathway databases

SignaLinkA2RUS2.

Gene expression databases

ArrayExpressA2RUS2.
BgeeA2RUS2.
CleanExHS_DENND3.
GenevestigatorA2RUS2.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi22898.
NextBio43523.
PROA2RUS2.

Entry information

Entry nameDEND3_HUMAN
AccessionPrimary (citable) accession number: A2RUS2
Secondary accession number(s): B7ZM28 expand/collapse secondary AC list , O94947, Q2TAM7, Q6ZMS6, Q96DK3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: July 9, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM