Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DENN domain-containing protein 3

Gene

DENND3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. Thereby, may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor.1 Publication

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.
SignaLinkiA2RUS2.

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 3
Gene namesi
Name:DENND3
Synonyms:KIAA0870
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:29134. DENND3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi22898.
OpenTargetsiENSG00000105339.
PharmGKBiPA142671987.

Polymorphism and mutation databases

BioMutaiDENND3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003046721 – 1198DENN domain-containing protein 3Add BLAST1198

Proteomic databases

MaxQBiA2RUS2.
PaxDbiA2RUS2.
PRIDEiA2RUS2.

PTM databases

iPTMnetiA2RUS2.
PhosphoSitePlusiA2RUS2.

Expressioni

Gene expression databases

BgeeiENSG00000105339.
CleanExiHS_DENND3.
ExpressionAtlasiA2RUS2. baseline and differential.
GenevisibleiA2RUS2. HS.

Organism-specific databases

HPAiHPA026100.

Interactioni

Protein-protein interaction databases

BioGridi116562. 1 interactor.
IntActiA2RUS2. 6 interactors.
STRINGi9606.ENSP00000262585.

Structurei

3D structure databases

ProteinModelPortaliA2RUS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 83UDENNAdd BLAST78
Domaini105 – 291DENNPROSITE-ProRule annotationAdd BLAST187
Domaini354 – 417dDENNPROSITE-ProRule annotationAdd BLAST64
Repeati652 – 719WD 1Add BLAST68
Repeati720 – 768WD 2Add BLAST49
Repeati769 – 814WD 3Add BLAST46
Repeati815 – 869WD 4Add BLAST55
Repeati870 – 935WD 5Add BLAST66
Repeati936 – 976WD 6Add BLAST41
Repeati977 – 1021WD 7Add BLAST45
Repeati1022 – 1064WD 8Add BLAST43
Repeati1065 – 1109WD 9Add BLAST45
Repeati1110 – 1157WD 10Add BLAST48
Repeati1158 – 1196WD 11Add BLAST39

Sequence similaritiesi

Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation
Contains 11 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IQM8. Eukaryota.
ENOG410XTT7. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000060114.
HOVERGENiHBG095574.
InParanoidiA2RUS2.
KOiK20162.
PhylomeDBiA2RUS2.
TreeFamiTF331814.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02141. DENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2RUS2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSLRKKREK PRPEQWKGLP GPPRAPEPED VAVPGGVDLL TLPQLCFPGG
60 70 80 90 100
VCVATEPKED CVHFLVLTDV CGNRTYGVVA QYYRPLHDEY CFYNGKTHRE
110 120 130 140 150
CPGCFVPFAV CVVSRFPYYN SLKDCLSCLL ALLKPCKDFE VDSHIKDFAA
160 170 180 190 200
KLSLIPSPPP GPLHLVFNMK SLQIVLPARA DPESPILDLD LHLPLLCFRP
210 220 230 240 250
EKVLQILTCI LTEQRIVFFS SDWALLTLVT ECFMAYLYPL QWQHPFVPIL
260 270 280 290 300
SDQMLDFVMA PTSFLMGCHL DHFEEVSKEA DGLVLINIDH GSITYSKSTD
310 320 330 340 350
DNVDIPDVPL LAAQTFIQRV QSLQLHHELH AAHLLSSTDL KEGRAHRRSW
360 370 380 390 400
QQKLNCQIQQ TTLQLLVSIF RDVKNHLNYE HRVFNSEEFL KTRAPGDHQF
410 420 430 440 450
YKQVLDTYMF HSFLKARLNR RMDAFAQMDL DTQSEEDRIN GMLLSPRRPT
460 470 480 490 500
VEKRASRKSS HLHVTHRRMV VSMPNLQDIA MPELAPRNSS LRLTDTAGCR
510 520 530 540 550
GSSAVLNVTP KSPYTFKIPE IHFPLESKCV QAYHAHFVSM LSEAMCFLAP
560 570 580 590 600
DNSLLLARYL YLRGLVYLMQ GQLLNALLDF QNLYKTDIRI FPTDLVKRTV
610 620 630 640 650
ESMSAPEWEG AEQAPELMRL ISEILDKPHE ASKLDDHVKK FKLPKKHMQL
660 670 680 690 700
GDFMKRVQES GIVKDASIIH RLFEALTVGQ EKQIDPETFK DFYNCWKETE
710 720 730 740 750
AEAQEVSLPW LVMEHLDKNE CVCKLSSSVK TNLGVGKIAM TQKRLFLLTE
760 770 780 790 800
GRPGYLEIST FRNIEEVRRT TTTFLLRRIP TLKIRVASKK EVFEANLKTE
810 820 830 840 850
CDLWHLMVKE MWAGKKLADD HKDPHYVQQA LTNVLLMDAV VGTLQSPGAI
860 870 880 890 900
YAASKLSYFD KMSNEMPMTL PETTLETLKH KINPSAGEAF PQAVDVLLYT
910 920 930 940 950
PGHLDPAEKV EDAHPKLWCA LSEGKVTVFN ASSWTIHQHS FKVGTAKVNC
960 970 980 990 1000
MVMADQNQVW VGSEDSVIYI INVHSMSCNK QLTAHCSSVT DLIVQDGQEA
1010 1020 1030 1040 1050
PSNVYSCSMD GMVLVWNVST LQVTSRFQLP RGGLTSIRLH GGRLWCCTGN
1060 1070 1080 1090 1100
SIMVMKMNGS LHQELKIEEN FKDTSTSFLA FQLLPEEEQL WAACAGRSEV
1110 1120 1130 1140 1150
YIWSLKDLAQ PPQRVPLEDC SEINCMIRVK KQVWVGSRGL GQGTPKGKIY
1160 1170 1180 1190
VIDAERKTVE KELVAHMDTV RTLCSAEDRY VLSGSGREEG KVAIWKGE
Length:1,198
Mass (Da):135,890
Last modified:September 11, 2007 - v2
Checksum:i52D51635DDD88A4E
GO
Isoform 2 (identifier: A2RUS2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-370: Missing.

Note: No experimental confirmation available.
Show »
Length:1,146
Mass (Da):129,809
Checksum:iB8D7B60A600DDD0C
GO
Isoform 3 (identifier: A2RUS2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-950: Missing.
     1133-1198: VWVGSRGLGQ...EGKVAIWKGE → VGWRARHPQH...PNWMASNDRT

Note: No experimental confirmation available.
Show »
Length:266
Mass (Da):29,486
Checksum:iB5D62298848A4332
GO
Isoform 4 (identifier: A2RUS2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     128-128: C → W
     129-1198: Missing.

Note: No experimental confirmation available.
Show »
Length:128
Mass (Da):14,509
Checksum:iB5E0DB642AC3F733
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035053143S → N.Corresponds to variant rs307761dbSNPEnsembl.1
Natural variantiVAR_035054364Q → R.Corresponds to variant rs11997191dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0280751 – 950Missing in isoform 3. 1 PublicationAdd BLAST950
Alternative sequenceiVSP_028077128C → W in isoform 4. 1 Publication1
Alternative sequenceiVSP_028078129 – 1198Missing in isoform 4. 1 PublicationAdd BLAST1070
Alternative sequenceiVSP_028080319 – 370Missing in isoform 2. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_0280811133 – 1198VWVGS…IWKGE → VGWRARHPQHPRQVSLALAA SPCSREPAARPRALLPSPLR VPLLTGTCRVGANGPTGHHV LVSWCGPSTPQRIPPNWMAS NDRT in isoform 3. 1 PublicationAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK058001 mRNA. Translation: BAB71630.1.
AK131505 mRNA. Translation: BAD18649.1.
AC040970 Genomic DNA. No translation available.
BC133023 mRNA. Translation: AAI33024.1.
BC144226 mRNA. Translation: AAI44227.1.
AB020677 mRNA. Translation: BAA74893.2.
CCDSiCCDS34947.1. [A2RUS2-1]
RefSeqiNP_055772.2. NM_014957.2. [A2RUS2-1]
UniGeneiHs.18166.

Genome annotation databases

EnsembliENST00000262585; ENSP00000262585; ENSG00000105339. [A2RUS2-1]
ENST00000424248; ENSP00000410594; ENSG00000105339. [A2RUS2-2]
GeneIDi22898.
KEGGihsa:22898.
UCSCiuc003yvy.4. human. [A2RUS2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK058001 mRNA. Translation: BAB71630.1.
AK131505 mRNA. Translation: BAD18649.1.
AC040970 Genomic DNA. No translation available.
BC133023 mRNA. Translation: AAI33024.1.
BC144226 mRNA. Translation: AAI44227.1.
AB020677 mRNA. Translation: BAA74893.2.
CCDSiCCDS34947.1. [A2RUS2-1]
RefSeqiNP_055772.2. NM_014957.2. [A2RUS2-1]
UniGeneiHs.18166.

3D structure databases

ProteinModelPortaliA2RUS2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116562. 1 interactor.
IntActiA2RUS2. 6 interactors.
STRINGi9606.ENSP00000262585.

PTM databases

iPTMnetiA2RUS2.
PhosphoSitePlusiA2RUS2.

Polymorphism and mutation databases

BioMutaiDENND3.

Proteomic databases

MaxQBiA2RUS2.
PaxDbiA2RUS2.
PRIDEiA2RUS2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262585; ENSP00000262585; ENSG00000105339. [A2RUS2-1]
ENST00000424248; ENSP00000410594; ENSG00000105339. [A2RUS2-2]
GeneIDi22898.
KEGGihsa:22898.
UCSCiuc003yvy.4. human. [A2RUS2-1]

Organism-specific databases

CTDi22898.
DisGeNETi22898.
GeneCardsiDENND3.
H-InvDBHIX0007822.
HGNCiHGNC:29134. DENND3.
HPAiHPA026100.
neXtProtiNX_A2RUS2.
OpenTargetsiENSG00000105339.
PharmGKBiPA142671987.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQM8. Eukaryota.
ENOG410XTT7. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000060114.
HOVERGENiHBG095574.
InParanoidiA2RUS2.
KOiK20162.
PhylomeDBiA2RUS2.
TreeFamiTF331814.

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.
SignaLinkiA2RUS2.

Miscellaneous databases

ChiTaRSiDENND3. human.
GenomeRNAii22898.
PROiA2RUS2.

Gene expression databases

BgeeiENSG00000105339.
CleanExiHS_DENND3.
ExpressionAtlasiA2RUS2. baseline and differential.
GenevisibleiA2RUS2. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02141. DENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEND3_HUMAN
AccessioniPrimary (citable) accession number: A2RUS2
Secondary accession number(s): B7ZM28
, O94947, Q2TAM7, Q6ZMS6, Q96DK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 30, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.