Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A2RU49 (HYKK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxylysine kinase

Short name=5-hydroxy-L-lysine kinase
EC=2.7.1.81
Alternative name(s):
Aminoglycoside phosphotransferase domain-containing protein 1
Gene names
Name:HYKK
Synonyms:AGPHD1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine. Ref.5

Catalytic activity

GTP + 5-hydroxy-L-lysine = GDP + 5-phosphonooxy-L-lysine. Ref.5

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the aminoglycoside phosphotransferase family.

Biophysicochemical properties

Kinetic parameters:

KM=280 µM for (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP) Ref.5

KM=7.6 µM for (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP)

KM=810 µM for ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)

KM=3.5 µM for GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)

Vmax=4.95 µmol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP)

Vmax=0.49 µmol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP)

Vmax=5.28 µmol/min/mg enzyme toward ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)

Vmax=0.57 µmol/min/mg enzyme toward GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhydroxylysine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A2RU49-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A2RU49-2)

The sequence of this isoform differs from the canonical sequence as follows:
     160-172: RFHHPKLSSLHRE → EGKPRVTPLLAKN
     173-373: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: A2RU49-3)

The sequence of this isoform differs from the canonical sequence as follows:
     222-226: INHGD → EYSPN
     227-373: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Hydroxylysine kinase
PRO_0000326044

Sites

Active site2261Proton acceptor By similarity

Natural variations

Alternative sequence160 – 17213RFHHP…SLHRE → EGKPRVTPLLAKN in isoform 2.
VSP_032517
Alternative sequence173 – 373201Missing in isoform 2.
VSP_032518
Alternative sequence222 – 2265INHGD → EYSPN in isoform 3.
VSP_032519
Alternative sequence227 – 373147Missing in isoform 3.
VSP_032520
Isoform 2:
Natural variant1651V → I in dbSNP:4380026.

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: C8BF3521D8D980A2

FASTA37341,933
        10         20         30         40         50         60 
MSSGNYQQSE ALSKPTFSEE QASALVESVF GLKVSKVRPL PSYDDQNFHV YVSKTKDGPT 

        70         80         90        100        110        120 
EYVLKISNTK ASKNPDLIEV QNHIIMFLKA AGFPTASVCH TKGDNTASLV SVDSGSEIKS 

       130        140        150        160        170        180 
YLVRLLTYLP GRPIAELPVS PQLLYEIGKL AAKLDKTLQR FHHPKLSSLH RENFIWNLKN 

       190        200        210        220        230        240 
VPLLEKYLYA LGQNRNREIV EHVIHLFKEE VMTKLSHFRE CINHGDLNDH NILIESSKSA 

       250        260        270        280        290        300 
SGNAEYQVSG ILDFGDMSYG YYVFEVAITI MYMMIESKSP IQVGGHVLAG FESITPLTAV 

       310        320        330        340        350        360 
EKGALFLLVC SRFCQSLVMA AYSCQLYPEN KDYLMVTAKT GWKHLQQMFD MGQKAVEEIW 

       370 
FETAKSYESG ISM 

« Hide

Isoform 2 [UniParc].

Checksum: F052756328B9A2A0
Show »

FASTA17218,782
Isoform 3 [UniParc].

Checksum: EAE7FA53D9AFE7A8
Show »

FASTA22625,589

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Uterus.
[2]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Brain.
[5]"Molecular identification of hydroxylysine kinase and of ammoniophospholyases acting on 5-phosphohydroxy-L-lysine and phosphoethanolamine."
Veiga-da-Cunha M., Hadi F., Balligand T., Stroobant V., Van Schaftingen E.
J. Biol. Chem. 287:7246-7255(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK126453 mRNA. Translation: BAC86556.1.
AC027228 Genomic DNA. No translation available.
CH471136 Genomic DNA. Translation: EAW99167.1.
BC132753 mRNA. Translation: AAI32754.1.
BC144383 mRNA. Translation: AAI44384.1.
RefSeqNP_001013641.2. NM_001013619.2.
NP_001077081.1. NM_001083612.1.
UniGeneHs.307962.

3D structure databases

ProteinModelPortalA2RU49.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125829. 1 interaction.
STRING9606.ENSP00000373640.

PTM databases

PhosphoSiteA2RU49.

Proteomic databases

PaxDbA2RU49.
PRIDEA2RU49.

Protocols and materials databases

DNASU123688.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000360519; ENSP00000353710; ENSG00000188266. [A2RU49-2]
ENST00000388988; ENSP00000373640; ENSG00000188266. [A2RU49-1]
ENST00000566289; ENSP00000456614; ENSG00000188266. [A2RU49-2]
ENST00000566332; ENSP00000457154; ENSG00000188266. [A2RU49-3]
ENST00000569878; ENSP00000455459; ENSG00000188266. [A2RU49-1]
GeneID123688.
KEGGhsa:123688.
UCSCuc002bdt.3. human. [A2RU49-3]
uc010ble.3. human. [A2RU49-1]

Organism-specific databases

CTD123688.
GeneCardsGC15P078800.
HGNCHGNC:34403. HYKK.
HPAHPA040706.
MIM614681. gene.
neXtProtNX_A2RU49.
PharmGKBPA165478483.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2334.
HOGENOMHOG000034013.
InParanoidA2RU49.
OMAETAKSYE.
OrthoDBEOG77WWC8.
PhylomeDBA2RU49.
TreeFamTF324471.

Gene expression databases

ArrayExpressA2RU49.
BgeeA2RU49.
GenevestigatorA2RU49.

Family and domain databases

InterProIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamPF01636. APH. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi123688.
NextBio81124.
PROA2RU49.
SOURCESearch...

Entry information

Entry nameHYKK_HUMAN
AccessionPrimary (citable) accession number: A2RU49
Secondary accession number(s): B7ZMA5, Q6ZTN0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: March 19, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM