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Protein

Hydroxylysine kinase

Gene

HYKK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine.1 Publication

Catalytic activityi

GTP + 5-hydroxy-L-lysine = GDP + 5-phosphonooxy-L-lysine.1 Publication

Kineticsi

  1. KM=280 µM for (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP)1 Publication
  2. KM=7.6 µM for (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP)1 Publication
  3. KM=810 µM for ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)1 Publication
  4. KM=3.5 µM for GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)1 Publication

Vmax=4.95 µmol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP)1 Publication

Vmax=0.49 µmol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP)1 Publication

Vmax=5.28 µmol/min/mg enzyme toward ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)1 Publication

Vmax=0.57 µmol/min/mg enzyme toward GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei226 – 2261Proton acceptorBy similarity

GO - Molecular functioni

  1. hydroxylysine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Enzyme and pathway databases

BRENDAi2.7.1.81. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxylysine kinase (EC:2.7.1.81)
Short name:
5-hydroxy-L-lysine kinase
Alternative name(s):
Aminoglycoside phosphotransferase domain-containing protein 1
Gene namesi
Name:HYKK
Synonyms:AGPHD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:34403. HYKK.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165478483.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Hydroxylysine kinasePRO_0000326044Add
BLAST

Proteomic databases

MaxQBiA2RU49.
PaxDbiA2RU49.
PRIDEiA2RU49.

PTM databases

PhosphoSiteiA2RU49.

Expressioni

Gene expression databases

BgeeiA2RU49.
GenevestigatoriA2RU49.

Organism-specific databases

HPAiHPA040706.

Interactioni

Protein-protein interaction databases

BioGridi125829. 4 interactions.
STRINGi9606.ENSP00000373640.

Structurei

3D structure databases

ProteinModelPortaliA2RU49.
SMRiA2RU49. Positions 220-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2334.
GeneTreeiENSGT00390000011314.
HOGENOMiHOG000034013.
InParanoidiA2RU49.
KOiK18201.
OMAiFKEEVMT.
OrthoDBiEOG77WWC8.
PhylomeDBiA2RU49.
TreeFamiTF324471.

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2RU49-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSGNYQQSE ALSKPTFSEE QASALVESVF GLKVSKVRPL PSYDDQNFHV
60 70 80 90 100
YVSKTKDGPT EYVLKISNTK ASKNPDLIEV QNHIIMFLKA AGFPTASVCH
110 120 130 140 150
TKGDNTASLV SVDSGSEIKS YLVRLLTYLP GRPIAELPVS PQLLYEIGKL
160 170 180 190 200
AAKLDKTLQR FHHPKLSSLH RENFIWNLKN VPLLEKYLYA LGQNRNREIV
210 220 230 240 250
EHVIHLFKEE VMTKLSHFRE CINHGDLNDH NILIESSKSA SGNAEYQVSG
260 270 280 290 300
ILDFGDMSYG YYVFEVAITI MYMMIESKSP IQVGGHVLAG FESITPLTAV
310 320 330 340 350
EKGALFLLVC SRFCQSLVMA AYSCQLYPEN KDYLMVTAKT GWKHLQQMFD
360 370
MGQKAVEEIW FETAKSYESG ISM
Length:373
Mass (Da):41,933
Last modified:March 18, 2008 - v2
Checksum:iC8BF3521D8D980A2
GO
Isoform 2 (identifier: A2RU49-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-172: RFHHPKLSSLHRE → EGKPRVTPLLAKN
     173-373: Missing.

Note: No experimental confirmation available.

Show »
Length:172
Mass (Da):18,782
Checksum:iF052756328B9A2A0
GO
Isoform 3 (identifier: A2RU49-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     221-373: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.

Show »
Length:220
Mass (Da):24,896
Checksum:i17A8E9E366636EFE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: A2RU49-2)
Natural varianti165 – 1651V → I in dbSNP:4380026.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei160 – 17213RFHHP…SLHRE → EGKPRVTPLLAKN in isoform 2. 1 PublicationVSP_032517Add
BLAST
Alternative sequencei173 – 373201Missing in isoform 2. 1 PublicationVSP_032518Add
BLAST
Alternative sequencei221 – 373153Missing in isoform 3. 1 PublicationVSP_032520Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK126453 mRNA. Translation: BAC86556.1.
AC027228 Genomic DNA. No translation available.
CH471136 Genomic DNA. Translation: EAW99167.1.
BC132753 mRNA. Translation: AAI32754.1.
BC144383 mRNA. Translation: AAI44384.1.
CCDSiCCDS42063.1. [A2RU49-1]
CCDS45318.1. [A2RU49-3]
RefSeqiNP_001013641.2. NM_001013619.2. [A2RU49-1]
NP_001077081.1. NM_001083612.1. [A2RU49-3]
UniGeneiHs.307962.

Genome annotation databases

EnsembliENST00000388988; ENSP00000373640; ENSG00000188266. [A2RU49-1]
ENST00000408962; ENSP00000386197; ENSG00000188266. [A2RU49-3]
ENST00000563233; ENSP00000454850; ENSG00000188266. [A2RU49-3]
ENST00000566289; ENSP00000456614; ENSG00000188266. [A2RU49-2]
ENST00000569878; ENSP00000455459; ENSG00000188266. [A2RU49-1]
GeneIDi123688.
KEGGihsa:123688.
UCSCiuc002bdt.3. human. [A2RU49-3]
uc010ble.3. human. [A2RU49-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK126453 mRNA. Translation: BAC86556.1.
AC027228 Genomic DNA. No translation available.
CH471136 Genomic DNA. Translation: EAW99167.1.
BC132753 mRNA. Translation: AAI32754.1.
BC144383 mRNA. Translation: AAI44384.1.
CCDSiCCDS42063.1. [A2RU49-1]
CCDS45318.1. [A2RU49-3]
RefSeqiNP_001013641.2. NM_001013619.2. [A2RU49-1]
NP_001077081.1. NM_001083612.1. [A2RU49-3]
UniGeneiHs.307962.

3D structure databases

ProteinModelPortaliA2RU49.
SMRiA2RU49. Positions 220-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125829. 4 interactions.
STRINGi9606.ENSP00000373640.

PTM databases

PhosphoSiteiA2RU49.

Proteomic databases

MaxQBiA2RU49.
PaxDbiA2RU49.
PRIDEiA2RU49.

Protocols and materials databases

DNASUi123688.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000388988; ENSP00000373640; ENSG00000188266. [A2RU49-1]
ENST00000408962; ENSP00000386197; ENSG00000188266. [A2RU49-3]
ENST00000563233; ENSP00000454850; ENSG00000188266. [A2RU49-3]
ENST00000566289; ENSP00000456614; ENSG00000188266. [A2RU49-2]
ENST00000569878; ENSP00000455459; ENSG00000188266. [A2RU49-1]
GeneIDi123688.
KEGGihsa:123688.
UCSCiuc002bdt.3. human. [A2RU49-3]
uc010ble.3. human. [A2RU49-1]

Organism-specific databases

CTDi123688.
GeneCardsiGC15P078800.
HGNCiHGNC:34403. HYKK.
HPAiHPA040706.
MIMi614681. gene.
neXtProtiNX_A2RU49.
PharmGKBiPA165478483.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2334.
GeneTreeiENSGT00390000011314.
HOGENOMiHOG000034013.
InParanoidiA2RU49.
KOiK18201.
OMAiFKEEVMT.
OrthoDBiEOG77WWC8.
PhylomeDBiA2RU49.
TreeFamiTF324471.

Enzyme and pathway databases

BRENDAi2.7.1.81. 2681.

Miscellaneous databases

GenomeRNAii123688.
NextBioi81124.
PROiA2RU49.
SOURCEiSearch...

Gene expression databases

BgeeiA2RU49.
GenevestigatoriA2RU49.

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Uterus.
  2. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain.
  5. "Molecular identification of hydroxylysine kinase and of ammoniophospholyases acting on 5-phosphohydroxy-L-lysine and phosphoethanolamine."
    Veiga-da-Cunha M., Hadi F., Balligand T., Stroobant V., Van Schaftingen E.
    J. Biol. Chem. 287:7246-7255(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiHYKK_HUMAN
AccessioniPrimary (citable) accession number: A2RU49
Secondary accession number(s): B7ZMA5, F8W6X5, Q6ZTN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: April 1, 2015
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.