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A2RU49

- HYKK_HUMAN

UniProt

A2RU49 - HYKK_HUMAN

Protein

Hydroxylysine kinase

Gene

HYKK

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine.1 Publication

    Catalytic activityi

    GTP + 5-hydroxy-L-lysine = GDP + 5-phosphonooxy-L-lysine.1 Publication

    Kineticsi

    1. KM=280 µM for (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP)1 Publication
    2. KM=7.6 µM for (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP)1 Publication
    3. KM=810 µM for ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)1 Publication
    4. KM=3.5 µM for GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)1 Publication

    Vmax=4.95 µmol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP)1 Publication

    Vmax=0.49 µmol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP)1 Publication

    Vmax=5.28 µmol/min/mg enzyme toward ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)1 Publication

    Vmax=0.57 µmol/min/mg enzyme toward GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei226 – 2261Proton acceptorBy similarity

    GO - Molecular functioni

    1. hydroxylysine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxylysine kinase (EC:2.7.1.81)
    Short name:
    5-hydroxy-L-lysine kinase
    Alternative name(s):
    Aminoglycoside phosphotransferase domain-containing protein 1
    Gene namesi
    Name:HYKK
    Synonyms:AGPHD1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:34403. HYKK.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA165478483.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373Hydroxylysine kinasePRO_0000326044Add
    BLAST

    Proteomic databases

    PaxDbiA2RU49.
    PRIDEiA2RU49.

    PTM databases

    PhosphoSiteiA2RU49.

    Expressioni

    Gene expression databases

    ArrayExpressiA2RU49.
    BgeeiA2RU49.
    GenevestigatoriA2RU49.

    Organism-specific databases

    HPAiHPA040706.

    Interactioni

    Protein-protein interaction databases

    BioGridi125829. 1 interaction.
    STRINGi9606.ENSP00000373640.

    Structurei

    3D structure databases

    ProteinModelPortaliA2RU49.
    SMRiA2RU49. Positions 220-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2334.
    HOGENOMiHOG000034013.
    InParanoidiA2RU49.
    KOiK18201.
    OMAiETAKSYE.
    OrthoDBiEOG77WWC8.
    PhylomeDBiA2RU49.
    TreeFamiTF324471.

    Family and domain databases

    InterProiIPR002575. Aminoglycoside_PTrfase.
    IPR011009. Kinase-like_dom.
    [Graphical view]
    PfamiPF01636. APH. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A2RU49-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSGNYQQSE ALSKPTFSEE QASALVESVF GLKVSKVRPL PSYDDQNFHV    50
    YVSKTKDGPT EYVLKISNTK ASKNPDLIEV QNHIIMFLKA AGFPTASVCH 100
    TKGDNTASLV SVDSGSEIKS YLVRLLTYLP GRPIAELPVS PQLLYEIGKL 150
    AAKLDKTLQR FHHPKLSSLH RENFIWNLKN VPLLEKYLYA LGQNRNREIV 200
    EHVIHLFKEE VMTKLSHFRE CINHGDLNDH NILIESSKSA SGNAEYQVSG 250
    ILDFGDMSYG YYVFEVAITI MYMMIESKSP IQVGGHVLAG FESITPLTAV 300
    EKGALFLLVC SRFCQSLVMA AYSCQLYPEN KDYLMVTAKT GWKHLQQMFD 350
    MGQKAVEEIW FETAKSYESG ISM 373
    Length:373
    Mass (Da):41,933
    Last modified:March 18, 2008 - v2
    Checksum:iC8BF3521D8D980A2
    GO
    Isoform 2 (identifier: A2RU49-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         160-172: RFHHPKLSSLHRE → EGKPRVTPLLAKN
         173-373: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:172
    Mass (Da):18,782
    Checksum:iF052756328B9A2A0
    GO
    Isoform 3 (identifier: A2RU49-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         221-373: Missing.

    Note: No experimental confirmation available. Gene prediction based on EST data.

    Show »
    Length:220
    Mass (Da):24,896
    Checksum:i17A8E9E366636EFE
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Isoform 2 (identifier: A2RU49-2)
    Natural varianti165 – 1651V → I in dbSNP:4380026.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei160 – 17213RFHHP…SLHRE → EGKPRVTPLLAKN in isoform 2. 1 PublicationVSP_032517Add
    BLAST
    Alternative sequencei173 – 373201Missing in isoform 2. 1 PublicationVSP_032518Add
    BLAST
    Alternative sequencei221 – 373153Missing in isoform 3. 1 PublicationVSP_032520Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK126453 mRNA. Translation: BAC86556.1.
    AC027228 Genomic DNA. No translation available.
    CH471136 Genomic DNA. Translation: EAW99167.1.
    BC132753 mRNA. Translation: AAI32754.1.
    BC144383 mRNA. Translation: AAI44384.1.
    CCDSiCCDS42063.1. [A2RU49-1]
    CCDS45318.1. [A2RU49-3]
    RefSeqiNP_001013641.2. NM_001013619.2. [A2RU49-1]
    NP_001077081.1. NM_001083612.1. [A2RU49-3]
    UniGeneiHs.307962.

    Genome annotation databases

    EnsembliENST00000388988; ENSP00000373640; ENSG00000188266. [A2RU49-1]
    ENST00000408962; ENSP00000386197; ENSG00000188266. [A2RU49-3]
    ENST00000563233; ENSP00000454850; ENSG00000188266. [A2RU49-3]
    ENST00000566289; ENSP00000456614; ENSG00000188266. [A2RU49-2]
    ENST00000566332; ENSP00000457154; ENSG00000188266. [A2RU49-3]
    ENST00000569878; ENSP00000455459; ENSG00000188266. [A2RU49-1]
    GeneIDi123688.
    KEGGihsa:123688.
    UCSCiuc002bdt.3. human. [A2RU49-3]
    uc010ble.3. human. [A2RU49-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK126453 mRNA. Translation: BAC86556.1 .
    AC027228 Genomic DNA. No translation available.
    CH471136 Genomic DNA. Translation: EAW99167.1 .
    BC132753 mRNA. Translation: AAI32754.1 .
    BC144383 mRNA. Translation: AAI44384.1 .
    CCDSi CCDS42063.1. [A2RU49-1 ]
    CCDS45318.1. [A2RU49-3 ]
    RefSeqi NP_001013641.2. NM_001013619.2. [A2RU49-1 ]
    NP_001077081.1. NM_001083612.1. [A2RU49-3 ]
    UniGenei Hs.307962.

    3D structure databases

    ProteinModelPortali A2RU49.
    SMRi A2RU49. Positions 220-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 125829. 1 interaction.
    STRINGi 9606.ENSP00000373640.

    PTM databases

    PhosphoSitei A2RU49.

    Proteomic databases

    PaxDbi A2RU49.
    PRIDEi A2RU49.

    Protocols and materials databases

    DNASUi 123688.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000388988 ; ENSP00000373640 ; ENSG00000188266 . [A2RU49-1 ]
    ENST00000408962 ; ENSP00000386197 ; ENSG00000188266 . [A2RU49-3 ]
    ENST00000563233 ; ENSP00000454850 ; ENSG00000188266 . [A2RU49-3 ]
    ENST00000566289 ; ENSP00000456614 ; ENSG00000188266 . [A2RU49-2 ]
    ENST00000566332 ; ENSP00000457154 ; ENSG00000188266 . [A2RU49-3 ]
    ENST00000569878 ; ENSP00000455459 ; ENSG00000188266 . [A2RU49-1 ]
    GeneIDi 123688.
    KEGGi hsa:123688.
    UCSCi uc002bdt.3. human. [A2RU49-3 ]
    uc010ble.3. human. [A2RU49-1 ]

    Organism-specific databases

    CTDi 123688.
    GeneCardsi GC15P078800.
    HGNCi HGNC:34403. HYKK.
    HPAi HPA040706.
    MIMi 614681. gene.
    neXtProti NX_A2RU49.
    PharmGKBi PA165478483.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2334.
    HOGENOMi HOG000034013.
    InParanoidi A2RU49.
    KOi K18201.
    OMAi ETAKSYE.
    OrthoDBi EOG77WWC8.
    PhylomeDBi A2RU49.
    TreeFami TF324471.

    Miscellaneous databases

    GenomeRNAii 123688.
    NextBioi 81124.
    PROi A2RU49.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A2RU49.
    Bgeei A2RU49.
    Genevestigatori A2RU49.

    Family and domain databases

    InterProi IPR002575. Aminoglycoside_PTrfase.
    IPR011009. Kinase-like_dom.
    [Graphical view ]
    Pfami PF01636. APH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Uterus.
    2. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Brain.
    5. "Molecular identification of hydroxylysine kinase and of ammoniophospholyases acting on 5-phosphohydroxy-L-lysine and phosphoethanolamine."
      Veiga-da-Cunha M., Hadi F., Balligand T., Stroobant V., Van Schaftingen E.
      J. Biol. Chem. 287:7246-7255(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiHYKK_HUMAN
    AccessioniPrimary (citable) accession number: A2RU49
    Secondary accession number(s): B7ZMA5, F8W6X5, Q6ZTN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3