A2RTX5 (SYTC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable threonine--tRNA ligase 2, cytoplasmic
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS
Threonyl-tRNA synthetase-like protein 2

Gene names

Name:

TARSL2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	802 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence BAB71554.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A2RTX5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A2RTX5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     225-319: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 802	801	Probable threonine--tRNA ligase 2, cytoplasmic		PRO_0000333828
Regions
	Coiled coil	3 – 23	21	Potential
	Coiled coil	76 – 96	21	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.4
Natural variations
	Alternative sequence	225 – 319	95	Missing in isoform 2.		VSP_033562
Experimental info
	Sequence conflict	96	1	A → G in BAB71241. Ref.1
	Sequence conflict	166	1	V → A in BAB71241. Ref.1
	Sequence conflict	210	1	R → C in BAB71241. Ref.1
	Sequence conflict	337	1	I → N in AAH69346. Ref.3
	Sequence conflict	337	1	I → N in AAH69811. Ref.3
	Sequence conflict	513	1	F → C in BAB71554. Ref.1
	Sequence conflict	533	1	R → G in BAB71554. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: EE83EB934B503E59	FASTA	802	92,646
	10 20 30 40 50 60 MAAEALAAEA VASRLERQEE DIRWLWSEVE RLRDEQLNAP YSCQAEGPCL TREVAQLRAE 70 80 90 100 110 120 NCDLRHRLCS LRLCLAEERS RQATLESAEL EAAQEAGAQP PPSQSQDKDM KKKKMKESEA 130 140 150 160 170 180 DSEVKHQPIF IKERLKLFEI LKKDHQLLLA IYGKKGDTSN IITVRVADGQ TVQGEVWKTT 190 200 210 220 230 240 PYQVAAEISQ ELAESTVIAK VNGELWDLDR PLEGDSSLEL LTFDNEEAQA VYWHSSAHIL 250 260 270 280 290 300 GEAMELYYGG HLCYGPPIEN GFYYDMFIED RAVSSTELSA LENICKAIIK EKQPFERLEV 310 320 330 340 350 360 SKEILLEMFK YNKFKCRILN EKVNTATTTV YRCGPLIDLC KGPHVRHTGK IKTIKIFKNS 370 380 390 400 410 420 STYWEGNPEM ETLQRIYGIS FPDNKMMRDW EKFQEEAKNR DHRKIGKEQE LFFFHDLSPG 430 440 450 460 470 480 SCFFLPRGAF IYNTLTDFIR EEYHKRDFTE VLSPNMYNSK LWEASGHWQH YSENMFTFEI 490 500 510 520 530 540 EKDTFALKPM NCPGHCLMFA HRPRSWREMP IRFADFGVLH RNELSGTLSG LTRVRRFQQD 550 560 570 580 590 600 DAHIFCTVEQ IEEEIKGCLQ FLQSVYSTFG FSFQLNLSTR PENFLGEIEM WNEAEKQLQN 610 620 630 640 650 660 SLMDFGEPWK MNPGDGAFYG PKIDIKIKDA IGRYHQCATI QLDFQLPIRF NLTYVSKDGD 670 680 690 700 710 720 DKKRPVIIHR AILGSVERMI AILSENYGGK WPFWLSPRQV MVIPVGPTCE KYALQVSSEF 730 740 750 760 770 780 FEEGFMADVD LDHSCTLNKK IRNAQLAQYN FILVVGEKEK IDNAVNVRTR DNKIHGEILV 790 800 TSAIDKLKNL RKTRTLNAEE AF
	Isoform 2 [UniParc]. Checksum: 060179AFC7232B3B	FASTA	707	81,561
	10 20 30 40 50 60 MAAEALAAEA VASRLERQEE DIRWLWSEVE RLRDEQLNAP YSCQAEGPCL TREVAQLRAE 70 80 90 100 110 120 NCDLRHRLCS LRLCLAEERS RQATLESAEL EAAQEAGAQP PPSQSQDKDM KKKKMKESEA 130 140 150 160 170 180 DSEVKHQPIF IKERLKLFEI LKKDHQLLLA IYGKKGDTSN IITVRVADGQ TVQGEVWKTT 190 200 210 220 230 240 PYQVAAEISQ ELAESTVIAK VNGELWDLDR PLEGDSSLEL LTFDNEKVNT ATTTVYRCGP 250 260 270 280 290 300 LIDLCKGPHV RHTGKIKTIK IFKNSSTYWE GNPEMETLQR IYGISFPDNK MMRDWEKFQE 310 320 330 340 350 360 EAKNRDHRKI GKEQELFFFH DLSPGSCFFL PRGAFIYNTL TDFIREEYHK RDFTEVLSPN 370 380 390 400 410 420 MYNSKLWEAS GHWQHYSENM FTFEIEKDTF ALKPMNCPGH CLMFAHRPRS WREMPIRFAD 430 440 450 460 470 480 FGVLHRNELS GTLSGLTRVR RFQQDDAHIF CTVEQIEEEI KGCLQFLQSV YSTFGFSFQL 490 500 510 520 530 540 NLSTRPENFL GEIEMWNEAE KQLQNSLMDF GEPWKMNPGD GAFYGPKIDI KIKDAIGRYH 550 560 570 580 590 600 QCATIQLDFQ LPIRFNLTYV SKDGDDKKRP VIIHRAILGS VERMIAILSE NYGGKWPFWL 610 620 630 640 650 660 SPRQVMVIPV GPTCEKYALQ VSSEFFEEGF MADVDLDHSC TLNKKIRNAQ LAQYNFILVV 670 680 690 700 GEKEKIDNAV NVRTRDNKIH GEILVTSAID KLKNLRKTRT LNAEEAF

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 315-802 (ISOFORM 1). Tissue: Cerebellum.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 337-802 (ISOFORMS 1/2). Tissue: Brain and Lung.
[4]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK056653 mRNA. Translation: BAB71241.1. AK057734 mRNA. Translation: BAB71554.1. Different initiation. CH471101 Genomic DNA. Translation: EAX02316.1. BC069346 mRNA. Translation: AAH69346.1. BC069811 mRNA. Translation: AAH69811.1. BC074887 mRNA. Translation: AAH74887.1. BC132671 mRNA. Translation: AAI32672.1. BC136315 mRNA. Translation: AAI36316.1.
IPI	IPI00328082. IPI00892711.
RefSeq	NP_689547.2. NM_152334.2.
UniGene	Hs.657163.
3D structure databases
ProteinModelPortal	A2RTX5.
SMR	A2RTX5. Positions 161-801.
ModBase	Search...
Protein-protein interaction databases
STRING	A2RTX5.
PTM databases
PhosphoSite	A2RTX5.
Proteomic databases
PRIDE	A2RTX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335968; ENSP00000338093; ENSG00000185418.
GeneID	123283.
KEGG	hsa:123283.
NMPDR	fig|9606.3.peg.11324.
UCSC	uc002bxm.1. human.
Organism-specific databases
CTD	123283.
GeneCards	GC15M102193.
HGNC	HGNC:24728. TARSL2.
neXtProt	NX_A2RTX5.
PharmGKB	PA128394753.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04317.
HOGENOM	HBG352811.
HOVERGEN	HBG059513.
InParanoid	A2RTX5.
OMA	EEYAGKF.
OrthoDB	EOG4DBTD5.
PhylomeDB	A2RTX5.
Gene expression databases
Bgee	A2RTX5.
CleanEx	HS_TARSL2.
Genevestigator	A2RTX5.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	81100.

Entry information

Entry name

SYTC2_HUMAN

Accession

Primary (citable) accession number: A2RTX5
Secondary accession number(s): B2RMP7 , Q6B0A1, Q6IS76, Q96LW3, Q96MP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 6, 2007
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.