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Protein

Probable threonine--tRNA ligase 2, cytoplasmic

Gene

TARSL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. threonine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. threonyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable threonine--tRNA ligase 2, cytoplasmic (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Threonyl-tRNA synthetase-like protein 2
Gene namesi
Name:TARSL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:24728. TARSL2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394753.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 802801Probable threonine--tRNA ligase 2, cytoplasmicPRO_0000333828Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiA2RTX5.
PaxDbiA2RTX5.
PRIDEiA2RTX5.

PTM databases

PhosphoSiteiA2RTX5.

Expressioni

Gene expression databases

BgeeiA2RTX5.
CleanExiHS_TARSL2.
ExpressionAtlasiA2RTX5. baseline and differential.
GenevestigatoriA2RTX5.

Organism-specific databases

HPAiHPA063963.

Interactioni

Protein-protein interaction databases

BioGridi125822. 7 interactions.
STRINGi9606.ENSP00000338093.

Structurei

3D structure databases

ProteinModelPortaliA2RTX5.
SMRiA2RTX5. Positions 162-800.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili3 – 2321Sequence AnalysisAdd
BLAST
Coiled coili76 – 9621Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0441.
GeneTreeiENSGT00390000002149.
HOGENOMiHOG000003878.
HOVERGENiHBG059513.
InParanoidiA2RTX5.
KOiK01868.
OMAiKSGHWDK.
OrthoDBiEOG7JDQX3.
PhylomeDBiA2RTX5.
TreeFamiTF300858.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2RTX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAEALAAEA VASRLERQEE DIRWLWSEVE RLRDEQLNAP YSCQAEGPCL
60 70 80 90 100
TREVAQLRAE NCDLRHRLCS LRLCLAEERS RQATLESAEL EAAQEAGAQP
110 120 130 140 150
PPSQSQDKDM KKKKMKESEA DSEVKHQPIF IKERLKLFEI LKKDHQLLLA
160 170 180 190 200
IYGKKGDTSN IITVRVADGQ TVQGEVWKTT PYQVAAEISQ ELAESTVIAK
210 220 230 240 250
VNGELWDLDR PLEGDSSLEL LTFDNEEAQA VYWHSSAHIL GEAMELYYGG
260 270 280 290 300
HLCYGPPIEN GFYYDMFIED RAVSSTELSA LENICKAIIK EKQPFERLEV
310 320 330 340 350
SKEILLEMFK YNKFKCRILN EKVNTATTTV YRCGPLIDLC KGPHVRHTGK
360 370 380 390 400
IKTIKIFKNS STYWEGNPEM ETLQRIYGIS FPDNKMMRDW EKFQEEAKNR
410 420 430 440 450
DHRKIGKEQE LFFFHDLSPG SCFFLPRGAF IYNTLTDFIR EEYHKRDFTE
460 470 480 490 500
VLSPNMYNSK LWEASGHWQH YSENMFTFEI EKDTFALKPM NCPGHCLMFA
510 520 530 540 550
HRPRSWREMP IRFADFGVLH RNELSGTLSG LTRVRRFQQD DAHIFCTVEQ
560 570 580 590 600
IEEEIKGCLQ FLQSVYSTFG FSFQLNLSTR PENFLGEIEM WNEAEKQLQN
610 620 630 640 650
SLMDFGEPWK MNPGDGAFYG PKIDIKIKDA IGRYHQCATI QLDFQLPIRF
660 670 680 690 700
NLTYVSKDGD DKKRPVIIHR AILGSVERMI AILSENYGGK WPFWLSPRQV
710 720 730 740 750
MVIPVGPTCE KYALQVSSEF FEEGFMADVD LDHSCTLNKK IRNAQLAQYN
760 770 780 790 800
FILVVGEKEK IDNAVNVRTR DNKIHGEILV TSAIDKLKNL RKTRTLNAEE

AF
Length:802
Mass (Da):92,646
Last modified:March 6, 2007 - v1
Checksum:iEE83EB934B503E59
GO
Isoform 2 (identifier: A2RTX5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-319: Missing.

Show »
Length:707
Mass (Da):81,561
Checksum:i060179AFC7232B3B
GO

Sequence cautioni

The sequence BAB71554.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961A → G in BAB71241 (PubMed:14702039).Curated
Sequence conflicti166 – 1661V → A in BAB71241 (PubMed:14702039).Curated
Sequence conflicti210 – 2101R → C in BAB71241 (PubMed:14702039).Curated
Sequence conflicti337 – 3371I → N in AAH69346 (PubMed:15489334).Curated
Sequence conflicti337 – 3371I → N in AAH69811 (PubMed:15489334).Curated
Sequence conflicti513 – 5131F → C in BAB71554 (PubMed:14702039).Curated
Sequence conflicti533 – 5331R → G in BAB71554 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei225 – 31995Missing in isoform 2. 1 PublicationVSP_033562Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056653 mRNA. Translation: BAB71241.1.
AK057734 mRNA. Translation: BAB71554.1. Different initiation.
CH471101 Genomic DNA. Translation: EAX02316.1.
BC069346 mRNA. Translation: AAH69346.1.
BC069811 mRNA. Translation: AAH69811.1.
BC074887 mRNA. Translation: AAH74887.1.
BC132671 mRNA. Translation: AAI32672.1.
BC136315 mRNA. Translation: AAI36316.1.
CCDSiCCDS10394.1. [A2RTX5-1]
RefSeqiNP_689547.2. NM_152334.2. [A2RTX5-1]
UniGeneiHs.657163.

Genome annotation databases

EnsembliENST00000335968; ENSP00000338093; ENSG00000185418. [A2RTX5-1]
GeneIDi123283.
KEGGihsa:123283.
UCSCiuc002bxm.3. human. [A2RTX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056653 mRNA. Translation: BAB71241.1.
AK057734 mRNA. Translation: BAB71554.1. Different initiation.
CH471101 Genomic DNA. Translation: EAX02316.1.
BC069346 mRNA. Translation: AAH69346.1.
BC069811 mRNA. Translation: AAH69811.1.
BC074887 mRNA. Translation: AAH74887.1.
BC132671 mRNA. Translation: AAI32672.1.
BC136315 mRNA. Translation: AAI36316.1.
CCDSiCCDS10394.1. [A2RTX5-1]
RefSeqiNP_689547.2. NM_152334.2. [A2RTX5-1]
UniGeneiHs.657163.

3D structure databases

ProteinModelPortaliA2RTX5.
SMRiA2RTX5. Positions 162-800.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125822. 7 interactions.
STRINGi9606.ENSP00000338093.

PTM databases

PhosphoSiteiA2RTX5.

Proteomic databases

MaxQBiA2RTX5.
PaxDbiA2RTX5.
PRIDEiA2RTX5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335968; ENSP00000338093; ENSG00000185418. [A2RTX5-1]
GeneIDi123283.
KEGGihsa:123283.
UCSCiuc002bxm.3. human. [A2RTX5-1]

Organism-specific databases

CTDi123283.
GeneCardsiGC15M102193.
HGNCiHGNC:24728. TARSL2.
HPAiHPA063963.
neXtProtiNX_A2RTX5.
PharmGKBiPA128394753.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0441.
GeneTreeiENSGT00390000002149.
HOGENOMiHOG000003878.
HOVERGENiHBG059513.
InParanoidiA2RTX5.
KOiK01868.
OMAiKSGHWDK.
OrthoDBiEOG7JDQX3.
PhylomeDBiA2RTX5.
TreeFamiTF300858.

Miscellaneous databases

ChiTaRSiTARSL2. human.
GenomeRNAii123283.
NextBioi81100.
PROiA2RTX5.

Gene expression databases

BgeeiA2RTX5.
CleanExiHS_TARSL2.
ExpressionAtlasiA2RTX5. baseline and differential.
GenevestigatoriA2RTX5.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 315-802 (ISOFORM 1).
    Tissue: Cerebellum.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 337-802 (ISOFORMS 1/2).
    Tissue: Brain and Lung.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYTC2_HUMAN
AccessioniPrimary (citable) accession number: A2RTX5
Secondary accession number(s): B2RMP7
, Q6B0A1, Q6IS76, Q96LW3, Q96MP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 6, 2007
Last modified: March 4, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.