A2RT67 (DEND3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DENN domain-containing protein 3

Gene names

Name:	Dennd3
Synonyms:	Kiaa0870

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1274 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. Thereby, may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Ref.4
Sequence similarities	Contains 1 dDENN domain. Contains 1 DENN domain. Contains 1 uDENN domain. Contains 3 WD repeats.
Sequence caution	The sequence BAC35269.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD32324.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Repeat WD repeat
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein catabolic process Inferred from mutant phenotype Ref.4. Source: UniProtKB endosome to lysosome transport Inferred from genetic interaction Ref.4. Source: UniProtKB
Molecular_function	Rab guanyl-nucleotide exchange factor activity Inferred from genetic interaction Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1274	1274	DENN domain-containing protein 3		PRO_0000304673
Regions
	Domain	84 – 161	78	UDENN
	Domain	187 – 373	187	DENN
	Domain	436 – 499	64	dDENN
	Repeat	1025 – 1063	39	WD 1
	Repeat	1066 – 1107	42	WD 2
	Repeat	1241 – 1274	34	WD 3
Experimental info
	Sequence conflict	126	1	P → S in BAD32324. Ref.1
	Sequence conflict	1003	1	L → Q in BAC35269. Ref.3
	Sequence conflict	1200	1	T → N in BAC35269. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	A2RT67 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: 4C465CE967D72DB5	FASTA	1,274	143,888
	10 20 30 40 50 60 MAEPAARHLS LPSGLLELCA LLGASQDSLR GLEQIAQKRG VKSASSLVPE VLSVFVPPFT 70 80 90 100 110 120 TKEDGQVPGA SCALGKGRRR SFRKKREKPR MEPWKSHPGD SKGPDSEDVT IPGGVDLLAL 130 140 150 160 170 180 PQLCFPGCVC VASEPKEDYI HFLVLTDVCG NRTYGVVAQY YRPLHDEYCF YNGKSHWEPS 190 200 210 220 230 240 VISARCFVPF AVCVVSRFPY YNSLKDCLSC LLTHLKLCKD FEVDNHIKDF AARLSLIPSP 250 260 270 280 290 300 PPGPLHLIFN MKPLQVVFPS RADPESPIVD LDLHLPLLCF RPEKVLQILT CILTEQRIVF 310 320 330 340 350 360 FSSDWALLTL MAECFVAYLH PLQWQHTFVP ILSGQMLDFV MAPTSFLMGC HLDHFEEVRK 370 380 390 400 410 420 EADGLVLIDI DHGSVTCSKS SDDNIDIPDV PLLLAQTFIQ RVQSLQLHPD LHLAHLSAST 430 440 450 460 470 480 DLNEGRARRR AWQQTLNCKI QHITLQLLVG IFREVKNHLN YEHRVFNSEE FLKTRAAGDQ 490 500 510 520 530 540 QFYKQVLDTY MFHSFLKARL NGRMDAFARM DLDTQSEEDR IDRMLISPRR PTVEKMASRK 550 560 570 580 590 600 ASPLHITHRR MVVSMPNLQD ISLPELPPRN SSLRIMDTSN CRSSSPVLKV TPKSTYMFKI 610 620 630 640 650 660 PDIHFPLESQ CVQAYYTDFV TLLSKAMALL GPGDSLLLAR YFYLRGLLHL MQGQLLSALL 670 680 690 700 710 720 DFQNLYKTDI GIFPADLVKR TVESMSASER AQAERTPELR RLITEVFDKH GEAPKADDAV 730 740 750 760 770 780 KNFELPKKHM QLNDFVKRVQ ESGIVKDAVI IHRLFDALTF GHEKQIDPET FRDFYTCWKE 790 800 810 820 830 840 TEAEAQEVSL PALLMEHLDK NECVYKLSSS VKTNRGVGKI AMTQKRLFLL TEGRPGYVEI 850 860 870 880 890 900 ATFRNIEEVK NSTVAFLLLR IPTLKIKTVA KKEVFEANLK SECDLWHLMV KEMWAGKQLA 910 920 930 940 950 960 DDHKDPQYVQ QALTNVLLMD AVVGTLQSPS AIHAASKLAY FDNMKKKSPM AVPKTTSETL 970 980 990 1000 1010 1020 KHKINPSAGE TAPQAIEVLL YTPGRLDPAE KVEDAHPKLW CALNEGKVVV FDASSWTVHQ 1030 1040 1050 1060 1070 1080 HCFKVGSSKV NCMVMAEHNQ VWVGSEDSVI YIINVHSMSC NKQLTDHRSP VTGLAVHNGK 1090 1100 1110 1120 1130 1140 KPSEIYSCSL DGTVIAWNVS TLRVISRFQL SYGDLLSISL HNDRIWCCTV HKILVVTPQG 1150 1160 1170 1180 1190 1200 FVRQELKHPK DASFLAFQLL PEEQQLWAAS TGVSELYMWS LKDLDQPPQK TYLQDCSEVT 1210 1220 1230 1240 1250 1260 CMIRVKRQIW VGGRGLSQGK TRGKIYVMDV EKVTVEKELV AHLDTVRTLC SAEDRYVLSG 1270 AGQEEGKIAI WKVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 495-1274. Strain: C57BL/6J. Tissue: Lung.
[4]	"Small GTPase Rab12 regulates constitutive degradation of transferrin receptor." Matsui T., Itoh T., Fukuda M. Traffic 12:1432-1443(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK173046 mRNA. Translation: BAD32324.1. Different initiation. BC132389 mRNA. Translation: AAI32390.2. BC137791 mRNA. Translation: AAI37792.1. AK053114 mRNA. Translation: BAC35269.1. Different initiation.
IPI	IPI00464312.
RefSeq	NP_001074535.1. NM_001081066.1.
UniGene	Mm.322557.
3D structure databases
ProteinModelPortal	A2RT67.
SMR	A2RT67. Positions 117-500, 998-1136.
ModBase	Search...
PTM databases
PhosphoSite	A2RT67.
Proteomic databases
PaxDb	A2RT67.
PRIDE	A2RT67.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000043414; ENSMUSP00000046774; ENSMUSG00000036661.
GeneID	105841.
KEGG	mmu:105841.
UCSC	uc007wce.1. mouse.
Organism-specific databases
CTD	22898.
MGI	MGI:2146009. Dennd3.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG115977.
GeneTree	ENSGT00700000104558.
HOGENOM	HOG000060114.
HOVERGEN	HBG095574.
InParanoid	B2RQ75.
OMA	LPKKHMQ.
OrthoDB	EOG4P8FH7.
Gene expression databases
ArrayExpress	A2RT67.
Bgee	A2RT67.
CleanEx	MM_DENND3.
Genevestigator	A2RT67.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR005112. dDENN_dom. IPR001194. DENN_dom. IPR005113. uDENN_dom. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF03455. dDENN. 1 hit. PF02141. DENN. 1 hit. PF00400. WD40. 1 hit. [Graphical view]
SMART	SM00801. dDENN. 1 hit. SM00799. DENN. 1 hit. SM00320. WD40. 3 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS50947. DDENN. 1 hit. PS50211. DENN. 1 hit. PS50946. UDENN. 1 hit. PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 1 hit. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	357918.
SOURCE	Search...

Entry information

Entry name

DEND3_MOUSE

Accession

Primary (citable) accession number: A2RT67
Secondary accession number(s): B2RQ75, Q69ZX2, Q8C6V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 20, 2007
Last modified:	May 1, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program