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Protein

tRNA wybutosine-synthesizing protein 5

Gene

Tyw5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes (By similarity).By similarity

Catalytic activityi

7-(3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) + 2-oxoglutarate + O2 = 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) + succinate + CO2.

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1062-oxoglutarateBy similarity1
Metal bindingi160Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi162Iron; catalyticPROSITE-ProRule annotation1
Binding sitei1662-oxoglutarateBy similarity1
Binding sitei1752-oxoglutarateBy similarity1
Metal bindingi235Iron; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 5 (EC:1.14.11.42)
Alternative name(s):
tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase
Gene namesi
Name:Tyw5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915986. Tyw5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003092751 – 315tRNA wybutosine-synthesizing protein 5Add BLAST315

Proteomic databases

EPDiA2RSX7.
MaxQBiA2RSX7.
PaxDbiA2RSX7.
PeptideAtlasiA2RSX7.
PRIDEiA2RSX7.

PTM databases

PhosphoSitePlusiA2RSX7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000048495.
CleanExiMM_1110034B05RIK.
ExpressionAtlasiA2RSX7. baseline and differential.
GenevisibleiA2RSX7. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125427.

Structurei

3D structure databases

ProteinModelPortaliA2RSX7.
SMRiA2RSX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini102 – 267JmjCPROSITE-ProRule annotationAdd BLAST166

Sequence similaritiesi

Belongs to the TYW5 family.Curated
Contains 1 JmjC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2132. Eukaryota.
ENOG410XQDR. LUCA.
GeneTreeiENSGT00530000062914.
HOVERGENiHBG096249.
InParanoidiA2RSX7.
KOiK18066.
OMAiFFREEQF.
OrthoDBiEOG091G0B98.
PhylomeDBiA2RSX7.
TreeFamiTF332364.

Family and domain databases

InterProiIPR003347. JmjC_dom.
IPR029612. TYW5.
[Graphical view]
PANTHERiPTHR12461:SF50. PTHR12461:SF50. 1 hit.
SMARTiSM00558. JmjC. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2RSX7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEQRLPVPR LRGVSREQFM EHLYPQRKPL VLEGLDLGSC TSKWTVDYLS
60 70 80 90 100
QVGGTKEVKI HVAAVPQMDF ISKNFVYRTL PFNKLVQRAA EETHKEFFIS
110 120 130 140 150
EDEKYYLRSL GEDPRKDVAD IRQQFPSLGG DITFPMFFRE EQFFSSVFRI
160 170 180 190 200
SSPGLQLWTH YDVMDNFLIQ VTGKKRITLF NPRDAQYLYL SGSKSEVLNI
210 220 230 240 250
DSPDLDKYPL FPKARRYECS LEAGDVLFIP ALWFHNVVSE EFGVGVNIFW
260 270 280 290 300
KHLPSECYDT TDTYGNKDPV AASRAVQILD RALKTLAELP EEYRDFYARQ
310
MVLRIQDKAY SKNFE
Length:315
Mass (Da):36,633
Last modified:November 13, 2007 - v2
Checksum:i26B51EF4662C55A3
GO
Isoform 2 (identifier: A2RSX7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-121: SKNFVYRTLP...EDPRKDVADI → KLYLLTSWSR...LQTSDSSSHH
     122-315: Missing.

Show »
Length:121
Mass (Da):14,024
Checksum:i313F14E41B27AA96
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5R → H in AAI32290 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02910772 – 121SKNFV…DVADI → KLYLLTSWSREQPKKHIKNS SFQRMRNTTYGHLEKTQGRM LQTSDSSSHH in isoform 2. 2 PublicationsAdd BLAST50
Alternative sequenceiVSP_029108122 – 315Missing in isoform 2. 2 PublicationsAdd BLAST194

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139666 mRNA. Translation: BAE24098.1.
BC099495 mRNA. No translation available.
BC132289 mRNA. Translation: AAI32290.1.
CCDSiCCDS14966.2. [A2RSX7-1]
RefSeqiNP_001032831.2. NM_001037742.2. [A2RSX7-1]
UniGeneiMm.288490.

Genome annotation databases

EnsembliENSMUST00000079998; ENSMUSP00000078912; ENSMUSG00000048495. [A2RSX7-2]
ENSMUST00000162686; ENSMUSP00000125427; ENSMUSG00000048495. [A2RSX7-1]
GeneIDi68736.
KEGGimmu:68736.
UCSCiuc007baz.2. mouse. [A2RSX7-1]
uc007bbb.2. mouse. [A2RSX7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139666 mRNA. Translation: BAE24098.1.
BC099495 mRNA. No translation available.
BC132289 mRNA. Translation: AAI32290.1.
CCDSiCCDS14966.2. [A2RSX7-1]
RefSeqiNP_001032831.2. NM_001037742.2. [A2RSX7-1]
UniGeneiMm.288490.

3D structure databases

ProteinModelPortaliA2RSX7.
SMRiA2RSX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125427.

PTM databases

PhosphoSitePlusiA2RSX7.

Proteomic databases

EPDiA2RSX7.
MaxQBiA2RSX7.
PaxDbiA2RSX7.
PeptideAtlasiA2RSX7.
PRIDEiA2RSX7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079998; ENSMUSP00000078912; ENSMUSG00000048495. [A2RSX7-2]
ENSMUST00000162686; ENSMUSP00000125427; ENSMUSG00000048495. [A2RSX7-1]
GeneIDi68736.
KEGGimmu:68736.
UCSCiuc007baz.2. mouse. [A2RSX7-1]
uc007bbb.2. mouse. [A2RSX7-2]

Organism-specific databases

CTDi129450.
MGIiMGI:1915986. Tyw5.

Phylogenomic databases

eggNOGiKOG2132. Eukaryota.
ENOG410XQDR. LUCA.
GeneTreeiENSGT00530000062914.
HOVERGENiHBG096249.
InParanoidiA2RSX7.
KOiK18066.
OMAiFFREEQF.
OrthoDBiEOG091G0B98.
PhylomeDBiA2RSX7.
TreeFamiTF332364.

Enzyme and pathway databases

UniPathwayiUPA00375.

Miscellaneous databases

PROiA2RSX7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048495.
CleanExiMM_1110034B05RIK.
ExpressionAtlasiA2RSX7. baseline and differential.
GenevisibleiA2RSX7. MM.

Family and domain databases

InterProiIPR003347. JmjC_dom.
IPR029612. TYW5.
[Graphical view]
PANTHERiPTHR12461:SF50. PTHR12461:SF50. 1 hit.
SMARTiSM00558. JmjC. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYW5_MOUSE
AccessioniPrimary (citable) accession number: A2RSX7
Secondary accession number(s): Q3UT82, Q4KL17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: November 2, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.