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Protein

Glycogen [starch] synthase, muscle

Gene

Gys1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.By similarity

Catalytic activityi

UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

Enzyme regulationi

Allosteric activation by glucose-6-phosphate. Phosphorylation reduces the activity towards UDP-glucose. When in the non-phosphorylated state, glycogen synthase does not require glucose-6-phosphate as an allosteric activator; when phosphorylated it does (By similarity).By similarity

Pathway: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391UDP-glucoseBy similarity

GO - Molecular functioni

GO - Biological processi

  • glycogen biosynthetic process Source: RGD
  • glycogen metabolic process Source: RGD
  • heart development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

ReactomeiREACT_277069. Glycogen synthesis.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen [starch] synthase, muscle (EC:2.4.1.11)
Gene namesi
Name:Gys1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1589798. Gys1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 738737Glycogen [starch] synthase, musclePRO_0000366920Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81Phosphoserine; by AMPK and PKABy similarity
Modified residuei11 – 111PhosphoserineBy similarity
Modified residuei412 – 4121PhosphoserineBy similarity
Modified residuei641 – 6411Phosphoserine; by DYRK2, GSK3-alpha, GSK3-beta and PASKBy similarity
Modified residuei645 – 6451Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity
Modified residuei649 – 6491Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity
Modified residuei653 – 6531Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity
Modified residuei657 – 6571Phosphoserine; by CK2By similarity
Modified residuei698 – 6981PhosphoserineBy similarity
Modified residuei728 – 7281PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Phosphorylated at Ser-641 by DYRK2, leading to inactivation. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA2RRU1.
PRIDEiA2RRU1.

PTM databases

PhosphoSiteiA2RRU1.

Expressioni

Gene expression databases

GenevisibleiA2RRU1. RN.

Interactioni

Subunit structurei

Interacts with GYG1.By similarity

Protein-protein interaction databases

BioGridi605796. 1 interaction.
STRINGi10116.ENSRNOP00000028271.

Structurei

3D structure databases

ProteinModelPortaliA2RRU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 3 family.Curated

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
HOVERGENiHBG001960.
InParanoidiA2RRU1.
KOiK00693.
OMAiFAMKRHG.
OrthoDBiEOG741Z1N.
PhylomeDBiA2RRU1.
TreeFamiTF300306.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2RRU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLSRSLSMS SLPGLEDWED EFDPENAVLF EVAWEVANKV GGIYTVLQTK
60 70 80 90 100
AKVTGDEWGD NYYLVGPYTE QGVRTQVELL EPPTPELKRT LDSMNSKGCK
110 120 130 140 150
VYFGRWLIEG GPLVVLLDVG ASAWALERWK GELWDTCNIG VPWYDREAND
160 170 180 190 200
AVLFGFLTTW FLGEFLAQNE EKPYVVAHFH EWLAGVGLCL CRARRLPVAT
210 220 230 240 250
IFTTHATLLG RYLCAGAVDF YNNLENFNVD KEAGERQIYH RYCMERAAAH
260 270 280 290 300
CAHVFTTVSQ ITAIEAQHLL KRKPDIVTPN GLNVKKFSAM HEFQNLHAQS
310 320 330 340 350
KARIQEFVRG HFYGHLDFNL DKTLYFFIAG RYEFSNKGAD IFLEALARLN
360 370 380 390 400
YLLRVNGSEQ TVVAFFIMPA RTNNFNVETL KGQAVRKQLW DTANTVKEKF
410 420 430 440 450
GRKLYESLLV GSLPDMNKML DKEDFTMMKR AIFATQRQSF PPVCTHNMLD
460 470 480 490 500
DSSDPILTTI RRIGLFNSSA DRVKVIFHPE FLSSTSPLLP VDYEEFVRGC
510 520 530 540 550
HLGVFPSYYE PWGYTPAECT VMGIPSISTN LSGFGCFMEE HIADPSAYGI
560 570 580 590 600
YILDRRFRSL DDSCSQLTSF LYSFCQQSRR QRIIQRNRTE RLSDLLDWKY
610 620 630 640 650
LGRYYMSARH MALAKAFPDH FTYEPHEVDA TQGYRYPRPA SVPPSPSLSR
660 670 680 690 700
HSSPHQSEDE EEPRDGPLRE DSERYDEEEE AAKDRRNIRA PEWPRRASCS
710 720 730
SSTGGSKRSN SVDTGPSSSL STPTEPLSPT SSLGEERN
Length:738
Mass (Da):84,072
Last modified:March 6, 2007 - v1
Checksum:i912823E398F5263C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC131849 mRNA. Translation: AAI31850.1.
RefSeqiNP_001103085.1. NM_001109615.1.
UniGeneiRn.95278.

Genome annotation databases

EnsembliENSRNOT00000028271; ENSRNOP00000028271; ENSRNOG00000020812.
GeneIDi690987.
KEGGirno:690987.
UCSCiRGD:1589798. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC131849 mRNA. Translation: AAI31850.1.
RefSeqiNP_001103085.1. NM_001109615.1.
UniGeneiRn.95278.

3D structure databases

ProteinModelPortaliA2RRU1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi605796. 1 interaction.
STRINGi10116.ENSRNOP00000028271.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

PTM databases

PhosphoSiteiA2RRU1.

Proteomic databases

PaxDbiA2RRU1.
PRIDEiA2RRU1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028271; ENSRNOP00000028271; ENSRNOG00000020812.
GeneIDi690987.
KEGGirno:690987.
UCSCiRGD:1589798. rat.

Organism-specific databases

CTDi2997.
RGDi1589798. Gys1.

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
HOVERGENiHBG001960.
InParanoidiA2RRU1.
KOiK00693.
OMAiFAMKRHG.
OrthoDBiEOG741Z1N.
PhylomeDBiA2RRU1.
TreeFamiTF300306.

Enzyme and pathway databases

UniPathwayiUPA00164.
ReactomeiREACT_277069. Glycogen synthesis.

Miscellaneous databases

NextBioi742114.
PROiA2RRU1.

Gene expression databases

GenevisibleiA2RRU1. RN.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiGYS1_RAT
AccessioniPrimary (citable) accession number: A2RRU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 6, 2007
Last modified: June 24, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.