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Protein

Cytochrome P450 4V2

Gene

Cyp4v2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Omega-hydroxylase that oxidizes medium-chain saturated fatty acids and polyunsaturated omega-3 fatty acids, and which plays a role in fatty acid and steroid metabolism in the eye. Catalyzes the omega-hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. The substrate specificity is higher for myristate > laurate > palmitate (C14>C16>C12). Acts as a polyunsaturated omega-3 fatty acids hydroxylase by mediating oxidation of docosahexaenoate (DHA) to 22-hydroxydocosahexaenoate. Also produces some 21-hydroxydocosahexaenoate. Also converts eicosapentaenoate (EPA) to 20-hydroxyeicosapentaenoate (20-OH-EPA).By similarity

Catalytic activityi

Docosahexaenoate + NADPH + O2 = 22-hydroxydocosahexaenoate + NADP+ + H2O.By similarity

Cofactori

hemeBy similarity

Enzyme regulationi

Inhibited by N-hydroxy-N'-(4-n-butyl-2-methylphenyl formamidine)(HET0016) with an IC50 of 38 nM.By similarity

Pathwayi: Lipid metabolism

This protein is involved in Lipid metabolism.Curated
View all proteins of this organism that are known to be involved in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei329 – 3291Heme (covalent; via 1 link)By similarity
Metal bindingi467 – 4671Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-RNO-211976. Endogenous sterols.
R-RNO-2453902. The canonical retinoid cycle in rods (twilight vision).

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4V2 (EC:1.14.13.-)
Alternative name(s):
Docosahexaenoic acid omega-hydroxylase CYP4V2Curated (EC:1.14.13.199By similarity)
Gene namesi
Name:Cyp4v2
Synonyms:Cyp4v3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi708530. Cyp4v3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Cytochrome P450 4V2PRO_0000311708Add
BLAST

Proteomic databases

PaxDbiA2RRT9.
PRIDEiA2RRT9.

Expressioni

Gene expression databases

GenevisibleiA2RRT9. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019302.

Structurei

3D structure databases

ProteinModelPortaliA2RRT9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOVERGENiHBG000182.
InParanoidiA2RRT9.
KOiK07427.
OMAiREFWIES.
OrthoDBiEOG7CNZFK.
PhylomeDBiA2RRT9.
TreeFamiTF105088.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2RRT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWLWLGLSG QKLLLWGAAS AVSVAGATVL LNILQMLVSY ARKWQQMRPI
60 70 80 90 100
PSVARAYPLV GHALFMKPNN TEFFQQIIQY TEEFRHLPII KLWIGPVPLV
110 120 130 140 150
ALYKAENVEV ILTSSKQIDK SFMYKFLQPW LGLGLLTSTG SKWRARRKML
160 170 180 190 200
TPSFHFTILE DFLDVMNEQA NILVNKLEKH VNQEAFNCFF PITLCALDII
210 220 230 240 250
CETAMGKNIG AQSNGDSEYV RTVYRMSDMI YRRMKMPWFW FDLWYLMFKE
260 270 280 290 300
GRDHKKGLKS LHTFTNNVIA ERVNARKAEQ DCIGAGRGPL PSKTKRKAFL
310 320 330 340 350
DLLLSVTDEE GNKLSHEDIR EEVDTFMFEG HDTTAAAINW SLYLLGSNPE
360 370 380 390 400
VQRKVDKELD DVFGRSHRPV TLEDLKKLKY LDCVIKETLR VFPSVPLFAR
410 420 430 440 450
SLSEDCEVAG YKISKGTEAV IIPYALHRDP RYFPDPEEFQ PERFFPENSQ
460 470 480 490 500
GRHPYAYVPF SAGPRNCIGQ KFAVMEEKTI LACILREFWI ESNQKREELG
510 520
LAGDLILRPN NGIWIKLKRR HEDDP
Length:525
Mass (Da):60,580
Last modified:March 6, 2007 - v1
Checksum:iD93E3911A6B5D5BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC131846 mRNA. Translation: AAI31847.1.
RefSeqiNP_001129072.1. NM_001135600.1.
UniGeneiRn.201722.

Genome annotation databases

EnsembliENSRNOT00000019302; ENSRNOP00000019302; ENSRNOG00000042426.
GeneIDi266761.
KEGGirno:266761.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC131846 mRNA. Translation: AAI31847.1.
RefSeqiNP_001129072.1. NM_001135600.1.
UniGeneiRn.201722.

3D structure databases

ProteinModelPortaliA2RRT9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019302.

Proteomic databases

PaxDbiA2RRT9.
PRIDEiA2RRT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019302; ENSRNOP00000019302; ENSRNOG00000042426.
GeneIDi266761.
KEGGirno:266761.

Organism-specific databases

CTDi102294.
RGDi708530. Cyp4v3.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOVERGENiHBG000182.
InParanoidiA2RRT9.
KOiK07427.
OMAiREFWIES.
OrthoDBiEOG7CNZFK.
PhylomeDBiA2RRT9.
TreeFamiTF105088.

Enzyme and pathway databases

ReactomeiR-RNO-211976. Endogenous sterols.
R-RNO-2453902. The canonical retinoid cycle in rods (twilight vision).

Miscellaneous databases

PROiA2RRT9.

Gene expression databases

GenevisibleiA2RRT9. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: LungImported.

Entry informationi

Entry nameiCP4V2_RAT
AccessioniPrimary (citable) accession number: A2RRT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 6, 2007
Last modified: June 8, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.