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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22UDP-GlcNAcUniRule annotation1
Binding sitei72UDP-GlcNAcUniRule annotation1
Metal bindingi102MagnesiumUniRule annotation1
Binding sitei139UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei154UDP-GlcNAcUniRule annotation1
Binding sitei169UDP-GlcNAcUniRule annotation1
Metal bindingi227MagnesiumUniRule annotation1
Binding sitei227UDP-GlcNAcUniRule annotation1
Binding sitei332UDP-GlcNAcUniRule annotation1
Binding sitei350UDP-GlcNAcUniRule annotation1
Active sitei362Proton acceptorUniRule annotation1
Binding sitei365UDP-GlcNAcUniRule annotation1
Binding sitei376UDP-GlcNAcUniRule annotation1
Binding sitei379Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei404Acetyl-CoAUniRule annotation1
Binding sitei422Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei439Acetyl-CoAUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00113; UER00532
UPA00113; UER00533
UPA00973

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:llmg_2076
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000000364 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000561691 – 458Bifunctional protein GlmUAdd BLAST458

Proteomic databases

PRIDEiA2RMV7

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi416870.llmg_2076

Structurei

3D structure databases

ProteinModelPortaliA2RMV7
SMRiA2RMV7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 229PyrophosphorylaseUniRule annotationAdd BLAST229
Regioni8 – 11UDP-GlcNAc bindingUniRule annotation4
Regioni77 – 78UDP-GlcNAc bindingUniRule annotation2
Regioni230 – 250LinkerUniRule annotationAdd BLAST21
Regioni251 – 458N-acetyltransferaseUniRule annotationAdd BLAST208

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CAJ Bacteria
COG1207 LUCA
HOGENOMiHOG000283476
KOiK04042
OMAiIEPQTHL

Family and domain databases

CDDicd03353 LbH_GlmU_C, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_01631 GlmU, 1 hit
InterProiView protein in InterPro
IPR005882 Bifunctional_GlmU
IPR038009 GlmU_C_LbH
IPR001451 Hexapep
IPR025877 MobA-like_NTP_Trfase
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
PfamiView protein in Pfam
PF00132 Hexapep, 1 hit
PF12804 NTP_transf_3, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR01173 glmU, 1 hit

Sequencei

Sequence statusi: Complete.

A2RMV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKFAIVLAA GKGTRMKSAL PKVLHQVAGK SMLAHVLKSV SEVEIAKNVV
60 70 80 90 100
IVGHEADRVI ATLPKGTQFV KQVEQLGTGH AVRIAADLLA NEEGATLVIA
110 120 130 140 150
GDTPLITGET LGALFDYHFA QKATATILTA IAPNPTGYGR IIRDEKDSVE
160 170 180 190 200
KIVEQKDANA FEKSITEINT GTYIFDNKSL FKALTEITTD NAQGEYYLTD
210 220 230 240 250
VIEIFKKVGQ TIAAHILDDF DESLGVNDRV ALSQAEATMR KRINHEHMVN
260 270 280 290 300
GVTLIDPATT YIDSEVTIGA ETVIEANVTI KGNTFIGKNV LITNGSRIEN
310 320 330 340 350
SEIHSNCEVR NSTVEESRMS VGSNVGPYAH LRPGTVLSEE VHVGNFVEIK
360 370 380 390 400
GSTLGKGTKA GHLTYIGNAT VGEKVNFGAG TITANFDGKN KFNTEIDDFA
410 420 430 440 450
FIGSNSTIIA PLHIGKNALT AAGSVVTEDV PDEAVEIGRG KQVNKLGRAK

KMPHYRGQ
Length:458
Mass (Da):49,122
Last modified:March 6, 2007 - v1
Checksum:i316B0BCDDC8F6546
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406671 Genomic DNA Translation: CAL98643.1
RefSeqiWP_011835789.1, NC_009004.1

Genome annotation databases

EnsemblBacteriaiCAL98643; CAL98643; llmg_2076
KEGGillm:llmg_2076

Similar proteinsi

Entry informationi

Entry nameiGLMU_LACLM
AccessioniPrimary (citable) accession number: A2RMV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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