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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi490MagnesiumUniRule annotation1
Metal bindingi496MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:llmg_2044
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000000364 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003296881 – 766Polyribonucleotide nucleotidyltransferaseAdd BLAST766

Interactioni

Protein-protein interaction databases

STRINGi416870.llmg_2044.

Structurei

3D structure databases

ProteinModelPortaliA2RMS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini557 – 616KHUniRule annotationAdd BLAST60
Domaini626 – 694S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2RMS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKETFSIEF AGRTLTVETG QVAKQANGAV VVRYGDTTVL TAATMGKMAT
60 70 80 90 100
GDFFPLQVNY EEKMYAAGKF PGGFNKREAR PSTDATLTAR LIDRPIRPMF
110 120 130 140 150
AEGFRNEVQV INTVLSYDEN ASGRVAAMFG SSLALAISDI PFDGPIAGVE
160 170 180 190 200
VAYIDGKYII NPTVAEKEAS SLELSVAGNI NAINMVESGA KELSEEVMLG
210 220 230 240 250
ALLAGHNAVK ELIEFQNEIV AKVGKEKAEV ELLHVDEDLK AEVIAAYNSD
260 270 280 290 300
LQKAVQVEEK LAREAATKAV KEAIISVYSA KYENDENLSI ILRDLAEILE
310 320 330 340 350
GMEHAEVRRL ITEDKIRPDG RKIDEIRPLD AEIDFTPRSI THGTGLFTRG
360 370 380 390 400
QTQALSTLTL APMNEAQIID GLNDEYKKRF MHHYNFPQYS VGETGRYGAP
410 420 430 440 450
GRREIGHGAL GERALEQVLP SLEEFPYAIR LVAEVLESNG SSSQASICAG
460 470 480 490 500
TLALMAGGVP IKAPVAGIAM GLISDGTNYT VLTDIQGLED HFGDMDFKVA
510 520 530 540 550
GTREGITALQ MDIKISGITP EILAEALAQA KTARFQILDV IEATIAQPRE
560 570 580 590 600
ELAPSAPKID TITIPVDKIK VVIGKGGEQI DKIIAETGVK IDIDDEGLCS
610 620 630 640 650
IFSSDQSAID RAKEIIAELV REAKVGEVYE AKVVRIESFG AFVNLFGKQD
660 670 680 690 700
AMVHISEMAW ARTAKVEDVM KLGDVVKVKI MKIDDKGRVD ASMRALIEKP
710 720 730 740 750
EGYVEPERKP RERRDNKDRR NGNGFDRRNN DRNNHNNNSG NHSFELRERK
760
SHVDHEFPEL STKKPE
Length:766
Mass (Da):83,920
Last modified:March 6, 2007 - v1
Checksum:i4DD49938BFF46DD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406671 Genomic DNA. Translation: CAL98611.1.
RefSeqiWP_011835761.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL98611; CAL98611; llmg_2044.
KEGGillm:llmg_2044.
PATRICi22285280. VBILacLac4574_2092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406671 Genomic DNA. Translation: CAL98611.1.
RefSeqiWP_011835761.1. NC_009004.1.

3D structure databases

ProteinModelPortaliA2RMS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416870.llmg_2044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL98611; CAL98611; llmg_2044.
KEGGillm:llmg_2044.
PATRICi22285280. VBILacLac4574_2092.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_LACLM
AccessioniPrimary (citable) accession number: A2RMS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 6, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.