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A2RMS5

- PNP_LACLM

UniProt

A2RMS5 - PNP_LACLM

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, llmg_2044
Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi490 – 4901Magnesium By similarity
Metal bindingi496 – 4961Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-2046-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:llmg_2044
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000000364: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 766766Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329688Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi416870.llmg_2044.

Structurei

3D structure databases

ProteinModelPortaliA2RMS5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini557 – 61660KH
Add
BLAST
Domaini626 – 69469S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2RMS5-1 [UniParc]FASTAAdd to Basket

« Hide

MAKETFSIEF AGRTLTVETG QVAKQANGAV VVRYGDTTVL TAATMGKMAT    50
GDFFPLQVNY EEKMYAAGKF PGGFNKREAR PSTDATLTAR LIDRPIRPMF 100
AEGFRNEVQV INTVLSYDEN ASGRVAAMFG SSLALAISDI PFDGPIAGVE 150
VAYIDGKYII NPTVAEKEAS SLELSVAGNI NAINMVESGA KELSEEVMLG 200
ALLAGHNAVK ELIEFQNEIV AKVGKEKAEV ELLHVDEDLK AEVIAAYNSD 250
LQKAVQVEEK LAREAATKAV KEAIISVYSA KYENDENLSI ILRDLAEILE 300
GMEHAEVRRL ITEDKIRPDG RKIDEIRPLD AEIDFTPRSI THGTGLFTRG 350
QTQALSTLTL APMNEAQIID GLNDEYKKRF MHHYNFPQYS VGETGRYGAP 400
GRREIGHGAL GERALEQVLP SLEEFPYAIR LVAEVLESNG SSSQASICAG 450
TLALMAGGVP IKAPVAGIAM GLISDGTNYT VLTDIQGLED HFGDMDFKVA 500
GTREGITALQ MDIKISGITP EILAEALAQA KTARFQILDV IEATIAQPRE 550
ELAPSAPKID TITIPVDKIK VVIGKGGEQI DKIIAETGVK IDIDDEGLCS 600
IFSSDQSAID RAKEIIAELV REAKVGEVYE AKVVRIESFG AFVNLFGKQD 650
AMVHISEMAW ARTAKVEDVM KLGDVVKVKI MKIDDKGRVD ASMRALIEKP 700
EGYVEPERKP RERRDNKDRR NGNGFDRRNN DRNNHNNNSG NHSFELRERK 750
SHVDHEFPEL STKKPE 766
Length:766
Mass (Da):83,920
Last modified:March 6, 2007 - v1
Checksum:i4DD49938BFF46DD0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM406671 Genomic DNA. Translation: CAL98611.1.
RefSeqiYP_001033299.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL98611; CAL98611; llmg_2044.
GeneIDi4799121.
KEGGillm:llmg_2044.
PATRICi22285280. VBILacLac4574_2092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM406671 Genomic DNA. Translation: CAL98611.1 .
RefSeqi YP_001033299.1. NC_009004.1.

3D structure databases

ProteinModelPortali A2RMS5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416870.llmg_2044.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL98611 ; CAL98611 ; llmg_2044 .
GeneIDi 4799121.
KEGGi llm:llmg_2044.
PATRICi 22285280. VBILacLac4574_2092.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci LLAC416870:GCDT-2046-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
    Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
    J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MG1363.

Entry informationi

Entry nameiPNP_LACLM
AccessioniPrimary (citable) accession number: A2RMS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 6, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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