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A2RLY6 (SYL_LACLM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:llmg_1741
OrganismLactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]
Taxonomic identifier416870 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 829829Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009359

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif609 – 6135"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6121ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A2RLY6 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 1502EA4E0E0BF67F

FASTA82993,790
        10         20         30         40         50         60 
MEYNHQKIEA KWQKYWADNK TFRTGTDKNK PKFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRYKRAQGF NVLHPMGWDA FGLPAEQYAM DTGNDPAEFT AENIANFKRQ INSLGFSYDW 

       130        140        150        160        170        180 
EREVNTTDPN FYKWTQWIFT KLYEKGLAYE AEVAVNWVEE LGTAIANEEV LPDGTSERGG 

       190        200        210        220        230        240 
YPVVRKPMRQ WMLKITAYAE RLLEDLEEVD WPESIKEMQR NWIGKSVGAD VTFEVAGTDK 

       250        260        270        280        290        300 
SFEVFTTRPD TLFGATYAVL APEHDLVDAI TTPEQKEAVA EYRRKASLKS DLARTDLSKE 

       310        320        330        340        350        360 
KTGAFTGAYA INPINGRKMP IWVADYVLAS YGHGAVMAVP AHDERDWEFA KVYGLEILPV 

       370        380        390        400        410        420 
VEGGNVEEAV YTEDGPHINS EFLNGLDKAQ AIEKAIEFLE EKKIGKKKIT YRLRDWLFSR 

       430        440        450        460        470        480 
QRYWGEPIPI IHWEDGTSTA LSEDELPLVL PVTSDIKPSG TGESPLANLT DWLEVTRADG 

       490        500        510        520        530        540 
LKGRRETNTM PQWAGSSWYY LRYIDPNNSE ALADPELLKE WLPVDIYVGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLYD LGVVPTKEPF QKLFNQGMIL GTSYRDHRGA LVATDKVEKR DGGFYHMETG 

       610        620        630        640        650        660 
EALEQAPAKM SKSLKNVVNP DDVVEHYGAD TLRVYEMFMG PLDASIPWSE EGLEGARKFL 

       670        680        690        700        710        720 
DRAVRMIENS EIKAENNGEL DKVYNETVKN VTERLDLMYF NTAISQLMIF VNAVNKAKAL 

       730        740        750        760        770        780 
PLEYANGFVQ LLAPFAPHIA EELWVKLGNE AGISYVAWPT FDESKLIESE VEIVVQINGK 

       790        800        810        820 
LKAKIKIAKD LAREELEKIG RESVAEALEG KNVVKVIAVP NKLVNIVVK 

« Hide

References

[1]"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MG1363.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM406671 Genomic DNA. Translation: CAL98313.1.
RefSeqYP_001033014.1. NC_009004.1.

3D structure databases

ProteinModelPortalA2RLY6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416870.llmg_1741.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL98313; CAL98313; llmg_1741.
GeneID4797994.
KEGGllm:llmg_1741.
PATRIC22284667. VBILacLac4574_1788.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycLLAC416870:GCDT-1747-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACLM
AccessionPrimary (citable) accession number: A2RLY6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries