Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-1603-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:llmg_1595
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000000364 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Formate--tetrahydrofolate ligasePRO_0000300525Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi416870.llmg_1595.

Structurei

3D structure databases

ProteinModelPortaliA2RLK1.
SMRiA2RLK1. Positions 4-553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2RLK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDIEIAQA AEIQPITKIA EKIGLSFDDI ELYGKYKAKI PLEVLEKFDE
60 70 80 90 100
QNDGKLVLVT SINPTPAGEG KSTVTVGLAD AFARQDKNVM VALREPSLGP
110 120 130 140 150
VMGIKGGAAG GGFAQVLPME DINLHFTGDI HAITTANNAI SAFLDNSLHQ
160 170 180 190 200
GNPLNIDPRR IIWKRVLDLN DRALRHVTIG LGGPLNGVPR EDGFDITVAS
210 220 230 240 250
EIMAVLCLAT SISDLKERLA RIVIAQNYDR KPVSVGDLGV QGAIAMLLKD
260 270 280 290 300
ALKPNLVQTI EGTPALIHGG PFANIAHGCN SVLATKTALK LADIVITEAG
310 320 330 340 350
FGADLGGEKF LDIKTRQLGK QPDAVVIVAT LRALKMHGGV DKKELTSENV
360 370 380 390 400
EAVKKGFANL ERHIKNMQSY GLPVIVAINQ FASDTESEIS TLKELTEALG
410 420 430 440 450
VSVSLTQVFA KGGEGGLDLA EKLSAMLQAK PDFRYLYELN QPLSVKLDKV
460 470 480 490 500
VTEIYGGSKV NLSPKAKRQM REIEENGWNN LPVCMAKTQY SFSDQANLLA
510 520 530 540 550
APEGFEVTVR ELIPKIGAGF IVALLGDVMT MPGLPKNPAA LKMDVTDDGK

ISGLF
Length:555
Mass (Da):59,516
Last modified:March 6, 2007 - v1
Checksum:iE137C8573039AE67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406671 Genomic DNA. Translation: CAL98169.1.
RefSeqiYP_001032879.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL98169; CAL98169; llmg_1595.
KEGGillm:llmg_1595.
PATRICi22284360. VBILacLac4574_1635.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406671 Genomic DNA. Translation: CAL98169.1.
RefSeqiYP_001032879.1. NC_009004.1.

3D structure databases

ProteinModelPortaliA2RLK1.
SMRiA2RLK1. Positions 4-553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416870.llmg_1595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL98169; CAL98169; llmg_1595.
KEGGillm:llmg_1595.
PATRICi22284360. VBILacLac4574_1635.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciLLAC416870:GCDT-1603-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
    Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
    J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MG1363.

Entry informationi

Entry nameiFTHS_LACLM
AccessioniPrimary (citable) accession number: A2RLK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 6, 2007
Last modified: April 29, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.