Reviewed, UniProtKB/Swiss-Prot A2RJT9 (PYRDA_LACLM)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase A
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase A
    DHOdehase A
      Short name=DHODase A
      Short name=DHOD A

Gene names

Name:	pyrDA
Ordered Locus Names:	llmg_0952

Organism

Lactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]

Taxonomic identifier

416870 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00224
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00224
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (O2 route): step 1/1. HAMAP MF_00224
Subunit structure	Homodimer By similarity. HAMAP MF_00224
Subcellular location	Cytoplasm By similarity HAMAP MF_00224.
Miscellaneous	Can use fumarate but not NAD as an electron acceptor. HAMAP MF_00224
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Dihydroorotate dehydrogenase A HAMAP MF_00224		PRO_0000285239
Sites
	Active site	130	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2RJT9-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 30157E3C2791CDD7	FASTA	311	34,210
	10 20 30 40 50 60 MLNTTFANAK FANPFMNASG VHCMTIEDLE ELKASQAGAY ITKSSTLEKR EGNPLPRYVD 70 80 90 100 110 120 LELGSINSMG LPNLGFDYYL DYVLKNQKEN AQEGPIFFSI AGMSAAENIA MLKKIQESDF 130 140 150 160 170 180 SGITELNLSC PNVPGKPQLA YDFEATEKLL KEVFTFFTKP LGVKLPPYFD LVHFDIMAEI 190 200 210 220 230 240 LNQFPLTYVN SVNSIGNGLF IDPEAESVVI KPKDGFGGIG GAYIKPTALA NVRAFYTRLK 250 260 270 280 290 300 PEIQIIGTGG IETGQDAFEH LLCGATMLQI GTALHKEGPA IFDRIIKELE EIMNQKGYQS 310 IADFHGKLKS L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two different dihydroorotate dehydrogenases in Lactococcus lactis." Andersen P.S., Jansen P.J.G., Hammer K. J. Bacteriol. 176:3975-3982(1994) [PubMed: 8021180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363." Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J. J. Bacteriol. 189:3256-3270(2007) [PubMed: 17307855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74206 Genomic DNA. Translation: CAA52279.1. AM406671 Genomic DNA. Translation: CAL97544.1.
RefSeq	YP_001032274.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2RJT9.
Genome annotation databases
GeneID	4798428.
GenomeReviews	Gene locus llmg_0952 in contig AM406671_GR.
KEGG	llm:llmg_0952.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0167.
HOGENOM	HBG472415.
OMA	KPLGVKL.
Family and domain databases
HAMAP	MF_00224_B. DHO_dh_type1_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005720. Dihydroorotate_DH_1_core. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRDA_LACLM

Accession

Primary (citable) accession number: A2RJT9
Secondary accession number(s): P54321

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 6, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)