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A2RI94 (NADK_LACLM) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:llmg_0383
OrganismLactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]
Taxonomic identifier416870 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270NAD kinase HAMAP-Rule MF_00361
PRO_1000005418

Regions

Nucleotide binding49 – 502NAD By similarity
Nucleotide binding126 – 1272NAD By similarity
Nucleotide binding165 – 1706NAD By similarity

Sites

Active site491Proton acceptor By similarity
Binding site541NAD By similarity
Binding site1521NAD By similarity
Binding site1541NAD By similarity
Binding site1891NAD; via carbonyl oxygen By similarity
Binding site2271NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A2RI94 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 5F8D22CD34712DAD

FASTA27030,343
        10         20         30         40         50         60 
MNFGKKVWLI GNSSEKSKKT LNKLSKILKA EHFVFDDINP EIVISVGGDG TLLRAMHMYE 

        70         80         90        100        110        120 
YQLDRVRFLG VHTGHLGFYT DFTDEDLFEV VEALYDENPA QAIHYPLICV QVSFTDGYQI 

       130        140        150        160        170        180 
VRHVLNEATI RRASKTMVGD VRISDYLFER FRGDGLSIST PTGSTAYNKS IGGAVVHPRV 

       190        200        210        220        230        240 
KAMQIAEIAS LNNVVYRTLG SPMIVAEKDT ITVCPAPEDD YSLTFDQLTF EYKNIKSIEF 

       250        260        270 
SLDGTTISFA NCAHTPFWER VSKSFIGEVE 

« Hide

References

[1]"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MG1363.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM406671 Genomic DNA. Translation: CAL96988.1.
RefSeqYP_001031735.1. NC_009004.1.

3D structure databases

ProteinModelPortalA2RI94.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416870.llmg_0383.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL96988; CAL96988; llmg_0383.
GeneID4797928.
KEGGllm:llmg_0383.
PATRIC22281812. VBILacLac4574_0394.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000275803.
KOK00858.
OMAQWITIVP.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycLLAC416870:GCDT-395-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_LACLM
AccessionPrimary (citable) accession number: A2RI94
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: July 9, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families