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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi289Magnesium or manganese 1UniRule annotation1
Metal bindingi302Magnesium or manganese 1UniRule annotation1
Metal bindingi302Magnesium or manganese 2UniRule annotation1
Metal bindingi304Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 217ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC416870:G1G1R-375-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:llmg_0360
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000000364 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000304551 – 349D-alanine--D-alanine ligaseAdd BLAST349

Proteomic databases

PRIDEiA2RI72

Interactioni

Protein-protein interaction databases

STRINGi416870.llmg_0360

Structurei

3D structure databases

ProteinModelPortaliA2RI72
SMRiA2RI72
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 335ATP-graspUniRule annotationAdd BLAST204

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiYETKYTE

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A2RI72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKETLILLY GGRSAEREVS VLSAESVMRA VNYSRFIVKT YFITKGGEFI
60 70 80 90 100
KTQEFTDRPA EEEKLLTNDL AESYPKISPA AIYEKDAVVF PVLHGPMGED
110 120 130 140 150
GSIQGFLEIL RLAYVGPNIL SASSTMDKLL AKHVFEAVGV PQVPYVAAFA
160 170 180 190 200
DENQVEIAQE VVEKLDFPVF VKPANMGSSV GISKVDDLAD LQPALSEAYK
210 220 230 240 250
YDNRVVIEQG VDAREIECAV LGNNSDVSAT LPGEVVKDVG FYDYNSKYID
260 270 280 290 300
NKIQMDIPAK VSADLAQKIQ EYAKKAYKAV NGAGLSRCDF FVTKDGNVYL
310 320 330 340
NEVNAIPGFT QWSMYPLLWE NMGLSYSDLI EKLVDLAKET FETRENHLL
Length:349
Mass (Da):38,678
Last modified:March 6, 2007 - v1
Checksum:iE5453E652B2CE526
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406671 Genomic DNA Translation: CAL96965.1
RefSeqiWP_011834418.1, NC_009004.1

Genome annotation databases

EnsemblBacteriaiCAL96965; CAL96965; llmg_0360
KEGGillm:llmg_0360

Similar proteinsi

Entry informationi

Entry nameiDDL_LACLM
AccessioniPrimary (citable) accession number: A2RI72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health