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A2RI32

- AMPN_LACLM

UniProt

A2RI32 - AMPN_LACLM

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Protein
Aminopeptidase N
Gene
pepN, lap, llmg_0319
Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201Substrate By similarity
Metal bindingi288 – 2881Zinc; catalytic By similarity
Active sitei289 – 2891Proton acceptor By similarity
Metal bindingi292 – 2921Zinc; catalytic By similarity
Metal bindingi311 – 3111Zinc; catalytic By similarity
Sitei375 – 3751Transition state stabilizer By similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-332-MONOMER.

Protein family/group databases

MEROPSiM01.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name:
Lys-AP
Gene namesi
Name:pepN
Synonyms:lap
Ordered Locus Names:llmg_0319
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000000364: Chromosome

Subcellular locationi

Cytoplasm
Note: It may be secreted through an unknown mechanism.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 846845Aminopeptidase N
PRO_0000285237Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi416870.llmg_0319.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni252 – 2565Substrate binding By similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.

Phylogenomic databases

eggNOGiCOG0308.
HOGENOMiHOG000235375.
KOiK01256.
OMAiYLDINRE.
OrthoDBiEOG693GJ0.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2RI32-1 [UniParc]FASTAAdd to Basket

« Hide

MAVKRLIETF VPENYKIFLD IDRKTKKIKG QVAITGEAKD TVVSFHTKGL    50
HFNKVRAFSV DTNFIENEED EEIVVKIGET GRVTVSFEYE AELTDNMMGI 100
YPSYYEVNGE KKMLIGTQFE SHFARQAFPS IDEPEAKATF DLSVKFDEEE 150
GDIIVSNMPE LLNINGIHVF ERTVKMSSYL LAFVFGELQY KKGKTKSGVE 200
VGAFATKAHS QAALDFPLDI AIRSIEFYED YYQTPYPLPH SWHIALPDFS 250
AGAMENWGCI TYREVCMLVD PENATIQSKQ YVATVIAHEL AHQWFGDLVT 300
MQWWDDLWLN ESFANNMEYV CMDALEPSWN VWESFSISEA NMALNRDATD 350
GVQSVHVEVT HPDEIGTLFD PAIVYAKGSR LMVMLRKWLG DEDFAAGLAL 400
YFKRHQYGNT VGDNLWDALA EVSGKDVAAF MHSWVNQPGY PVVTAEVVDD 450
TLILSQKQFF VGEGVDKGRL WNVPLNTNWT GLPDLLSSEK VEIPGFAALK 500
TKNNGKALFL NDANMAHYII DYKGALLTDL LSEVESLENV TKFQILQDRK 550
LLAKAGVISY ADVVNILPSF TNEESYLVNT GLSQLISELE LFVDEDSETE 600
KAFQSLVGKL FAKNYARLGW DKVAGESAGD ESLRGIVLSK TLYSENADAK 650
TKASQIFAAH KENLASIPAD IRPIVLNNEI KTTNSAELVK TYRETYIKTS 700
LQEFKRELEG AVALIKDEKV IAELLESFKN ADIVKPQDIA FSWFYLLRND 750
FSQDAAWAWE KANWAFLEEK LGGDMSYDKF VIYPGNTFKT ADKLAEYKAF 800
FEPKLENQGL KRSIEMAIKQ ITARVALIDS QKAAVDKAIT DIAEKL 846
Length:846
Mass (Da):95,369
Last modified:March 6, 2007 - v1
Checksum:i3288DB1A43DBDEF8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87840 Genomic DNA. Translation: AAA25205.1.
S39955 Genomic DNA. Translation: AAB22460.1.
AM406671 Genomic DNA. Translation: CAL96925.1.
M65867 Genomic DNA. Translation: AAA25162.1.
PIRiS23157.
RefSeqiWP_011834382.1. NC_009004.1.
YP_001031673.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL96925; CAL96925; llmg_0319.
GeneIDi4797345.
KEGGillm:llmg_0319.
PATRICi22281679. VBILacLac4574_0328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87840 Genomic DNA. Translation: AAA25205.1 .
S39955 Genomic DNA. Translation: AAB22460.1 .
AM406671 Genomic DNA. Translation: CAL96925.1 .
M65867 Genomic DNA. Translation: AAA25162.1 .
PIRi S23157.
RefSeqi WP_011834382.1. NC_009004.1.
YP_001031673.1. NC_009004.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416870.llmg_0319.

Protein family/group databases

MEROPSi M01.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL96925 ; CAL96925 ; llmg_0319 .
GeneIDi 4797345.
KEGGi llm:llmg_0319.
PATRICi 22281679. VBILacLac4574_0328.

Phylogenomic databases

eggNOGi COG0308.
HOGENOMi HOG000235375.
KOi K01256.
OMAi YLDINRE.
OrthoDBi EOG693GJ0.

Enzyme and pathway databases

BioCyci LLAC416870:GCDT-332-MONOMER.

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the Lactococcus lactis pepN gene encoding an aminopeptidase homologous to mammalian aminopeptidase N."
    Tan P.S.T., van Alen-Boerrigter I.J., Poolman B., Siezen R.J., de Vos W.M., Konings W.N.
    FEBS Lett. 306:9-16(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-25.
  2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
    Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
    J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MG1363.
  3. "Characterization and overexpression of the Lactococcus lactis pepN gene and localization of its product, aminopeptidase N."
    van Alen-Boerrigter I.J., Baankreis R., de Vos W.M.
    Appl. Environ. Microbiol. 57:2555-2561(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167, CHARACTERIZATION.

Entry informationi

Entry nameiAMPN_LACLM
AccessioniPrimary (citable) accession number: A2RI32
Secondary accession number(s): P37897
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: March 6, 2007
Last modified: September 3, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi