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A2RI32

- AMPN_LACLM

UniProt

A2RI32 - AMPN_LACLM

Protein

Aminopeptidase N

Gene

pepN

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover.

    Catalytic activityi

    Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei120 – 1201SubstrateBy similarity
    Metal bindingi288 – 2881Zinc; catalyticPROSITE-ProRule annotation
    Active sitei289 – 2891Proton acceptorPROSITE-ProRule annotation
    Metal bindingi292 – 2921Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi311 – 3111Zinc; catalyticPROSITE-ProRule annotation
    Sitei375 – 3751Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW
    2. metallopeptidase activity Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciLLAC416870:GCDT-332-MONOMER.

    Protein family/group databases

    MEROPSiM01.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase N (EC:3.4.11.2)
    Alternative name(s):
    Alanine aminopeptidase
    Lysyl aminopeptidase
    Short name:
    Lys-AP
    Gene namesi
    Name:pepN
    Synonyms:lap
    Ordered Locus Names:llmg_0319
    OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
    Taxonomic identifieri416870 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000000364: Chromosome

    Subcellular locationi

    Cytoplasm
    Note: It may be secreted through an unknown mechanism.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 846845Aminopeptidase NPRO_0000285237Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi416870.llmg_0319.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni252 – 2565Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0308.
    HOGENOMiHOG000235375.
    KOiK01256.
    OMAiYLDINRE.
    OrthoDBiEOG693GJ0.

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A2RI32-1 [UniParc]FASTAAdd to Basket

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    MAVKRLIETF VPENYKIFLD IDRKTKKIKG QVAITGEAKD TVVSFHTKGL    50
    HFNKVRAFSV DTNFIENEED EEIVVKIGET GRVTVSFEYE AELTDNMMGI 100
    YPSYYEVNGE KKMLIGTQFE SHFARQAFPS IDEPEAKATF DLSVKFDEEE 150
    GDIIVSNMPE LLNINGIHVF ERTVKMSSYL LAFVFGELQY KKGKTKSGVE 200
    VGAFATKAHS QAALDFPLDI AIRSIEFYED YYQTPYPLPH SWHIALPDFS 250
    AGAMENWGCI TYREVCMLVD PENATIQSKQ YVATVIAHEL AHQWFGDLVT 300
    MQWWDDLWLN ESFANNMEYV CMDALEPSWN VWESFSISEA NMALNRDATD 350
    GVQSVHVEVT HPDEIGTLFD PAIVYAKGSR LMVMLRKWLG DEDFAAGLAL 400
    YFKRHQYGNT VGDNLWDALA EVSGKDVAAF MHSWVNQPGY PVVTAEVVDD 450
    TLILSQKQFF VGEGVDKGRL WNVPLNTNWT GLPDLLSSEK VEIPGFAALK 500
    TKNNGKALFL NDANMAHYII DYKGALLTDL LSEVESLENV TKFQILQDRK 550
    LLAKAGVISY ADVVNILPSF TNEESYLVNT GLSQLISELE LFVDEDSETE 600
    KAFQSLVGKL FAKNYARLGW DKVAGESAGD ESLRGIVLSK TLYSENADAK 650
    TKASQIFAAH KENLASIPAD IRPIVLNNEI KTTNSAELVK TYRETYIKTS 700
    LQEFKRELEG AVALIKDEKV IAELLESFKN ADIVKPQDIA FSWFYLLRND 750
    FSQDAAWAWE KANWAFLEEK LGGDMSYDKF VIYPGNTFKT ADKLAEYKAF 800
    FEPKLENQGL KRSIEMAIKQ ITARVALIDS QKAAVDKAIT DIAEKL 846
    Length:846
    Mass (Da):95,369
    Last modified:March 6, 2007 - v1
    Checksum:i3288DB1A43DBDEF8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87840 Genomic DNA. Translation: AAA25205.1.
    S39955 Genomic DNA. Translation: AAB22460.1.
    AM406671 Genomic DNA. Translation: CAL96925.1.
    M65867 Genomic DNA. Translation: AAA25162.1.
    PIRiS23157.
    RefSeqiWP_011834382.1. NC_009004.1.
    YP_001031673.1. NC_009004.1.

    Genome annotation databases

    EnsemblBacteriaiCAL96925; CAL96925; llmg_0319.
    GeneIDi4797345.
    KEGGillm:llmg_0319.
    PATRICi22281679. VBILacLac4574_0328.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87840 Genomic DNA. Translation: AAA25205.1 .
    S39955 Genomic DNA. Translation: AAB22460.1 .
    AM406671 Genomic DNA. Translation: CAL96925.1 .
    M65867 Genomic DNA. Translation: AAA25162.1 .
    PIRi S23157.
    RefSeqi WP_011834382.1. NC_009004.1.
    YP_001031673.1. NC_009004.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416870.llmg_0319.

    Protein family/group databases

    MEROPSi M01.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL96925 ; CAL96925 ; llmg_0319 .
    GeneIDi 4797345.
    KEGGi llm:llmg_0319.
    PATRICi 22281679. VBILacLac4574_0328.

    Phylogenomic databases

    eggNOGi COG0308.
    HOGENOMi HOG000235375.
    KOi K01256.
    OMAi YLDINRE.
    OrthoDBi EOG693GJ0.

    Enzyme and pathway databases

    BioCyci LLAC416870:GCDT-332-MONOMER.

    Family and domain databases

    InterProi IPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    Pfami PF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the Lactococcus lactis pepN gene encoding an aminopeptidase homologous to mammalian aminopeptidase N."
      Tan P.S.T., van Alen-Boerrigter I.J., Poolman B., Siezen R.J., de Vos W.M., Konings W.N.
      FEBS Lett. 306:9-16(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-25.
    2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
      Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
      J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MG1363.
    3. "Characterization and overexpression of the Lactococcus lactis pepN gene and localization of its product, aminopeptidase N."
      van Alen-Boerrigter I.J., Baankreis R., de Vos W.M.
      Appl. Environ. Microbiol. 57:2555-2561(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167, CHARACTERIZATION.

    Entry informationi

    Entry nameiAMPN_LACLM
    AccessioniPrimary (citable) accession number: A2RI32
    Secondary accession number(s): P37897
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3