A2RI32 (AMPN_LACLM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminopeptidase N
EC=3.4.11.2

Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name=Lys-AP

Gene names

Name:	pepN
Synonyms:	lap
Ordered Locus Names:	llmg_0319

Organism

Lactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]

Taxonomic identifier

416870 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	846 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Cytoplasm. Note: It may be secreted through an unknown mechanism.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 846	845	Aminopeptidase N		PRO_0000285237
Regions
	Region	252 – 256	5	Substrate binding By similarity
Sites
	Active site	289	1	Proton acceptor By similarity
	Metal binding	288	1	Zinc; catalytic By similarity
	Metal binding	292	1	Zinc; catalytic By similarity
	Metal binding	311	1	Zinc; catalytic By similarity
	Binding site	120	1	Substrate By similarity
	Site	375	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2RI32 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 3288DB1A43DBDEF8	FASTA	846	95,369
	10 20 30 40 50 60 MAVKRLIETF VPENYKIFLD IDRKTKKIKG QVAITGEAKD TVVSFHTKGL HFNKVRAFSV 70 80 90 100 110 120 DTNFIENEED EEIVVKIGET GRVTVSFEYE AELTDNMMGI YPSYYEVNGE KKMLIGTQFE 130 140 150 160 170 180 SHFARQAFPS IDEPEAKATF DLSVKFDEEE GDIIVSNMPE LLNINGIHVF ERTVKMSSYL 190 200 210 220 230 240 LAFVFGELQY KKGKTKSGVE VGAFATKAHS QAALDFPLDI AIRSIEFYED YYQTPYPLPH 250 260 270 280 290 300 SWHIALPDFS AGAMENWGCI TYREVCMLVD PENATIQSKQ YVATVIAHEL AHQWFGDLVT 310 320 330 340 350 360 MQWWDDLWLN ESFANNMEYV CMDALEPSWN VWESFSISEA NMALNRDATD GVQSVHVEVT 370 380 390 400 410 420 HPDEIGTLFD PAIVYAKGSR LMVMLRKWLG DEDFAAGLAL YFKRHQYGNT VGDNLWDALA 430 440 450 460 470 480 EVSGKDVAAF MHSWVNQPGY PVVTAEVVDD TLILSQKQFF VGEGVDKGRL WNVPLNTNWT 490 500 510 520 530 540 GLPDLLSSEK VEIPGFAALK TKNNGKALFL NDANMAHYII DYKGALLTDL LSEVESLENV 550 560 570 580 590 600 TKFQILQDRK LLAKAGVISY ADVVNILPSF TNEESYLVNT GLSQLISELE LFVDEDSETE 610 620 630 640 650 660 KAFQSLVGKL FAKNYARLGW DKVAGESAGD ESLRGIVLSK TLYSENADAK TKASQIFAAH 670 680 690 700 710 720 KENLASIPAD IRPIVLNNEI KTTNSAELVK TYRETYIKTS LQEFKRELEG AVALIKDEKV 730 740 750 760 770 780 IAELLESFKN ADIVKPQDIA FSWFYLLRND FSQDAAWAWE KANWAFLEEK LGGDMSYDKF 790 800 810 820 830 840 VIYPGNTFKT ADKLAEYKAF FEPKLENQGL KRSIEMAIKQ ITARVALIDS QKAAVDKAIT DIAEKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the Lactococcus lactis pepN gene encoding an aminopeptidase homologous to mammalian aminopeptidase N." Tan P.S.T., van Alen-Boerrigter I.J., Poolman B., Siezen R.J., de Vos W.M., Konings W.N. FEBS Lett. 306:9-16(1992) [PubMed: 1352755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-25.
[2]	"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363." Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J. J. Bacteriol. 189:3256-3270(2007) [PubMed: 17307855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MG1363.
[3]	"Characterization and overexpression of the Lactococcus lactis pepN gene and localization of its product, aminopeptidase N." van Alen-Boerrigter I.J., Baankreis R., de Vos W.M. Appl. Environ. Microbiol. 57:2555-2561(1991) [PubMed: 1685079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M87840 Genomic DNA. Translation: AAA25205.1. S39955 Genomic DNA. Translation: AAB22460.1. AM406671 Genomic DNA. Translation: CAL96925.1. M65867 Genomic DNA. Translation: AAA25162.1.
PIR	S23157.
RefSeq	YP_001031673.1. NC_009004.1.
3D structure databases
ProteinModelPortal	A2RI32.
ModBase	Search...
Protein-protein interaction databases
STRING	A2RI32.
Protein family/group databases
MEROPS	M01.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4797345.
GenomeReviews	Gene locus llmg_0319 in contig AM406671_GR.
KEGG	llm:llmg_0319.
PATRIC	22281679. VBILacLac4574_0328.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0308.
HOGENOM	HBG515894.
OMA	LIGTQFE.
ProtClustDB	CLSK876725.
Family and domain databases
InterPro	IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
KO	K01256.
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPN_LACLM

Accession

Primary (citable) accession number: A2RI32
Secondary accession number(s): P37897

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 6, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program