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A2RHZ5

- ACMA_LACLM

UniProt

A2RHZ5 - ACMA_LACLM

Protein

Probable N-acetylmuramidase

Gene

acmA

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    Hydrolyzes the cell wall of L.lactis and M.lysodeikticus. Required for cell separation during growth.

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    GO - Molecular functioni

    1. amidase activity Source: InterPro
    2. lysozyme activity Source: UniProtKB-EC

    GO - Biological processi

    1. barrier septum assembly Source: UniProtKB-KW
    2. cell wall macromolecule metabolic process Source: InterPro
    3. cytolysis Source: UniProtKB-KW
    4. defense response to bacterium Source: UniProtKB-KW
    5. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell cycle, Cell division, Cell wall biogenesis/degradation, Septation

    Enzyme and pathway databases

    BioCyciLLAC416870:GCDT-291-MONOMER.

    Protein family/group databases

    CAZyiCBM50. Carbohydrate-Binding Module Family 50.
    GH73. Glycoside Hydrolase Family 73.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable N-acetylmuramidase (EC:3.2.1.17)
    Alternative name(s):
    Autolysin
    Lysozyme
    Peptidoglycan hydrolase
    Gene namesi
    Name:acmA
    Ordered Locus Names:llmg_0280
    OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
    Taxonomic identifieri416870 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000000364: Chromosome

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 5757Sequence AnalysisAdd
    BLAST
    Chaini58 – 437380Probable N-acetylmuramidasePRO_0000285234Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi416870.llmg_0280.

    Structurei

    3D structure databases

    ProteinModelPortaliA2RHZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati245 – 28743LysM 1Add
    BLAST
    Repeati321 – 36343LysM 2Add
    BLAST
    Repeati395 – 43743LysM 3Add
    BLAST

    Domaini

    The LysM repeats are thought to be involved in peptidoglycan binding.

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 73 family.Curated
    Contains 3 LysM repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG1388.
    HOGENOMiHOG000033783.
    OMAiKAWRANA.
    OrthoDBiEOG6GTZPF.

    Family and domain databases

    Gene3Di3.10.350.10. 3 hits.
    InterProiIPR018392. LysM_dom.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view]
    PfamiPF01832. Glucosaminidase. 1 hit.
    PF01476. LysM. 3 hits.
    [Graphical view]
    SMARTiSM00257. LysM. 3 hits.
    SM00047. LYZ2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54106. SSF54106. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A2RHZ5-1 [UniParc]FASTAAdd to Basket

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    MPVSRVKVKN RHLKKKTKKP LAFYKPATKF AGAVLIAGTL TTTHELLLQQ    50
    TSPMVQAATN SSEVFIESIA ASAKPVADAN GLYPSVMIAQ AILESNWGSS 100
    QLSRAPYYNL FGIQGTYQGK SVVFKTQEYL NGKWVTKDMP FRVYPSFNQS 150
    FQDNAYVLKT TNFGNGPYYA KAWRANAATY QDATAALTGR YATDPSYGAS 200
    LNRIISQYNL TRFDGASSAG NTNSGGSTTT ITNNNSGTNS SSTTYTVKSG 250
    DTLWGISQRY GISVAQIQSA NNLKSTIIYI GQKLVLTGSA SSTNSGGSNN 300
    SASTTPTTSV TPAKPTSQTT VKVKSGDTLW ALSVKYKTSI AQLKSWNHLS 350
    SDTIYIGQNL IVSQSAAASN PSTGSGSTAT NNSNSTSSNS NASIHKVVKG 400
    DTLWGLSQKS GSPIASIKAW NHLSSDTILI GQYLRIK 437
    Length:437
    Mass (Da):46,564
    Last modified:March 6, 2007 - v1
    Checksum:i5C905633BD5DE28B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17696 Genomic DNA. Translation: AAC33367.1.
    AM406671 Genomic DNA. Translation: CAL96887.1.
    RefSeqiYP_001031636.1. NC_009004.1.

    Genome annotation databases

    EnsemblBacteriaiCAL96887; CAL96887; llmg_0280.
    GeneIDi4797197.
    KEGGillm:llmg_0280.
    PATRICi22281590. VBILacLac4574_0287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17696 Genomic DNA. Translation: AAC33367.1 .
    AM406671 Genomic DNA. Translation: CAL96887.1 .
    RefSeqi YP_001031636.1. NC_009004.1.

    3D structure databases

    ProteinModelPortali A2RHZ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416870.llmg_0280.

    Protein family/group databases

    CAZyi CBM50. Carbohydrate-Binding Module Family 50.
    GH73. Glycoside Hydrolase Family 73.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL96887 ; CAL96887 ; llmg_0280 .
    GeneIDi 4797197.
    KEGGi llm:llmg_0280.
    PATRICi 22281590. VBILacLac4574_0287.

    Phylogenomic databases

    eggNOGi COG1388.
    HOGENOMi HOG000033783.
    OMAi KAWRANA.
    OrthoDBi EOG6GTZPF.

    Enzyme and pathway databases

    BioCyci LLAC416870:GCDT-291-MONOMER.

    Family and domain databases

    Gene3Di 3.10.350.10. 3 hits.
    InterProi IPR018392. LysM_dom.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view ]
    Pfami PF01832. Glucosaminidase. 1 hit.
    PF01476. LysM. 3 hits.
    [Graphical view ]
    SMARTi SM00257. LysM. 3 hits.
    SM00047. LYZ2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54106. SSF54106. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation."
      Buist G., Kok J., Leenhouts K.J., Dabrowska M., Venema G., Haandrikman A.J.
      J. Bacteriol. 177:1554-1563(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
      Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
      J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MG1363.

    Entry informationi

    Entry nameiACMA_LACLM
    AccessioniPrimary (citable) accession number: A2RHZ5
    Secondary accession number(s): O52362, Q48603
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3