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A2RHZ5 (ACMA_LACLM) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable N-acetylmuramidase
EC=3.2.1.17
Alternative name(s):
Autolysin
Lysozyme
Peptidoglycan hydrolase
Gene names
Name:acmA
Ordered Locus Names:llmg_0280
OrganismLactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]
Taxonomic identifier416870 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Hydrolyzes the cell wall of L.lactis and M.lysodeikticus. Required for cell separation during growth.

Catalytic activity

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Subcellular location

Secreted By similarity.

Domain

The LysM repeats are thought to be involved in peptidoglycan binding.

Sequence similarities

Belongs to the glycosyl hydrolase 73 family.

Contains 3 LysM repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5757 Potential
Chain58 – 437380Probable N-acetylmuramidase
PRO_0000285234

Regions

Repeat245 – 28743LysM 1
Repeat321 – 36343LysM 2
Repeat395 – 43743LysM 3

Sequences

Sequence LengthMass (Da)Tools
A2RHZ5 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 5C905633BD5DE28B

FASTA43746,564
        10         20         30         40         50         60 
MPVSRVKVKN RHLKKKTKKP LAFYKPATKF AGAVLIAGTL TTTHELLLQQ TSPMVQAATN 

        70         80         90        100        110        120 
SSEVFIESIA ASAKPVADAN GLYPSVMIAQ AILESNWGSS QLSRAPYYNL FGIQGTYQGK 

       130        140        150        160        170        180 
SVVFKTQEYL NGKWVTKDMP FRVYPSFNQS FQDNAYVLKT TNFGNGPYYA KAWRANAATY 

       190        200        210        220        230        240 
QDATAALTGR YATDPSYGAS LNRIISQYNL TRFDGASSAG NTNSGGSTTT ITNNNSGTNS 

       250        260        270        280        290        300 
SSTTYTVKSG DTLWGISQRY GISVAQIQSA NNLKSTIIYI GQKLVLTGSA SSTNSGGSNN 

       310        320        330        340        350        360 
SASTTPTTSV TPAKPTSQTT VKVKSGDTLW ALSVKYKTSI AQLKSWNHLS SDTIYIGQNL 

       370        380        390        400        410        420 
IVSQSAAASN PSTGSGSTAT NNSNSTSSNS NASIHKVVKG DTLWGLSQKS GSPIASIKAW 

       430 
NHLSSDTILI GQYLRIK

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation."
Buist G., Kok J., Leenhouts K.J., Dabrowska M., Venema G., Haandrikman A.J.
J. Bacteriol. 177:1554-1563(1995) [PubMed: 7883712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
J. Bacteriol. 189:3256-3270(2007) [PubMed: 17307855] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MG1363.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U17696 Genomic DNA. Translation: AAC33367.1.
AM406671 Genomic DNA. Translation: CAL96887.1.
RefSeqYP_001031636.1. NC_009004.1.

3D structure databases

ProteinModelPortalA2RHZ5.
ModBaseSearch...

Protein-protein interaction databases

STRINGA2RHZ5.

Protein family/group databases

CAZyCBM50. Carbohydrate-Binding Module Family 50.
GH73. Glycoside Hydrolase Family 73.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4797197.
GenomeReviewsGene locus llmg_0280 in contig AM406671_GR.
KEGGllm:llmg_0280.
PATRIC22281590. VBILacLac4574_0287.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1705.
HOGENOMHBG726129.
OMATSIAKAY.
ProtClustDBCLSK2567551.

Family and domain databases

InterProIPR000423. Flag_FlgJ_1.
IPR013338. Lysozyme_dom_subfam2.
IPR002901. Mano_Glyc_endo_b_GlcNAc.
IPR018392. Peptidoglycan-bd_lysin.
IPR002482. Peptidoglycan-bd_Lysin_subgr.
[Graphical view]
PfamPF01832. Glucosaminidase. 1 hit.
PF01476. LysM. 3 hits.
[Graphical view]
PRINTSPR01002. FLGFLGJ.
SMARTSM00257. LysM. 3 hits.
SM00047. LYZ2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACMA_LACLM
AccessionPrimary (citable) accession number: A2RHZ5
Secondary accession number(s): O52362, Q48603
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: March 6, 2007
Last modified: December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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