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A2RF20 (TYSY_STRPG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:SpyM51120
OrganismStreptococcus pyogenes serotype M5 (strain Manfredo) [Complete proteome] [HAMAP]
Taxonomic identifier160491 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Thymidylate synthase HAMAP-Rule MF_00008
PRO_1000000693

Sites

Active site1541 By similarity

Sequences

Sequence LengthMass (Da)Tools
A2RF20 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 6D8B39FA881D4B8D

FASTA27932,564
        10         20         30         40         50         60 
MTKADQIFKA NIQKIINEGS LSEQARPKYK DGRTAHSKYI TGAFAEYDLA KGEFPITTLR 

        70         80         90        100        110        120 
PIPIKSAIKE LFWIYQDQSN SLDVLEAKYN VHYWNEWEVD QTRTIGQRYG AVVKKHDIIS 

       130        140        150        160        170        180 
KILKQLAENP WNRRNVISLW DYEAFEETKG LLPCAFQIMF DVRRVGEDLY LDASLTQRSN 

       190        200        210        220        230        240 
DILVAHHINA MQYVALQMMI AKHFGWKIGK FFYFVNNLHI YDNQFDQAQE LLKRQPVASQ 

       250        260        270 
PKLVLNVPDG TNFFDIKPDD FELQNYDPVK PQLHFDLAI 

« Hide

References

[1]"Complete genome of acute rheumatic fever-associated serotype M5 Streptococcus pyogenes strain Manfredo."
Holden M.T.G., Scott A., Cherevach I., Chillingworth T., Churcher C., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S., Mungall K., Quail M.A., Price C., Rabbinowitsch E., Sharp S., Skelton J. expand/collapse author list , Whitehead S., Barrell B.G., Kehoe M., Parkhill J.
J. Bacteriol. 189:1473-1477(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Manfredo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM295007 Genomic DNA. Translation: CAM30446.1.
RefSeqYP_001128669.1. NC_009332.1.

3D structure databases

ProteinModelPortalA2RF20.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160491.SpyM51120.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4964092.
KEGGspf:SpyM51120.
PATRIC19765422. VBIStrPyo41547_1163.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257898.
KOK00560.
OMATIGHAYG.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycSPYO160491:GJ8O-1176-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_STRPG
AccessionPrimary (citable) accession number: A2RF20
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 6, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways