A2RD38 (PROA_STRPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	SpyM50421

Organism

Streptococcus pyogenes serotype M5 (strain Manfredo) [Complete proteome] [HAMAP]

Taxonomic identifier

160491 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049996

Sequences

	Sequence		Length	Mass (Da)
	A2RD38 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: DDDDE9DF33D9D4BD	FASTA	416	45,407
	10 20 30 40 50 60 MTDMRRLGQR AKQASLLIAP LSTQIKNRFL STLAKALVDD TQTLLAANQK DLANAKEHGI 70 80 90 100 110 120 SDIMMDRLRL TSERIKAIAQ GVQQVADLAD PIGQVIKGYT NLDGLKILQK RVPLGVIAMI 130 140 150 160 170 180 FESRPNVSVD AFSLAFKTNN AIILRGGKDA LHSNKALVKL IRQSLEKSGI TPDAVQLVED 190 200 210 220 230 240 PSHAVAEELM QATDYVDVLI PRGGAKLIQT VKEKAKVPVI ETGVGNVHIY VDAQADLDMA 250 260 270 280 290 300 TNIVINAKTK RPSVCNAAEG LVIHEAVAAR FIPMLEKAIN QVQPVEWRAD DKALPLFEQA 310 320 330 340 350 360 VPAKAEDFET EFLDYIMSVK VVSSLEEAIS WINQHTSHHS EAIITRDIKA AETFQDLVDA 370 380 390 400 410 AAVYVNASTR FTDGFVFGLG AEIGISTQKM HARGPMGLEA LTSTKFYING DGHIRE

References

[1]

"Complete genome of acute rheumatic fever-associated serotype M5 Streptococcus pyogenes strain Manfredo." Holden M.T.G., Scott A., Cherevach I., Chillingworth T., Churcher C., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S., Mungall K., Quail M.A., Price C., Rabbinowitsch E., Sharp S., Skelton J. , Whitehead S., Barrell B.G., Kehoe M., Parkhill J. J. Bacteriol. 189:1473-1477(2007) [PubMed: 17012393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Manfredo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM295007 Genomic DNA. Translation: CAM29763.1.
RefSeq	YP_001128008.1. NC_009332.1.
3D structure databases
ProteinModelPortal	A2RD38.
ModBase	Search...
Protein-protein interaction databases
STRING	A2RD38.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000047818; EBSTRP00000045946; EBSTRG00000047810.
GeneID	4963003.
GenomeReviews	Gene locus SpyM50421 in contig AM295007_GR.
KEGG	spf:SpyM50421.
PATRIC	19763968. VBIStrPyo41547_0451.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
GeneTree	EBGT00050000027638.
HOGENOM	HBG318080.
OMA	QYPAACN.
ProtClustDB	PRK00197.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_STRPG

Accession

Primary (citable) accession number: A2RD38

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 6, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program