A2RA94 (ALG3_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
EC=2.4.1.258

Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase

Gene names

Name:	alg3
ORF Names:	An18g02360

Organism

Aspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]

Taxonomic identifier

425011 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase		PRO_0000350922
Regions
	Topological domain	1 – 18	18	Lumenal Potential
	Transmembrane	19 – 39	21	Helical; Potential
	Topological domain	40 – 59	20	Cytoplasmic Potential
	Transmembrane	60 – 82	23	Helical; Potential
	Topological domain	83 – 91	9	Lumenal Potential
	Transmembrane	92 – 112	21	Helical; Potential
	Topological domain	113 – 137	25	Cytoplasmic Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Topological domain	159 – 167	9	Lumenal Potential
	Transmembrane	168 – 190	23	Helical; Potential
	Topological domain	191 – 200	10	Cytoplasmic Potential
	Transmembrane	201 – 219	19	Helical; Potential
	Topological domain	220 – 257	38	Lumenal Potential
	Transmembrane	258 – 278	21	Helical; Potential
	Topological domain	279 – 302	24	Cytoplasmic Potential
	Transmembrane	303 – 323	21	Helical; Potential
	Topological domain	324 – 335	12	Lumenal Potential
	Transmembrane	336 – 356	21	Helical; Potential
	Topological domain	357 – 373	17	Cytoplasmic Potential
	Transmembrane	374 – 394	21	Helical; Potential
	Topological domain	395 – 409	15	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	A2RA94 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 06B424D9C347896F	FASTA	409	45,943
	10 20 30 40 50 60 MDWMRLIRDL CFNPRHTKWM APLLVLGDAF LCALIIWKVP CKATAKLRSH PFATRERDYT 70 80 90 100 110 120 LIRGSTGPLV YPAAHVYSYT ALYHLTDEGR DIFFGQILFA VLYLITLVVV LCCYRQSGAP 130 140 150 160 170 180 PYLLPLLVLS KRLHSVYVLR LFNDGLAALA MWVAILLFMN RKWTAAVAVW STGVAIKMTL 190 200 210 220 230 240 LLLAPAIAVV TVLSLSLGPS VGLGVLAVLV QVLLAIPFLQ NNPAGYLSRA FELTRQFMFK 250 260 270 280 290 300 WTVNWRFVGE EVFLSKSFSL ALLAVHIVLL GAFAVTGWLR YSRSSLPAFI RNLLAGRHRT 310 320 330 340 350 360 VSLPKPYIMS VMLSSLTVGL LCARSLHYQF FAYLSWATPF LLWRAGFHPI LLYLIWAMQE 370 380 390 400 WAWNTFPSTN LSSIIVVLSL ATQSFGVLAN SASAFYTMRS NPSGKEHNQ

References

[1]

"Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88." Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M. , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H. Nat. Biotechnol. 25:221-231(2007) [PubMed: 17259976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CBS 513.88 / FGSC A1513.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM270400 Genomic DNA. Translation: CAK43166.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT58. Glycosyltransferase Family 58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANGAT00013870; CADANGAP00013614; CADANGAG00013870.
GenomeReviews	Gene locus alg3 in contig AM270997_GR.
Phylogenomic databases
GeneTree	EFGT00050000003598.
HOGENOM	HBG525447.
OrthoDB	EOG4MPN04.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_ASPNC

Accession

Primary (citable) accession number: A2RA94

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	March 6, 2007
Last modified:	September 21, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program