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Protein

Probable endopolygalacturonase A

Gene

pgaA

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall (By similarity).By similarity

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei207Proton donorPROSITE-ProRule annotation1
Active sitei229PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endopolygalacturonase A (EC:3.2.1.15)
Alternative name(s):
Pectinase A
Polygalacturonase A
Gene namesi
Name:pgaA
Synonyms:pecA
ORF Names:An16g06990
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 5R

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000039364120 – 32Sequence analysisAdd BLAST13
ChainiPRO_500022121533 – 370Probable endopolygalacturonase AAdd BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi35 ↔ 50By similarity
Disulfide bondi209 ↔ 225By similarity
Glycosylationi246N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi335 ↔ 340By similarity
Disulfide bondi359 ↔ 368By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliA2R8F8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati162 – 192PbH1 1Add BLAST31
Repeati193 – 214PbH1 2Add BLAST22
Repeati215 – 235PbH1 3Add BLAST21
Repeati244 – 265PbH1 4Add BLAST22
Repeati273 – 295PbH1 5Add BLAST23
Repeati307 – 352PbH1 6Add BLAST46

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000193107.
OrthoDBiEOG092C33Y3.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2R8F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSAKPLFCL ATLAGAALAA PAPSRVSDFT KRSTCTFTDA ATASESKTSC
60 70 80 90 100
SDIVLKDITV PAGETLNLKD LNDGTTVTFE GTTTWEYEEW DGPLLRISGK
110 120 130 140 150
DITVTQSSDA VLDGNGAKWW DGEGTNGGKT KPKFFYAHDL DDSKISGLYI
160 170 180 190 200
KNTPVQAISV ESDNLVIEDV TIDNSDGDSE GGHNTDGFDI SESTYITITG
210 220 230 240 250
ATVKNQDDCV AINSGENIYF SGGTCSGGHG LSIGSVGGRD DNTVKNVTFI
260 270 280 290 300
DSTVSDSENG VRIKTVYDAT GTVEDITYSN IQLSGISDYG IVIEQDYENG
310 320 330 340 350
DPTGTPSNGV TISDVTLEDI TGSVDSDAVE IYILCGDGSC SDWTMSGIDI
360 370
TGGETSSDCE NVPSGASCDQ
Length:370
Mass (Da):38,735
Last modified:September 23, 2008 - v1
Checksum:i158C187F95078BB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270374 Genomic DNA. Translation: CAK47020.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270374 Genomic DNA. Translation: CAK47020.1.

3D structure databases

ProteinModelPortaliA2R8F8.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOGENOMiHOG000193107.
OrthoDBiEOG092C33Y3.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLRA_ASPNC
AccessioniPrimary (citable) accession number: A2R8F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: September 23, 2008
Last modified: November 2, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.