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Protein

Probable alpha-L-arabinofuranosidase B

Gene

abfB

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan (By similarity).By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathwayi: L-arabinan degradation

This protein is involved in the pathway L-arabinan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway L-arabinan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei176 – 177Cis-peptide bondBy similarity2
Binding sitei219SubstrateBy similarity1
Active sitei221NucleophileBy similarity1
Binding sitei222Substrate; via amide nitrogenBy similarity1
Binding sitei223Substrate; via amide nitrogenBy similarity1
Binding sitei296Substrate; via amide nitrogenBy similarity1
Active sitei297Proton donorBy similarity1
Binding sitei416SubstrateBy similarity1
Binding sitei418Substrate; via amide nitrogenBy similarity1
Binding sitei419Substrate; via amide nitrogenBy similarity1
Binding sitei435SubstrateBy similarity1
Binding sitei463SubstrateBy similarity1
Binding sitei465Substrate; via amide nitrogenBy similarity1
Binding sitei468Substrate; via amide nitrogenBy similarity1
Binding sitei488SubstrateBy similarity1

GO - Molecular functioni

  • alpha-L-arabinofuranosidase activity Source: AspGD

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00667

Protein family/group databases

CAZyiCBM42 Carbohydrate-Binding Module Family 42
GH54 Glycoside Hydrolase Family 54

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-arabinofuranosidase B (EC:3.2.1.55)
Short name:
ABF B
Short name:
Arabinosidase B
Gene namesi
Name:abfB
ORF Names:An15g02300
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 3R

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_500022102419 – 499Probable alpha-L-arabinofuranosidase BAdd BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi21 ↔ 31By similarity
Disulfide bondi81 ↔ 86By similarity
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi176 ↔ 177By similarity
Glycosylationi202N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi401 ↔ 439By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiA2R511

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00011686

Structurei

3D structure databases

ProteinModelPortaliA2R511
SMRiA2R511
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 335CatalyticBy similarityAdd BLAST317
Regioni336 – 499ABDBy similarityAdd BLAST164

Domaini

Organized into two domains: an N-terminal catalytic domain and a C-terminal arabinose-binding domain (ABD).By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 54 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000187007
KOiK20844
OrthoDBiEOG092C3Z3T

Family and domain databases

InterProiView protein in InterPro
IPR015289 A-L-arabinofuranosidase_B_cat
IPR038964 ABFB
IPR007934 AbfB_ABD
IPR036195 AbfB_ABD_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR39447 PTHR39447, 1 hit
PfamiView protein in Pfam
PF05270 AbfB, 1 hit
PF09206 ArabFuran-catal, 1 hit
SUPFAMiSSF110221 SSF110221, 1 hit
SSF49899 SSF49899, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2R511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSRRNLVAL GLAATVSAGP CDIYEAGDTP CVAAHSTTRA LYSSFSGALY
60 70 80 90 100
QLQRGSDDTT TTISPLTAGG VADASAQDTF CANTTCLITI IYDQSGNGNH
110 120 130 140 150
LTQAPPGGFD GPDVDGYDNL ASAIGAPVTL NGQKAYGVFM SPGTGYRNNE
160 170 180 190 200
ATGTATGDEP EGMYAVLDGT HYNDACCFDY GNAETSSTDT GAGHMEAIYL
210 220 230 240 250
GNSTTWGYGA GDGPWIMVDM ENNLFSGADE GYNSGDPSIS YSFVTAAVKG
260 270 280 290 300
GADKWAIRGG NAASGSLSTY YSGARPDYSG YNPMSKEGAI ILGIGGDNSN
310 320 330 340 350
GAQGTFYEGV MTSGYPSDDV ENSVQENIVA AKYVSGSLVS GPSFTSGEVV
360 370 380 390 400
SLRVTTPGYT TRYIAHTDTT VNTQVVDDDS STTLKEEASW TVVTGLANSQ
410 420 430 440 450
CFSFESVDTP GSYIRHYNFE LLLNANDGTK QFHEDATFCP QAPLNGEGTS
460 470 480 490
LRSWSYPTRY FRHYDNVLYA ASNGGVQTFD SKTSFNNDVS FEIETAFAS
Length:499
Mass (Da):52,509
Last modified:March 6, 2007 - v1
Checksum:iF1F6C21F601419C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270337 Genomic DNA Translation: CAK42333.1
RefSeqiXP_001396769.1, XM_001396732.2

Genome annotation databases

EnsemblFungiiCAK42333; CAK42333; An15g02300
GeneIDi4987831
KEGGiang:ANI_1_372134

Similar proteinsi

Entry informationi

Entry nameiABFB_ASPNC
AccessioniPrimary (citable) accession number: A2R511
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 6, 2007
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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