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Protein

Aspartic protease pep1

Gene

pep1

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. Can catalyze hydrolysis of the major structural proteins of basement membrane, elastin, collagen, and laminin (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins with broad specificity. Generally favors hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101PROSITE-ProRule annotation1
Active sitei283PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic protease pep1 (EC:3.4.23.18)
Alternative name(s):
Aspergillopepsin A
Aspergillopepsin I
Gene namesi
Name:pep1
Synonyms:pepA
ORF Names:An14g04710
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 1R

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000040704521 – 69Activation peptideBy similarityAdd BLAST49
ChainiPRO_500022096570 – 394Aspartic protease pep1Add BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi319 ↔ 354By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliA2R3L3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 391Peptidase A1PROSITE-ProRule annotationAdd BLAST307

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000167484.
KOiK06004.
OrthoDBiEOG092C49YE.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2R3L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVFSKTAAL VLGLSSAVSA APAPTRKGFT INQIARPANK TRTINLPGMY
60 70 80 90 100
ARSLAKFGGT VPQSVKEAAS KGSAVTTPQN NDEEYLTPVT VGKSTLHLDF
110 120 130 140 150
DTGSADLWVF SDELPSSEQT GHDLYTPSSS ATKLSGYTWD ISYGDGSSAS
160 170 180 190 200
GDVYRDTVTV GGVTTNKQAV EAASKISSEF VQNTANDGLL GLAFSSINTV
210 220 230 240 250
QPKAQTTFFD TVKSQLDSPL FAVQLKHDAP GVYDFGYIDD SKYTGSITYT
260 270 280 290 300
DADSSQGYWG FSTDGYSIGD GSSSSSGFSA IADTGTTLIL LDDEIVSAYY
310 320 330 340 350
EQVSGAQESE EAGGYVFSCS TNPPDFTVVI GDYKAVVPGK YINYAPISTG
360 370 380 390
SSTCFGGIQS NSGLGLSILG DVFLKSQYVV FNSEGPKLGF AAQA
Length:394
Mass (Da):41,276
Last modified:March 6, 2007 - v1
Checksum:i68874D5424F9128F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270324 Genomic DNA. Translation: CAK42031.1.
RefSeqiXP_001401093.1. XM_001401056.2.

Genome annotation databases

EnsemblFungiiCAK42031; CAK42031; An14g04710.
GeneIDi4987328.
KEGGiang:ANI_1_654124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270324 Genomic DNA. Translation: CAK42031.1.
RefSeqiXP_001401093.1. XM_001401056.2.

3D structure databases

ProteinModelPortaliA2R3L3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA01.016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAK42031; CAK42031; An14g04710.
GeneIDi4987328.
KEGGiang:ANI_1_654124.

Phylogenomic databases

HOGENOMiHOG000167484.
KOiK06004.
OrthoDBiEOG092C49YE.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA_ASPNC
AccessioniPrimary (citable) accession number: A2R3L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: March 6, 2007
Last modified: November 30, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.