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Protein

Lysophospholipase nte1

Gene

nte1

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by organophosphorus esters.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1261 – 12611By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi689 – 809121cNMP 1Add
BLAST
Nucleotide bindingi849 – 969121cNMP 2Add
BLAST

GO - Molecular functioni

  1. cAMP-dependent protein kinase regulator activity Source: ASPGD
  2. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. asexual sporulation resulting in formation of a cellular spore Source: ASPGD
  2. lipid catabolic process Source: UniProtKB-KW
  3. positive regulation of asexual sporulation resulting in formation of a cellular spore Source: ASPGD
  4. regulation of protein kinase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase nte1 (EC:3.1.1.5)
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene namesi
Name:nte1
ORF Names:An14g03050
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 1R

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7272CytoplasmicBy similarityAdd
BLAST
Transmembranei73 – 9321HelicalSequence AnalysisAdd
BLAST
Topological domaini94 – 11522LumenalBy similarityAdd
BLAST
Transmembranei116 – 13621HelicalSequence AnalysisAdd
BLAST
Topological domaini137 – 15311395CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular Source: ASPGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15311531Lysophospholipase nte1PRO_0000295311Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00011022.

Structurei

3D structure databases

ProteinModelPortaliA2R350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1228 – 1392165PatatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1259 – 12635GXSXG

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi58 – 614Poly-Pro

Sequence similaritiesi

Belongs to the NTE family.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 patatin domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000048680.
OrthoDBiEOG70W3NS.

Family and domain databases

Gene3Di2.60.120.10. 5 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 5 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2R350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATGDGIIAA PPSLESSSLD PLHVLPASSS TAARSLATSI PALTASFSVV
60 70 80 90 100
SGFSSHLPPP PVTPPAPSTM VGWIGWIFSF IFQVIPSVLY WIVTFTTITL
110 120 130 140 150
PTWLFTLFSM SLTFTMNFTT LLLIALAVVS TISWFIRYRF LNMYSRLPPE
160 170 180 190 200
PQRKEPQLDL FPDVPDSDTK PGLANYLDEF LSAIKVFGYL ERPVFHELTR
210 220 230 240 250
TMQTRKLIAG ETLLLEEEKG FCLVVDGLVQ IFVKSMRDRK SGSDEELNRM
260 270 280 290 300
AGESSDEDDH RPDGRQGYQL LTEVKNGASM SSLFSILSLF TEDVQLRYAD
310 320 330 340 350
SSASSASSIG PGLAIGPDSF PASPREMDDS PHVYQGDRLD PASHRNSTAE
360 370 380 390 400
DLPAVPPLNL GESQVPPAHS ARSESRKYSG KTRRKSVHPD IVARAMVDTT
410 420 430 440 450
IAIIPASAFR RLTRVYPKAT AHIVQVILTR LQRVTFATAH SYLGLNNEVL
460 470 480 490 500
GIEKQMTKFT TYDLPNELRG SALDRLKDKF IKERDRLGQE EVTKGIALHN
510 520 530 540 550
PYGGRRPRSS SFRRKEAALQ AKMVASKRPV SMVAQDSALS DRENSGVSPG
560 570 580 590 600
DLLSTIQLSR FGPRYEHLAP RLLSPLTEKE HSPLRSPSPM IPGRASPFHR
610 620 630 640 650
KESLDEDALF RESILECIMK GIGLTGSTND FLRKSSHPSG DVSPKLLSYD
660 670 680 690 700
SRRQKAVFSN NAFGFIDPYE GSADGESESM MSMSVTSAGG TSPIVNLREE
710 720 730 740 750
LRNDIEIVYF PQGSVLVEQG ERHPGLYYVV DGFLDVGIPV DDKEEDLVGS
760 770 780 790 800
SRPAHEELFP MLRRTNTSSS RVSGSAAAAN DPRRKKQSRR SLYLIKPGGI
810 820 830 840 850
QGYVGAVASY RSYTDVVAKT DVYVGFLPRA SLERIAERYP LALLTLAKRL
860 870 880 890 900
TSLLPRLLLH IDFALEWVQV SAGQVIYHQG DESDAIYLAL NGRLRSVHEG
910 920 930 940 950
PNGKMTVVGE YGQGESVGEL EVMTESTRPA TLHAIRDTEL AKFPRTLFNS
960 970 980 990 1000
LAQEHPGITI QVSKLIAQRM RDLVETPLAE KGGEPGVSGT VKTAKSTLNL
1010 1020 1030 1040 1050
RTVGILPVTA GVPVVEFGNR LLQALHQIGV TNGATSLNQA AILNHLGRHA
1060 1070 1080 1090 1100
FSKMGKLKLS QYLADLEEKY GMVLYIADTS VNSPWTQTCI TQADCILLVG
1110 1120 1130 1140 1150
LAESTPSIGE YERFLLGMKT TARKELVLLH GERYCPPGLT RQWLKNRVWI
1160 1170 1180 1190 1200
NGGHHHVQMA FRLTAEPSHP QTKRFGTVLK QRVQVIQAEI QKYTSRRIRQ
1210 1220 1230 1240 1250
SPLYSAQTPF KGDFHRLARR LCGRSVGLVL GGGGARGIAQ VGVIKALEEA
1260 1270 1280 1290 1300
GIPIDVIGGT SIGSFIGALY ARDADVVPMY GRAKKFAGRM GSIWRFALDL
1310 1320 1330 1340 1350
TYPTVSYTTG HEFNRGIFKT FGDSQIEDFW LEFYCNTTNI SRSRAEYHSS
1360 1370 1380 1390 1400
GYTWRYVRAS MSLAGLIPPI CDEGSMLLDG GYIDNLTVDH MKGLGADVIF
1410 1420 1430 1440 1450
AVDVGSIDDN TPQVYGDSLS GFWAVVNRWN PFSSCPNPPT LSEIQARLAY
1460 1470 1480 1490 1500
VSSIENLERA KNTPGCLYMR PPIDPYGTLD FAKFDEIYQL GYAYGKNYLE
1510 1520 1530
KLKREGSLPL PEETEEKKKL QRTLAPRRAS I
Length:1,531
Mass (Da):169,000
Last modified:July 24, 2007 - v2
Checksum:i2145CB24C8D6CE79
GO

Sequence cautioni

The sequence CAK46542.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270320 Genomic DNA. Translation: CAK46542.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270320 Genomic DNA. Translation: CAK46542.1. Sequence problems.

3D structure databases

ProteinModelPortaliA2R350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5061.CADANGAP00011022.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000048680.
OrthoDBiEOG70W3NS.

Family and domain databases

Gene3Di2.60.120.10. 5 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 5 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiNTE1_ASPNC
AccessioniPrimary (citable) accession number: A2R350
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: January 7, 2015
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.