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A2R345 (DCL21_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dicer-like protein 2-1

Including the following 2 domains:

  1. Endoribonuclease dcl2-1
    EC=3.1.26.-
  2. ATP-dependent helicase dcl2-1
    EC=3.6.4.-
Gene names
Name:dcl2-1
ORF Names:An14g03000
OrganismAspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]
Taxonomic identifier425011 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1387 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.

Cofactor

Magnesium or manganese By similarity.

Sequence similarities

Belongs to the helicase family. Dicer subfamily.

Contains 1 Dicer dsRNA-binding fold domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 2 RNase III domains.

Sequence caution

The sequence CAK46537.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13871387Dicer-like protein 2-1
PRO_0000306787

Regions

Domain26 – 205180Helicase ATP-binding
Domain370 – 535166Helicase C-terminal
Domain565 – 65995Dicer dsRNA-binding fold
Domain915 – 1055141RNase III 1
Domain1094 – 1277184RNase III 2
Nucleotide binding39 – 468ATP By similarity
Motif146 – 1494DEAH box

Sites

Metal binding11331Magnesium or manganese By similarity
Metal binding12631Magnesium or manganese By similarity
Metal binding12661Magnesium or manganese By similarity
Site12591Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A2R345 [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: 68B17C1A26553265

FASTA1,387156,456
        10         20         30         40         50         60 
MTVAATVLPA GEDAPAYRPR SYQVEMFEAS LKENIIVTMG TGSGKTHIAL LRIIKELESN 

        70         80         90        100        110        120 
PHKLIWFLTP TVALCLQQFK FLSDNIPAVR ARTLTSLDKV ELWTEQPVWD AILKEMQVVV 

       130        140        150        160        170        180 
STHAVLADAM SHGFVKITQL GLMIFDEAHH CMRRHPANKI MQDFYHPALE RHGAEAVPKI 

       190        200        210        220        230        240 
LGLTASPVVR SNRQELLKIE SNLDAVCKTP RTHRSELMTH THRPHLQQIL FTPVLLDDLQ 

       250        260        270        280        290        300 
VGSKTLKALV SAWTSLRLED DPYIKKLRKS PLDGRALQKV LESGKTYCND QLKRFATRSL 

       310        320        330        340        350        360 
HIFEELGEWA ADYFIHASIE QLKARAGNSA DTMGWTDEEK AYLLDIVSKL PIPNIDLTHS 

       370        380        390        400        410        420 
DPDRIPISSK FRSLLEFLDT KGEPNFSGLI FAKQRATVSV MEKLLSIHPV TKHRFRCASF 

       430        440        450        460        470        480 
VGWSGGGSKD VLGELLDARM QRDTLSEFRT GQKNLIIATD VLEEGIDISA CSVVVCFDKP 

       490        500        510        520        530        540 
PNLKSFVQRR GRARHRQSTY AIMFATDDES SALSKWEDLE QAMIEAYEDD ERRLREAWAL 

       550        560        570        580        590        600 
EAINEEVVER LEVQSTGAVL TADTAVAHLN HFCAVLPRQP YASNEPEFSY EKDDADLLRG 

       610        620        630        640        650        660 
TVTLPSCVHP GVRRIQGQRW WQTERAARKE AAFQAYKRLY EFGLLSDHLL PFKRNLELKE 

       670        680        690        700        710        720 
TDLTNLPALV EVSEQYDPWV DWACSWSSPD VHQTRIAIKH NGDSRMCIRL TSPTSLPPVE 

       730        740        750        760        770        780 
PMTLFWDSET IYTLDFDKPK RMKEIAAESI ENMRLATALY LQAASSRQMR PEQDFVTLFG 

       790        800        810        820        830        840 
PDLTDLELAE WLNKHAGDEP ALEVYSRKDF PTVMGIVRDR SRYNEPMLFK RWVVSGQDDT 

       850        860        870        880        890        900 
PIVELECDAV PKRRNLLHRQ TLAAKQPDSE TPAISSKIRL ILAENCTIDK LPYAETIFGR 

       910        920        930        940        950        960 
FISVILDRLE ATLVATRLCE TILRDLEFSS IRHIITAITA PSAQSLTNYQ RYEFFGDSVL 

       970        980        990       1000       1010       1020 
KFTVSCQLFF QHPNWHEGYL SEGRDEIVQN SRLARAALDA GLDAFIMNKM FTPRKWSAPL 

      1030       1040       1050       1060       1070       1080 
ISEKISLTPK QRTMSTKVLA DVVEALIGAS YIDGGFAAAH ACIHRFLPEV NLENIDRTTA 

      1090       1100       1110       1120       1130       1140 
PMPKDGVTNH TLNDDHLMAH IGYTFTNKSL LVESLTHPSC QFDTTTQSYQ RLEFLGDAVL 

      1150       1160       1170       1180       1190       1200 
DMAIMSTLLS HPREIPQGLM TKIKHAVVNA NLLAFFCMEF ALTEKRTNVQ VTPTGTVTLN 

      1210       1220       1230       1240       1250       1260 
PSTEHIELWR FMRYQGAHLQ TARDLALSRH SSLRGSIIHG LKHSPSYPWK SLSQLNADKF 

      1270       1280       1290       1300       1310       1320 
FSDIIESILG AIFIDSHGNL AECEKFLERL GLLRYLRRIL KDEVDVMHPR NIAQQMAKGE 

      1330       1340       1350       1360       1370       1380 
IRFEVLRVPN EGGGGGEDDG ATYRCTVKMA GVDGVAVVVE GCLTSEEAEI TAAERAVEIL 


VGRGCSL 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM270320 Genomic DNA. Translation: CAK46537.1. Sequence problems.

3D structure databases

ProteinModelPortalA2R345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5061.CADANGAP00011017.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG1111.
HOGENOMHOG000048683.
OrthoDBEOG76QFRP.

Family and domain databases

Gene3D1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProIPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDCL21_ASPNC
AccessionPrimary (citable) accession number: A2R345
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: April 16, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families