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Protein

Dicer-like protein 2-1

Gene

dcl2-1

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1133Magnesium or manganeseBy similarity1
Sitei1259Important for activityBy similarity1
Metal bindingi1263Magnesium or manganeseBy similarity1
Metal bindingi1266Magnesium or manganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntiviral protein, Helicase, Hydrolase, RNA-binding
Biological processAntiviral defense
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 2-1
Including the following 2 domains:
Endoribonuclease dcl2-1 (EC:3.1.26.-)
ATP-dependent helicase dcl2-1 (EC:3.6.4.-)
Gene namesi
Name:dcl2-1
ORF Names:An14g03000
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 1R

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003067871 – 1387Dicer-like protein 2-1Add BLAST1387

Proteomic databases

PaxDbiA2R345
PRIDEiA2R345

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00011017

Structurei

3D structure databases

ProteinModelPortaliA2R345
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 205Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST180
Domaini370 – 535Helicase C-terminalPROSITE-ProRule annotationAdd BLAST166
Domaini565 – 659Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST95
Domaini915 – 1055RNase III 1PROSITE-ProRule annotationAdd BLAST141
Domaini1094 – 1277RNase III 2PROSITE-ProRule annotationAdd BLAST184

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 149DEAH box4

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000048683
OrthoDBiEOG092C02VR

Family and domain databases

CDDicd00079 HELICc, 1 hit
cd00593 RIBOc, 2 hits
Gene3Di1.10.1520.10, 2 hits
3.30.160.380, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR038248 Dicer_dimer_sf
IPR005034 Dicer_dimerisation_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF03368 Dicer_dimer, 1 hit
PF00271 Helicase_C, 1 hit
PF00636 Ribonuclease_3, 2 hits
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00535 RIBOc, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
SSF69065 SSF69065, 3 hits
PROSITEiView protein in PROSITE
PS51327 DICER_DSRBF, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 2 hits

Sequencei

Sequence statusi: Complete.

A2R345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVAATVLPA GEDAPAYRPR SYQVEMFEAS LKENIIVTMG TGSGKTHIAL
60 70 80 90 100
LRIIKELESN PHKLIWFLTP TVALCLQQFK FLSDNIPAVR ARTLTSLDKV
110 120 130 140 150
ELWTEQPVWD AILKEMQVVV STHAVLADAM SHGFVKITQL GLMIFDEAHH
160 170 180 190 200
CMRRHPANKI MQDFYHPALE RHGAEAVPKI LGLTASPVVR SNRQELLKIE
210 220 230 240 250
SNLDAVCKTP RTHRSELMTH THRPHLQQIL FTPVLLDDLQ VGSKTLKALV
260 270 280 290 300
SAWTSLRLED DPYIKKLRKS PLDGRALQKV LESGKTYCND QLKRFATRSL
310 320 330 340 350
HIFEELGEWA ADYFIHASIE QLKARAGNSA DTMGWTDEEK AYLLDIVSKL
360 370 380 390 400
PIPNIDLTHS DPDRIPISSK FRSLLEFLDT KGEPNFSGLI FAKQRATVSV
410 420 430 440 450
MEKLLSIHPV TKHRFRCASF VGWSGGGSKD VLGELLDARM QRDTLSEFRT
460 470 480 490 500
GQKNLIIATD VLEEGIDISA CSVVVCFDKP PNLKSFVQRR GRARHRQSTY
510 520 530 540 550
AIMFATDDES SALSKWEDLE QAMIEAYEDD ERRLREAWAL EAINEEVVER
560 570 580 590 600
LEVQSTGAVL TADTAVAHLN HFCAVLPRQP YASNEPEFSY EKDDADLLRG
610 620 630 640 650
TVTLPSCVHP GVRRIQGQRW WQTERAARKE AAFQAYKRLY EFGLLSDHLL
660 670 680 690 700
PFKRNLELKE TDLTNLPALV EVSEQYDPWV DWACSWSSPD VHQTRIAIKH
710 720 730 740 750
NGDSRMCIRL TSPTSLPPVE PMTLFWDSET IYTLDFDKPK RMKEIAAESI
760 770 780 790 800
ENMRLATALY LQAASSRQMR PEQDFVTLFG PDLTDLELAE WLNKHAGDEP
810 820 830 840 850
ALEVYSRKDF PTVMGIVRDR SRYNEPMLFK RWVVSGQDDT PIVELECDAV
860 870 880 890 900
PKRRNLLHRQ TLAAKQPDSE TPAISSKIRL ILAENCTIDK LPYAETIFGR
910 920 930 940 950
FISVILDRLE ATLVATRLCE TILRDLEFSS IRHIITAITA PSAQSLTNYQ
960 970 980 990 1000
RYEFFGDSVL KFTVSCQLFF QHPNWHEGYL SEGRDEIVQN SRLARAALDA
1010 1020 1030 1040 1050
GLDAFIMNKM FTPRKWSAPL ISEKISLTPK QRTMSTKVLA DVVEALIGAS
1060 1070 1080 1090 1100
YIDGGFAAAH ACIHRFLPEV NLENIDRTTA PMPKDGVTNH TLNDDHLMAH
1110 1120 1130 1140 1150
IGYTFTNKSL LVESLTHPSC QFDTTTQSYQ RLEFLGDAVL DMAIMSTLLS
1160 1170 1180 1190 1200
HPREIPQGLM TKIKHAVVNA NLLAFFCMEF ALTEKRTNVQ VTPTGTVTLN
1210 1220 1230 1240 1250
PSTEHIELWR FMRYQGAHLQ TARDLALSRH SSLRGSIIHG LKHSPSYPWK
1260 1270 1280 1290 1300
SLSQLNADKF FSDIIESILG AIFIDSHGNL AECEKFLERL GLLRYLRRIL
1310 1320 1330 1340 1350
KDEVDVMHPR NIAQQMAKGE IRFEVLRVPN EGGGGGEDDG ATYRCTVKMA
1360 1370 1380
GVDGVAVVVE GCLTSEEAEI TAAERAVEIL VGRGCSL
Length:1,387
Mass (Da):156,456
Last modified:October 2, 2007 - v2
Checksum:i68B17C1A26553265
GO

Sequence cautioni

The sequence CAK46537 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270320 Genomic DNA Translation: CAK46537.1 Sequence problems.

Genome annotation databases

EnsemblFungiiCAK46537; CAK46537; An14g03000

Similar proteinsi

Entry informationi

Entry nameiDCL21_ASPNC
AccessioniPrimary (citable) accession number: A2R345
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: May 23, 2018
This is version 75 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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