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A2R345

- DCL21_ASPNC

UniProt

A2R345 - DCL21_ASPNC

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Protein

Dicer-like protein 2-1

Gene

dcl2-1

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity

Cofactori

Magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1133 – 11331Magnesium or manganeseBy similarity
Sitei1259 – 12591Important for activityBy similarity
Metal bindingi1263 – 12631Magnesium or manganeseBy similarity
Metal bindingi1266 – 12661Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. helicase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. ribonuclease III activity Source: InterPro
  5. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. regulation of defense response to virus Source: UniProtKB-KW
  3. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Helicase, Hydrolase

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 2-1
Including the following 2 domains:
Endoribonuclease dcl2-1 (EC:3.1.26.-)
ATP-dependent helicase dcl2-1 (EC:3.6.4.-)
Gene namesi
Name:dcl2-1
ORF Names:An14g03000
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 1R

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13871387Dicer-like protein 2-1PRO_0000306787Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00011017.

Structurei

3D structure databases

ProteinModelPortaliA2R345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 205180Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini370 – 535166Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini565 – 65995Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
BLAST
Domaini915 – 1055141RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1094 – 1277184RNase III 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi146 – 1494DEAH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1111.
HOGENOMiHOG000048683.
OrthoDBiEOG76QFRP.

Family and domain databases

Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2R345-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVAATVLPA GEDAPAYRPR SYQVEMFEAS LKENIIVTMG TGSGKTHIAL
60 70 80 90 100
LRIIKELESN PHKLIWFLTP TVALCLQQFK FLSDNIPAVR ARTLTSLDKV
110 120 130 140 150
ELWTEQPVWD AILKEMQVVV STHAVLADAM SHGFVKITQL GLMIFDEAHH
160 170 180 190 200
CMRRHPANKI MQDFYHPALE RHGAEAVPKI LGLTASPVVR SNRQELLKIE
210 220 230 240 250
SNLDAVCKTP RTHRSELMTH THRPHLQQIL FTPVLLDDLQ VGSKTLKALV
260 270 280 290 300
SAWTSLRLED DPYIKKLRKS PLDGRALQKV LESGKTYCND QLKRFATRSL
310 320 330 340 350
HIFEELGEWA ADYFIHASIE QLKARAGNSA DTMGWTDEEK AYLLDIVSKL
360 370 380 390 400
PIPNIDLTHS DPDRIPISSK FRSLLEFLDT KGEPNFSGLI FAKQRATVSV
410 420 430 440 450
MEKLLSIHPV TKHRFRCASF VGWSGGGSKD VLGELLDARM QRDTLSEFRT
460 470 480 490 500
GQKNLIIATD VLEEGIDISA CSVVVCFDKP PNLKSFVQRR GRARHRQSTY
510 520 530 540 550
AIMFATDDES SALSKWEDLE QAMIEAYEDD ERRLREAWAL EAINEEVVER
560 570 580 590 600
LEVQSTGAVL TADTAVAHLN HFCAVLPRQP YASNEPEFSY EKDDADLLRG
610 620 630 640 650
TVTLPSCVHP GVRRIQGQRW WQTERAARKE AAFQAYKRLY EFGLLSDHLL
660 670 680 690 700
PFKRNLELKE TDLTNLPALV EVSEQYDPWV DWACSWSSPD VHQTRIAIKH
710 720 730 740 750
NGDSRMCIRL TSPTSLPPVE PMTLFWDSET IYTLDFDKPK RMKEIAAESI
760 770 780 790 800
ENMRLATALY LQAASSRQMR PEQDFVTLFG PDLTDLELAE WLNKHAGDEP
810 820 830 840 850
ALEVYSRKDF PTVMGIVRDR SRYNEPMLFK RWVVSGQDDT PIVELECDAV
860 870 880 890 900
PKRRNLLHRQ TLAAKQPDSE TPAISSKIRL ILAENCTIDK LPYAETIFGR
910 920 930 940 950
FISVILDRLE ATLVATRLCE TILRDLEFSS IRHIITAITA PSAQSLTNYQ
960 970 980 990 1000
RYEFFGDSVL KFTVSCQLFF QHPNWHEGYL SEGRDEIVQN SRLARAALDA
1010 1020 1030 1040 1050
GLDAFIMNKM FTPRKWSAPL ISEKISLTPK QRTMSTKVLA DVVEALIGAS
1060 1070 1080 1090 1100
YIDGGFAAAH ACIHRFLPEV NLENIDRTTA PMPKDGVTNH TLNDDHLMAH
1110 1120 1130 1140 1150
IGYTFTNKSL LVESLTHPSC QFDTTTQSYQ RLEFLGDAVL DMAIMSTLLS
1160 1170 1180 1190 1200
HPREIPQGLM TKIKHAVVNA NLLAFFCMEF ALTEKRTNVQ VTPTGTVTLN
1210 1220 1230 1240 1250
PSTEHIELWR FMRYQGAHLQ TARDLALSRH SSLRGSIIHG LKHSPSYPWK
1260 1270 1280 1290 1300
SLSQLNADKF FSDIIESILG AIFIDSHGNL AECEKFLERL GLLRYLRRIL
1310 1320 1330 1340 1350
KDEVDVMHPR NIAQQMAKGE IRFEVLRVPN EGGGGGEDDG ATYRCTVKMA
1360 1370 1380
GVDGVAVVVE GCLTSEEAEI TAAERAVEIL VGRGCSL
Length:1,387
Mass (Da):156,456
Last modified:October 2, 2007 - v2
Checksum:i68B17C1A26553265
GO

Sequence cautioni

The sequence CAK46537.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270320 Genomic DNA. Translation: CAK46537.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270320 Genomic DNA. Translation: CAK46537.1 . Sequence problems.

3D structure databases

ProteinModelPortali A2R345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00011017.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG1111.
HOGENOMi HOG000048683.
OrthoDBi EOG76QFRP.

Family and domain databases

Gene3Di 1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEi PS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiDCL21_ASPNC
AccessioniPrimary (citable) accession number: A2R345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3