SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A2R345

- DCL21_ASPNC

UniProt

A2R345 - DCL21_ASPNC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Dicer-like protein 2-1
Gene
dcl2-1, An14g03000
Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.

Cofactori

Magnesium or manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1133 – 11331Magnesium or manganese By similarity
Sitei1259 – 12591Important for activity By similarity
Metal bindingi1263 – 12631Magnesium or manganese By similarity
Metal bindingi1266 – 12661Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. helicase activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. ribonuclease III activity Source: InterPro

GO - Biological processi

  1. RNA processing Source: InterPro
  2. defense response to virus Source: UniProtKB-KW
  3. regulation of defense response to virus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Helicase, Hydrolase

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 2-1
Including the following 2 domains:
Endoribonuclease dcl2-1 (EC:3.1.26.-)
ATP-dependent helicase dcl2-1 (EC:3.6.4.-)
Gene namesi
Name:dcl2-1
ORF Names:An14g03000
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 1R

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13871387Dicer-like protein 2-1
PRO_0000306787Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00011017.

Structurei

3D structure databases

ProteinModelPortaliA2R345.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 205180Helicase ATP-binding
Add
BLAST
Domaini370 – 535166Helicase C-terminal
Add
BLAST
Domaini565 – 65995Dicer dsRNA-binding fold
Add
BLAST
Domaini915 – 1055141RNase III 1
Add
BLAST
Domaini1094 – 1277184RNase III 2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi146 – 1494DEAH box

Sequence similaritiesi

Contains 2 RNase III domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1111.
HOGENOMiHOG000048683.
OrthoDBiEOG76QFRP.

Family and domain databases

Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2R345-1 [UniParc]FASTAAdd to Basket

« Hide

MTVAATVLPA GEDAPAYRPR SYQVEMFEAS LKENIIVTMG TGSGKTHIAL     50
LRIIKELESN PHKLIWFLTP TVALCLQQFK FLSDNIPAVR ARTLTSLDKV 100
ELWTEQPVWD AILKEMQVVV STHAVLADAM SHGFVKITQL GLMIFDEAHH 150
CMRRHPANKI MQDFYHPALE RHGAEAVPKI LGLTASPVVR SNRQELLKIE 200
SNLDAVCKTP RTHRSELMTH THRPHLQQIL FTPVLLDDLQ VGSKTLKALV 250
SAWTSLRLED DPYIKKLRKS PLDGRALQKV LESGKTYCND QLKRFATRSL 300
HIFEELGEWA ADYFIHASIE QLKARAGNSA DTMGWTDEEK AYLLDIVSKL 350
PIPNIDLTHS DPDRIPISSK FRSLLEFLDT KGEPNFSGLI FAKQRATVSV 400
MEKLLSIHPV TKHRFRCASF VGWSGGGSKD VLGELLDARM QRDTLSEFRT 450
GQKNLIIATD VLEEGIDISA CSVVVCFDKP PNLKSFVQRR GRARHRQSTY 500
AIMFATDDES SALSKWEDLE QAMIEAYEDD ERRLREAWAL EAINEEVVER 550
LEVQSTGAVL TADTAVAHLN HFCAVLPRQP YASNEPEFSY EKDDADLLRG 600
TVTLPSCVHP GVRRIQGQRW WQTERAARKE AAFQAYKRLY EFGLLSDHLL 650
PFKRNLELKE TDLTNLPALV EVSEQYDPWV DWACSWSSPD VHQTRIAIKH 700
NGDSRMCIRL TSPTSLPPVE PMTLFWDSET IYTLDFDKPK RMKEIAAESI 750
ENMRLATALY LQAASSRQMR PEQDFVTLFG PDLTDLELAE WLNKHAGDEP 800
ALEVYSRKDF PTVMGIVRDR SRYNEPMLFK RWVVSGQDDT PIVELECDAV 850
PKRRNLLHRQ TLAAKQPDSE TPAISSKIRL ILAENCTIDK LPYAETIFGR 900
FISVILDRLE ATLVATRLCE TILRDLEFSS IRHIITAITA PSAQSLTNYQ 950
RYEFFGDSVL KFTVSCQLFF QHPNWHEGYL SEGRDEIVQN SRLARAALDA 1000
GLDAFIMNKM FTPRKWSAPL ISEKISLTPK QRTMSTKVLA DVVEALIGAS 1050
YIDGGFAAAH ACIHRFLPEV NLENIDRTTA PMPKDGVTNH TLNDDHLMAH 1100
IGYTFTNKSL LVESLTHPSC QFDTTTQSYQ RLEFLGDAVL DMAIMSTLLS 1150
HPREIPQGLM TKIKHAVVNA NLLAFFCMEF ALTEKRTNVQ VTPTGTVTLN 1200
PSTEHIELWR FMRYQGAHLQ TARDLALSRH SSLRGSIIHG LKHSPSYPWK 1250
SLSQLNADKF FSDIIESILG AIFIDSHGNL AECEKFLERL GLLRYLRRIL 1300
KDEVDVMHPR NIAQQMAKGE IRFEVLRVPN EGGGGGEDDG ATYRCTVKMA 1350
GVDGVAVVVE GCLTSEEAEI TAAERAVEIL VGRGCSL 1387
Length:1,387
Mass (Da):156,456
Last modified:October 2, 2007 - v2
Checksum:i68B17C1A26553265
GO

Sequence cautioni

The sequence CAK46537.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270320 Genomic DNA. Translation: CAK46537.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270320 Genomic DNA. Translation: CAK46537.1 . Sequence problems.

3D structure databases

ProteinModelPortali A2R345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00011017.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG1111.
HOGENOMi HOG000048683.
OrthoDBi EOG76QFRP.

Family and domain databases

Gene3Di 1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProi IPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEi PS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiDCL21_ASPNC
AccessioniPrimary (citable) accession number: A2R345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi