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A2R345

- DCL21_ASPNC

UniProt

A2R345 - DCL21_ASPNC

Protein

Dicer-like protein 2-1

Gene

dcl2-1

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.By similarity

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1133 – 11331Magnesium or manganeseBy similarity
    Sitei1259 – 12591Important for activityBy similarity
    Metal bindingi1263 – 12631Magnesium or manganeseBy similarity
    Metal bindingi1266 – 12661Magnesium or manganeseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 468ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. ribonuclease III activity Source: InterPro
    5. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. regulation of defense response to virus Source: UniProtKB-KW
    3. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Antiviral protein, Helicase, Hydrolase

    Keywords - Biological processi

    Antiviral defense

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dicer-like protein 2-1
    Including the following 2 domains:
    Endoribonuclease dcl2-1 (EC:3.1.26.-)
    ATP-dependent helicase dcl2-1 (EC:3.6.4.-)
    Gene namesi
    Name:dcl2-1
    ORF Names:An14g03000
    OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
    Taxonomic identifieri425011 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006706: Chromosome 1R

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13871387Dicer-like protein 2-1PRO_0000306787Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5061.CADANGAP00011017.

    Structurei

    3D structure databases

    ProteinModelPortaliA2R345.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 205180Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini370 – 535166Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini565 – 65995Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
    BLAST
    Domaini915 – 1055141RNase III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1094 – 1277184RNase III 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi146 – 1494DEAH box

    Sequence similaritiesi

    Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
    Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 2 RNase III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1111.
    HOGENOMiHOG000048683.
    OrthoDBiEOG76QFRP.

    Family and domain databases

    Gene3Di1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProiIPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEiPS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A2R345-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVAATVLPA GEDAPAYRPR SYQVEMFEAS LKENIIVTMG TGSGKTHIAL     50
    LRIIKELESN PHKLIWFLTP TVALCLQQFK FLSDNIPAVR ARTLTSLDKV 100
    ELWTEQPVWD AILKEMQVVV STHAVLADAM SHGFVKITQL GLMIFDEAHH 150
    CMRRHPANKI MQDFYHPALE RHGAEAVPKI LGLTASPVVR SNRQELLKIE 200
    SNLDAVCKTP RTHRSELMTH THRPHLQQIL FTPVLLDDLQ VGSKTLKALV 250
    SAWTSLRLED DPYIKKLRKS PLDGRALQKV LESGKTYCND QLKRFATRSL 300
    HIFEELGEWA ADYFIHASIE QLKARAGNSA DTMGWTDEEK AYLLDIVSKL 350
    PIPNIDLTHS DPDRIPISSK FRSLLEFLDT KGEPNFSGLI FAKQRATVSV 400
    MEKLLSIHPV TKHRFRCASF VGWSGGGSKD VLGELLDARM QRDTLSEFRT 450
    GQKNLIIATD VLEEGIDISA CSVVVCFDKP PNLKSFVQRR GRARHRQSTY 500
    AIMFATDDES SALSKWEDLE QAMIEAYEDD ERRLREAWAL EAINEEVVER 550
    LEVQSTGAVL TADTAVAHLN HFCAVLPRQP YASNEPEFSY EKDDADLLRG 600
    TVTLPSCVHP GVRRIQGQRW WQTERAARKE AAFQAYKRLY EFGLLSDHLL 650
    PFKRNLELKE TDLTNLPALV EVSEQYDPWV DWACSWSSPD VHQTRIAIKH 700
    NGDSRMCIRL TSPTSLPPVE PMTLFWDSET IYTLDFDKPK RMKEIAAESI 750
    ENMRLATALY LQAASSRQMR PEQDFVTLFG PDLTDLELAE WLNKHAGDEP 800
    ALEVYSRKDF PTVMGIVRDR SRYNEPMLFK RWVVSGQDDT PIVELECDAV 850
    PKRRNLLHRQ TLAAKQPDSE TPAISSKIRL ILAENCTIDK LPYAETIFGR 900
    FISVILDRLE ATLVATRLCE TILRDLEFSS IRHIITAITA PSAQSLTNYQ 950
    RYEFFGDSVL KFTVSCQLFF QHPNWHEGYL SEGRDEIVQN SRLARAALDA 1000
    GLDAFIMNKM FTPRKWSAPL ISEKISLTPK QRTMSTKVLA DVVEALIGAS 1050
    YIDGGFAAAH ACIHRFLPEV NLENIDRTTA PMPKDGVTNH TLNDDHLMAH 1100
    IGYTFTNKSL LVESLTHPSC QFDTTTQSYQ RLEFLGDAVL DMAIMSTLLS 1150
    HPREIPQGLM TKIKHAVVNA NLLAFFCMEF ALTEKRTNVQ VTPTGTVTLN 1200
    PSTEHIELWR FMRYQGAHLQ TARDLALSRH SSLRGSIIHG LKHSPSYPWK 1250
    SLSQLNADKF FSDIIESILG AIFIDSHGNL AECEKFLERL GLLRYLRRIL 1300
    KDEVDVMHPR NIAQQMAKGE IRFEVLRVPN EGGGGGEDDG ATYRCTVKMA 1350
    GVDGVAVVVE GCLTSEEAEI TAAERAVEIL VGRGCSL 1387
    Length:1,387
    Mass (Da):156,456
    Last modified:October 2, 2007 - v2
    Checksum:i68B17C1A26553265
    GO

    Sequence cautioni

    The sequence CAK46537.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM270320 Genomic DNA. Translation: CAK46537.1. Sequence problems.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM270320 Genomic DNA. Translation: CAK46537.1 . Sequence problems.

    3D structure databases

    ProteinModelPortali A2R345.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5061.CADANGAP00011017.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG1111.
    HOGENOMi HOG000048683.
    OrthoDBi EOG76QFRP.

    Family and domain databases

    Gene3Di 1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProi IPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEi PS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
      Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
      , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
      Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CBS 513.88 / FGSC A1513.

    Entry informationi

    Entry nameiDCL21_ASPNC
    AccessioniPrimary (citable) accession number: A2R345
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3