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Protein

Probable alpha-galactosidase D

Gene

aglD

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551NucleophileBy similarity
Active sitei222 – 2221Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-galactosidase D (EC:3.2.1.22)
Alternative name(s):
Melibiase D
Gene namesi
Name:aglD
ORF Names:An14g01800
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 1R

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 660640Probable alpha-galactosidase DPRO_5000220909Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence analysis
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence analysis
Disulfide bondi124 ↔ 157By similarity
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence analysis
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence analysis
Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence analysis
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence analysis
Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence analysis
Glycosylationi506 – 5061N-linked (GlcNAc...)Sequence analysis
Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence analysis
Glycosylationi584 – 5841N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni200 – 2045Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000161224.
KOiK07407.
OrthoDBiEOG7CNZQQ.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2R2S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLHFILYAA LSSVVTSVSL QPRLQDGLAL TPQMGWNTYN HYSCSPNETI
60 70 80 90 100
VQSNAQALVD LGLSSLGYRY VTTDCGWTVA DRLPDGSLTW NDTLFPQGFP
110 120 130 140 150
AMGDFLHDLG LLFGVYQDSG ILLCGSPPNE TGSLYHEEQD ARTFASWNVD
160 170 180 190 200
SLKYDNCYSD AATGYPNVNY APSTSPEPRF ANMSHALLQQ NRTILFQICE
210 220 230 240 250
WGISFPANWA PALGHSWRIG NDIIPDWRTI FRIINQAAPQ TDVAGPGQWP
260 270 280 290 300
DLDMLEVGNN IFSLPEEQTH FSLWAILKSP LIIGAALKDE LTAINDASLA
310 320 330 340 350
VLKQKDVIAF NQDALGKSAS LRRRWTEEGY EVWSGPLSNG RTVAAVINWH
360 370 380 390 400
NESKDLTLDL PDVGLQHAGT VKNIWDGTTA RDVLTSYTAT VAGHGTMLLE
410 420 430 440 450
LQNTTAVGVY PRGVFGVSSG QTTTFQNIYA VTTSAKYIIS VYFSQPASST
460 470 480 490 500
ETITIGSNAN QSMISAQVPA SSTLVSAEIP LTAGSSNTIT INTSIPIDAI
510 520 530 540 550
HVTPPNGTYY PCTNFTLAGS TTLTTCGSGY CQPVGSKVGY ISPSGTAKAT
560 570 580 590 600
ISATTSGSKY LEIDWINNDI AFDSSWGWGS NSRNLTVTVN SEDPVRIEVP
610 620 630 640 650
LSGRHSELFG PGLGWWDTST LGLLTSGWKE GLNEVVVGNV GGDEGFQSYG
660
ADFVGIRVLD
Length:660
Mass (Da):71,228
Last modified:June 15, 2010 - v2
Checksum:iA92D587C27009651
GO

Sequence cautioni

The sequence CAK46492.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270315 Genomic DNA. Translation: CAK46492.1. Sequence problems.
RefSeqiXP_001400806.2. XM_001400769.2.

Genome annotation databases

GeneIDi4987036.
KEGGiang:ANI_1_276124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270315 Genomic DNA. Translation: CAK46492.1. Sequence problems.
RefSeqiXP_001400806.2. XM_001400769.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4987036.
KEGGiang:ANI_1_276124.

Phylogenomic databases

HOGENOMiHOG000161224.
KOiK07407.
OrthoDBiEOG7CNZQQ.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiAGALD_ASPNC
AccessioniPrimary (citable) accession number: A2R2S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: May 11, 2016
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.