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A2QYN2 (MANBB_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-mannosidase B

EC=3.2.1.25
Alternative name(s):
Mannanase B
Short name=Mannase B
Gene names
Name:mndB
ORF Names:An12g01850
OrganismAspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]
Taxonomic identifier425011 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length844 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Prefers manobiose over mannotriose and has no activity against polymeric mannan. Is also severly restricetd by galactosyl substitutions at the +1 subsite By similarity.

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Pathway

Glycan metabolism; N-glycan degradation.

Miscellaneous

In contrast to clade A beta-mannosidases, which are likely secreted, clade B proteins appear to be intracellular By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family. Beta-mannosidase B subfamily.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmannan catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 844844Beta-mannosidase B
PRO_0000394655

Sites

Active site4321Proton donor By similarity

Amino acid modifications

Glycosylation7231N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
A2QYN2 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: D24AE1CB82B9CEA0

FASTA84497,117
        10         20         30         40         50         60 
MAAFAQYTLS TGWYFKDRDE LASEAWMPVP VVPSVVHQDL QANGKLKNPY VGFNELDARW 

        70         80         90        100        110        120 
VNEKSWTYRK ILQKPTVLAG SRIVLAFDGL DTFAKVKLDN NIILESSNMF QAYRVDVTKA 

       130        140        150        160        170        180 
LDCGDEHVLE IEFDCAMLRA QELRKQDPNH NWASFNGDPA RMSVRKAQYH WGWDWGPVLM 

       190        200        210        220        230        240 
TAGIWRAVRL EVYSTRLADL WTEINLDPTH KTASISAFTE LESVDLDPSY KVKFTITLHG 

       250        260        270        280        290        300 
KQIAQAEATP QEGRAKVEFN IDQPCLWWPH GYGDSTLYEV SASLNADQLE LHRVTKKIGI 

       310        320        330        340        350        360 
RTAEVVQRPD KHGKSFFFRI NGVDIFCGGS CWIPADNLLP NISPQRYRKW IQLMVAGRQA 

       370        380        390        400        410        420 
MIRVWGGGCY EDDSFYEACD ELGVLVWQDF MFGCGNYPTW PELLKSIEQE AIYNVRRLRH 

       430        440        450        460        470        480 
HPSIVVYVGN NEDYQVQEQA GLEYNYEDKN PENWLKTNFP ARYIYEELLP SVVERCSPKT 

       490        500        510        520        530        540 
FYHPGSPWGD GKITSDPTVG DMHQWNVWHG TQEKYQIFDT LGGRFNSEFG MEAFPHLSTI 

       550        560        570        580        590        600 
EYFVENEKDK YPQSHVLDFH NKADGHERRI ATYLVENLRT ATDLETYIYL TQVVQAETMM 

       610        620        630        640        650        660 
FGYRGWRRQW GDERHCGGAL LWQLNDCWPT ISWAIVDYFL RPKPAYYAVA RVLNPVAVGV 

       670        680        690        700        710        720 
RREHHDWSIT HAQPPKTSKY ELWVVSSLQK PASGKVELRF LSVDTGREIR ERIVRENVDI 

       730        740        750        760        770        780 
VPNGTTNLIT DGLIDHTVDA EPHVLAARLW VDGEIVARDV DWPQPFKYLD FADRGLEVKR 

       790        800        810        820        830        840 
VSEASDQQVF QISTEKPVKC LVFEERDGVK FSDSAMDIVP GDVQTVKVTG LRADEKLKYK 


FLGQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM270263 Genomic DNA. Translation: CAK48467.1.
RefSeqXP_001395271.1. XM_001395234.2.

3D structure databases

ProteinModelPortalA2QYN2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5061.CADANGAP00009453.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANGAT00009634; CADANGAP00009453; CADANGAG00009634.
GeneID4985535.
KEGGang:ANI_1_250104.

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000186861.
KOK01192.
OrthoDBEOG7NSB9Q.

Enzyme and pathway databases

UniPathwayUPA00280.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMANBB_ASPNC
AccessionPrimary (citable) accession number: A2QYN2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 6, 2007
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries