Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A2QX45

- DCL22_ASPNC

UniProt

A2QX45 - DCL22_ASPNC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Dicer-like protein 2-2

Gene

dcl2-2

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 508ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. helicase activity Source: UniProtKB-KW
  3. ribonuclease III activity Source: InterPro
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. regulation of defense response to virus Source: UniProtKB-KW
  3. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Helicase, Hydrolase

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 2-2
Including the following 2 domains:
Endoribonuclease dcl2-2 (EC:3.1.26.-)
ATP-dependent helicase dcl2-2 (EC:3.6.4.-)
Gene namesi
Name:dcl2-2
ORF Names:An11g07590
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 7R

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13621362Dicer-like protein 2-2PRO_0000306788Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00008910.

Structurei

3D structure databases

ProteinModelPortaliA2QX45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 197168Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini343 – 505163Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini536 – 63095Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
BLAST
Domaini889 – 1033145RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1075 – 1258184RNase III 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi139 – 1424DEAH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1111.
HOGENOMiHOG000129941.
OrthoDBiEOG76QFRP.

Family and domain databases

Gene3Di1.10.1520.10. 3 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2QX45-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSSTDYHAD STPGTTSNME LRIREYQLEM LNESLKRNLI VVMPTGTGKT
60 70 80 90 100
QVAILRILAD IDKGNSDKFV WLLCPTVALS EQQYIQYATV FLPSGAEDKA
110 120 130 140 150
IWDNALSGIK IAVSTYQVLY DALSHGFVKL SQMSLLIFDE AHHCKKDHVA
160 170 180 190 200
NKIMQVHYHK QHQSGVQNLP KILGLTASPI LSDLSSLEIV ESNLGSICKT
210 220 230 240 250
PRQYYAQLLQ FTNRPLILPR LPTYTIPNCS VKAPILEKLC GILSSEDEVP
260 270 280 290 300
SSSKMKSKQL KHIRRFMQTS ESINQELGIW AATEYMRKSI MHFKESMRMG
310 320 330 340 350
AEKTNISNYG KDFAMEILTR LGKLQDCSPA IQPEEISPMC QCLLDELSKA
360 370 380 390 400
YREGFCGLVF VTQRATVLAL KWLIENHPLT SHLFTCGTFI GMSTTQYSKT
410 420 430 440 450
ELGNLHDIRN QTETLEKFRQ GSLNLIITTD ALEEGIDVPA CNTVLNFNCQ
460 470 480 490 500
LSLKSFIQRR GRARRENSQF IIIMEDESGP RYLKRLEMEE IELVQKLQNA
510 520 530 540 550
ERRQIPANEL DFDKYERISL SLDIDRTGAQ LIMREAVGYL YNFCSKLPAQ
560 570 580 590 600
LYVSNKPLFT YERNNYGRFR AVVKLPSNLD PSLQSFSSSR SWSRLKYARE
610 620 630 640 650
DAALQAYKAL YQAGLVNDYL VPTQVSDHLE GDIIFRSHYS IQNQLDPWQD
660 670 680 690 700
IALLWKLDSQ LYAHNLRIIR PEEDEIHLHM ILPTQLDTTI HIPLFIDYCT
710 720 730 740 750
TYTAILTPGH PITTDISLCQ QVTNLIFQSV YRDHCSRRNL DYAFLLVPEL
760 770 780 790 800
EETKLIEFLE RYSGSVSLTE LLNQEATPSA LGLLRSHTRP SRPLLVEPWV
810 820 830 840 850
AGECFLPDEL SISSFDAKVK YMTNRRNFLS HGNLAAGKST NCEARIGLEA
860 870 880 890 900
NVKSMSVRDF FVDKLPSMFA QVALFTPSIS HEVEVYMIAQ ILRQELSLRS
910 920 930 940 950
VTPWQRIDLL AIAIRPTSIE HRATFRLLAF IGDAFMKYLF AMQLFLHHHL
960 970 980 990 1000
WHEGLLSSLK QRNLSDAGLA HAIHQSGLGK FLISKHLNGK RWVPPLVSGI
1010 1020 1030 1040 1050
EPASNEARQR SIGAATLADM TKAVVGAAFT DGGLNQAAAC ASVMFPKLKS
1060 1070 1080 1090 1100
WNASSLHDGT YSKTRPENAV ASTAIVDMEE LLGYTFTDKS LAVESMTHPS
1110 1120 1130 1140 1150
CTGLVQTTSY RRLSFLGASV LEWIVVSYLH RHAQVMNPQR MQSLKSAFTN
1160 1170 1180 1190 1200
NTFLTFIAIT FHQVREQNHI DVDDEHNVHK NVTTCSIRLW DFLRLHSDAL
1210 1220 1230 1240 1250
STELSDFVQK SSEKADAIKH ELWEQRFYPW VRLRALGDMR VLSDIIQSIF
1260 1270 1280 1290 1300
GAVFIDSQAT LASCDALAEK LGIVPLLEHF ISHQITTDHP KDTLQAILPG
1310 1320 1330 1340 1350
RKVSYQICVD KVHPGTLRCS ALADSSEIAS VEGQMNDEVI KMQAAETAVR
1360
LLRKGFALSE TS
Length:1,362
Mass (Da):153,885
Last modified:October 2, 2007 - v2
Checksum:iD233E94793C21EB3
GO

Sequence cautioni

The sequence CAK40801.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270243 Genomic DNA. Translation: CAK40801.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270243 Genomic DNA. Translation: CAK40801.1 . Sequence problems.

3D structure databases

ProteinModelPortali A2QX45.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00008910.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG1111.
HOGENOMi HOG000129941.
OrthoDBi EOG76QFRP.

Family and domain databases

Gene3Di 1.10.1520.10. 3 hits.
3.40.50.300. 2 hits.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEi PS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiDCL22_ASPNC
AccessioniPrimary (citable) accession number: A2QX45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3