Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A2QS05

- NCPR_ASPNC

UniProt

A2QS05 - NCPR_ASPNC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis (By similarity).By similarity

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.By similarity

Cofactori

FAD.By similarity
FMN.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 765FMNBy similarityPROSITE-ProRule annotation
Nucleotide bindingi166 – 19732FMNBy similarityPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi317 – 32812FADBy similarityAdd
BLAST
Nucleotide bindingi447 – 45812FADBy similarityAdd
BLAST
Nucleotide bindingi547 – 56519NADPBy similarityAdd
BLAST
Nucleotide bindingi642 – 65817NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseBy similarity (EC:1.6.2.4)
Short name:
CPRBy similarity
Short name:
P450RBy similarity
Gene namesi
Name:cprA
ORF Names:An08g07840
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 8R

Subcellular locationi

Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity. Cell membrane By similarity; Single-pass membrane protein By similarity. Microsome By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695NADPH--cytochrome P450 reductasePRO_5000220324Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00006979.

Structurei

3D structure databases

ProteinModelPortaliA2QS05.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 3123HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 221156Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini277 – 538262FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Sequence Analysis
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000282027.
KOiK00327.
OrthoDBiEOG744TKC.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2QS05-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQLDTLDLV VLAVLLVGSV AYFTKGTYWA VAKDPYASTG PAMNGAAKAG
60 70 80 90 100
KTRNIIEKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD
110 120 130 140 150
LEEYDYENLD QFPEDKVAFF VLATYGEGEP TDNAVEFYQF FTGDDVAFES
160 170 180 190 200
GASADEKPLS KLKYVAFGLG NNTYEHYNAM VRQVDAAFQK LGAQRIGSAG
210 220 230 240 250
EGDDGAGTME EDFLAWKEPM WAALSESMDL QEREAVYEPV FCVTENESLS
260 270 280 290 300
PEDETVYLGE PTQSHLQGTP KGPYSAHNPF IAPIAESREL FTVKDRNCLH
310 320 330 340 350
MEISIAGSNL SYQTGDHIAV WPTNAGAEVD RFLQVFGLEG KRDSVINIKG
360 370 380 390 400
IDVTAKVPIP TPTTYDAAVR YYMEVCAPVS RQFVATLAAF APDEESKAEI
410 420 430 440 450
VRLGSDKDYF HEKVTNQCFN IAQALQSITS KPFSAVPFSL LIEGITKLQP
460 470 480 490 500
RYYSISSSSL VQKDKISITA VVESVRLPGA SHMVKGVTTN YLLALKQKQN
510 520 530 540 550
GDPSPDPHGL TYSITGPRNK YDGIHVPVHV RHSNFKLPSD PSRPIIMVGP
560 570 580 590 600
GTGVAPFRGF IQERAALAAK GEKVGPTVLF FGCRKSDEDF LYKDEWKTYQ
610 620 630 640 650
DQLGDNLKII TAFSREGPQK VYVQHRLREH SELVSDLLKQ KATFYVCGDA
660 670 680 690
ANMAREVNLV LGQIIAAQRG LPAEKGEEMV KHMRSSGSYQ EDVWS
Length:695
Mass (Da):76,589
Last modified:March 6, 2007 - v1
Checksum:i835324930A8A62F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270176 Genomic DNA. Translation: CAK45677.1.
RefSeqiXP_001392901.1. XM_001392864.2.

Genome annotation databases

EnsemblFungiiCADANGAT00007108; CADANGAP00006979; CADANGAG00007108.
GeneIDi4983103.
KEGGiang:ANI_1_1104074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270176 Genomic DNA. Translation: CAK45677.1 .
RefSeqi XP_001392901.1. XM_001392864.2.

3D structure databases

ProteinModelPortali A2QS05.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00006979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANGAT00007108 ; CADANGAP00006979 ; CADANGAG00007108 .
GeneIDi 4983103.
KEGGi ang:ANI_1_1104074.

Phylogenomic databases

HOGENOMi HOG000282027.
KOi K00327.
OrthoDBi EOG744TKC.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000208. P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiNCPR_ASPNC
AccessioniPrimary (citable) accession number: A2QS05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: March 6, 2007
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3