A2QL72 (AGALA_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-galactosidase A
EC=3.2.1.22

Alternative name(s):
Melibiase A

Gene names

Name:	aglA
ORF Names:	An06g00170

Organism

Aspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]

Taxonomic identifier

425011 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus niger

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Lectin
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 537	514	Probable alpha-galactosidase A		PRO_5000220032
Regions
	Domain	413 – 537	125	Ricin B-type lectin
Sites
	Active site	154	1	Nucleophile By similarity
	Active site	212	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	49	1	N-linked (GlcNAc...) Potential
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	203	1	N-linked (GlcNAc...) Potential
	Glycosylation	355	1	N-linked (GlcNAc...) Potential
	Glycosylation	436	1	N-linked (GlcNAc...) Potential
	Glycosylation	491	1	N-linked (GlcNAc...) Potential
	Disulfide bond	46 ↔ 78		By similarity
	Disulfide bond	126 ↔ 156		By similarity
	Disulfide bond	430 ↔ 444		By similarity
	Disulfide bond	469 ↔ 482		By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2QL72 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: F093FD8196C7F839	FASTA	537	59,213
	10 20 30 40 50 60 MNQGTKSILL AATLAAIPWQ VYGSIEQSSL LPIPPMGFNN WARFMCDLNE TLFTETADAM 70 80 90 100 110 120 AANGLRDAGY NRINLDDCWM AYQRSDNGSL QWNTTKFPHG LPWLAQYVKA KGFHFGIYED 130 140 150 160 170 180 SGNMTCGGYP GSYNHEEQDA NTFALWGIDY LKLDGCNVYA TQGRTLEEEY KQRYGHWHQV 190 200 210 220 230 240 LSKMQHPLIF SESAPAYFAG TDNNTDWYTV MDWVPIYGEL ARHSTDILVY SGAGSAWDSI 250 260 270 280 290 300 MNNYNYNTLL ARYQRPGYFN DPDFLIPDHP GLTADEKRSH FALWASFSAP LIISAYIPAL 310 320 330 340 350 360 SKDEIAFLTN EALIAVNQDP LAQQATFASR DNTLDILTRN LANGDRLLTV LNKGNTTVTR 370 380 390 400 410 420 DIPVQWLGLT ETDCTYTAED LWDGKTQKIS DHIKIELASH ATAVFRLGLP QGCSSVVPTG 430 440 450 460 470 480 LVFNTASGNC LTAASNSSVA FQSCNGETSQ IWQVTLSGVI RPVSQTTQCL AADGNSVKLQ 490 500 510 520 530 ACDSTDSDGQ NWTYAVTGNL KNAKTDGCLT EGSVQMKSCL YERDGQVFGL PSGVQLS

References

[1]

"Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88." Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M. , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H. Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CBS 513.88 / FGSC A1513.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM270108 Genomic DNA. Translation: CAK44933.1.
RefSeq	XP_001390845.1. XM_001390808.1.
3D structure databases
ProteinModelPortal	A2QL72.
ModBase	Search...
Protein-protein interaction databases
STRING	5061.CADANGAP00004923.
Protein family/group databases
CAZy	CBM13. Carbohydrate-Binding Module Family 13. GH27. Glycoside Hydrolase Family 27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANGAT00005022; CADANGAP00004923; CADANGAG00005022.
GeneID	4981013.
KEGG	ang:ANI_1_6054.
Phylogenomic databases
HOGENOM	HOG000161224.
KO	K07407.
OrthoDB	EOG48KVKH.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR017853. Glycoside_hydrolase_SF. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF02065. Melibiase. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27.
SMART	SM00458. RICIN. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF50370. RicinB_like. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGALA_ASPNC

Accession

Primary (citable) accession number: A2QL72

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program