Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable alpha-galactosidase A

Gene

aglA

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154NucleophileBy similarity1
Active sitei212Proton donorBy similarity1

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • galactosidase activity Source: AspGD
  • hydrolase activity, acting on glycosyl bonds Source: AspGD
  • raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandLectin

Enzyme and pathway databases

BRENDAi3.2.1.49 518

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GH27 Glycoside Hydrolase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-galactosidase A (EC:3.2.1.22)
Alternative name(s):
Melibiase A
Gene namesi
Name:aglA
ORF Names:An06g00170
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 8ER

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_500022003224 – 537Probable alpha-galactosidase AAdd BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi46 ↔ 78PROSITE-ProRule annotation
Glycosylationi49N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi87N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi93N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi123N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi126 ↔ 156PROSITE-ProRule annotation
Glycosylationi203N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi355N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi430 ↔ 444PROSITE-ProRule annotation
Glycosylationi436N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi469 ↔ 482PROSITE-ProRule annotation
Glycosylationi491N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiA2QL72
PRIDEiA2QL72

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00004923

Structurei

3D structure databases

ProteinModelPortaliA2QL72
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini413 – 537Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST125

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000161224
KOiK07407
OrthoDBiEOG092C1MJ6

Family and domain databases

CDDicd14792 GH27, 1 hit
cd00161 RICIN, 1 hit
Gene3Di2.60.40.1180, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR002241 Glyco_hydro_27
IPR000111 Glyco_hydro_27/36_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF16499 Melibiase_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
PRINTSiPR00740 GLHYDRLASE27
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00512 ALPHA_GALACTOSIDASE, 1 hit
PS50231 RICIN_B_LECTIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QL72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQGTKSILL AATLAAIPWQ VYGSIEQSSL LPIPPMGFNN WARFMCDLNE
60 70 80 90 100
TLFTETADAM AANGLRDAGY NRINLDDCWM AYQRSDNGSL QWNTTKFPHG
110 120 130 140 150
LPWLAQYVKA KGFHFGIYED SGNMTCGGYP GSYNHEEQDA NTFALWGIDY
160 170 180 190 200
LKLDGCNVYA TQGRTLEEEY KQRYGHWHQV LSKMQHPLIF SESAPAYFAG
210 220 230 240 250
TDNNTDWYTV MDWVPIYGEL ARHSTDILVY SGAGSAWDSI MNNYNYNTLL
260 270 280 290 300
ARYQRPGYFN DPDFLIPDHP GLTADEKRSH FALWASFSAP LIISAYIPAL
310 320 330 340 350
SKDEIAFLTN EALIAVNQDP LAQQATFASR DNTLDILTRN LANGDRLLTV
360 370 380 390 400
LNKGNTTVTR DIPVQWLGLT ETDCTYTAED LWDGKTQKIS DHIKIELASH
410 420 430 440 450
ATAVFRLGLP QGCSSVVPTG LVFNTASGNC LTAASNSSVA FQSCNGETSQ
460 470 480 490 500
IWQVTLSGVI RPVSQTTQCL AADGNSVKLQ ACDSTDSDGQ NWTYAVTGNL
510 520 530
KNAKTDGCLT EGSVQMKSCL YERDGQVFGL PSGVQLS
Length:537
Mass (Da):59,213
Last modified:March 6, 2007 - v1
Checksum:iF093FD8196C7F839
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270108 Genomic DNA Translation: CAK44933.1
RefSeqiXP_001390845.1, XM_001390808.1

Genome annotation databases

EnsemblFungiiCAK44933; CAK44933; An06g00170
GeneIDi4981013
KEGGiang:ANI_1_6054

Similar proteinsi

Entry informationi

Entry nameiAGALA_ASPNC
AccessioniPrimary (citable) accession number: A2QL72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: March 6, 2007
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health