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A2QL72

- AGALA_ASPNC

UniProt

A2QL72 - AGALA_ASPNC

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Protein

Probable alpha-galactosidase A

Gene

aglA

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541NucleophileBy similarity
Active sitei212 – 2121Proton donorBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. galactosidase activity Source: ASPGD
  3. hydrolase activity, acting on glycosyl bonds Source: ASPGD
  4. raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Lectin

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-galactosidase A (EC:3.2.1.22)
Alternative name(s):
Melibiase A
Gene namesi
Name:aglA
ORF Names:An06g00170
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 8ER

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 537514Probable alpha-galactosidase APRO_5000220032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 78PROSITE-ProRule annotation
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi126 ↔ 156PROSITE-ProRule annotation
Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi430 ↔ 444PROSITE-ProRule annotation
Glycosylationi436 – 4361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi469 ↔ 482PROSITE-ProRule annotation
Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00004923.

Structurei

3D structure databases

ProteinModelPortaliA2QL72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini413 – 537125Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated
Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000161224.
KOiK07407.
OrthoDBiEOG7SR4WK.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QL72 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQGTKSILL AATLAAIPWQ VYGSIEQSSL LPIPPMGFNN WARFMCDLNE
60 70 80 90 100
TLFTETADAM AANGLRDAGY NRINLDDCWM AYQRSDNGSL QWNTTKFPHG
110 120 130 140 150
LPWLAQYVKA KGFHFGIYED SGNMTCGGYP GSYNHEEQDA NTFALWGIDY
160 170 180 190 200
LKLDGCNVYA TQGRTLEEEY KQRYGHWHQV LSKMQHPLIF SESAPAYFAG
210 220 230 240 250
TDNNTDWYTV MDWVPIYGEL ARHSTDILVY SGAGSAWDSI MNNYNYNTLL
260 270 280 290 300
ARYQRPGYFN DPDFLIPDHP GLTADEKRSH FALWASFSAP LIISAYIPAL
310 320 330 340 350
SKDEIAFLTN EALIAVNQDP LAQQATFASR DNTLDILTRN LANGDRLLTV
360 370 380 390 400
LNKGNTTVTR DIPVQWLGLT ETDCTYTAED LWDGKTQKIS DHIKIELASH
410 420 430 440 450
ATAVFRLGLP QGCSSVVPTG LVFNTASGNC LTAASNSSVA FQSCNGETSQ
460 470 480 490 500
IWQVTLSGVI RPVSQTTQCL AADGNSVKLQ ACDSTDSDGQ NWTYAVTGNL
510 520 530
KNAKTDGCLT EGSVQMKSCL YERDGQVFGL PSGVQLS
Length:537
Mass (Da):59,213
Last modified:March 6, 2007 - v1
Checksum:iF093FD8196C7F839
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270108 Genomic DNA. Translation: CAK44933.1.
RefSeqiXP_001390845.1. XM_001390808.1.

Genome annotation databases

EnsemblFungiiCADANGAT00005022; CADANGAP00004923; CADANGAG00005022.
GeneIDi4981013.
KEGGiang:ANI_1_6054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM270108 Genomic DNA. Translation: CAK44933.1 .
RefSeqi XP_001390845.1. XM_001390808.1.

3D structure databases

ProteinModelPortali A2QL72.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00004923.

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GH27. Glycoside Hydrolase Family 27.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANGAT00005022 ; CADANGAP00004923 ; CADANGAG00005022 .
GeneIDi 4981013.
KEGGi ang:ANI_1_6054.

Phylogenomic databases

HOGENOMi HOG000161224.
KOi K07407.
OrthoDBi EOG7SR4WK.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF02065. Melibiase. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
PRINTSi PR00740. GLHYDRLASE27.
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiAGALA_ASPNC
AccessioniPrimary (citable) accession number: A2QL72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: March 6, 2007
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3