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Protein

Probable mannan endo-1,4-beta-mannosidase A

Gene

manA

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei206Proton donorBy similarity1
Active sitei314NucleophileBy similarity1

GO - Molecular functioni

  • mannan endo-1,4-beta-mannosidase activity Source: ASPGD

GO - Biological processi

  • mannan catabolic process Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannan endo-1,4-beta-mannosidase A (EC:3.2.1.78)
Alternative name(s):
Endo-beta-1,4-mannanase A
Gene namesi
Name:manA
Synonyms:man1
ORF Names:An05g01320
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 7L

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: ASPGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_500022000022 – 383Probable mannan endo-1,4-beta-mannosidase AAdd BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi17N-linked (GlcNAc...)Sequence analysis1
Glycosylationi194N-linked (GlcNAc...)Sequence analysis1
Glycosylationi263N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Structurei

Secondary structure

1383
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 44Combined sources3
Beta strandi47 – 50Combined sources4
Beta strandi53 – 55Combined sources3
Beta strandi57 – 61Combined sources5
Helixi65 – 67Combined sources3
Helixi71 – 84Combined sources14
Beta strandi88 – 92Combined sources5
Beta strandi96 – 99Combined sources4
Beta strandi108 – 112Combined sources5
Beta strandi115 – 118Combined sources4
Turni122 – 124Combined sources3
Helixi125 – 137Combined sources13
Beta strandi141 – 146Combined sources6
Beta strandi148 – 151Combined sources4
Helixi155 – 163Combined sources9
Helixi169 – 173Combined sources5
Helixi175 – 191Combined sources17
Turni192 – 194Combined sources3
Beta strandi198 – 203Combined sources6
Helixi215 – 231Combined sources17
Beta strandi235 – 238Combined sources4
Helixi254 – 256Combined sources3
Helixi264 – 268Combined sources5
Beta strandi275 – 280Combined sources6
Helixi282 – 285Combined sources4
Helixi291 – 306Combined sources16
Beta strandi310 – 315Combined sources6
Helixi321 – 334Combined sources14
Beta strandi338 – 344Combined sources7
Helixi367 – 372Combined sources6
Helixi374 – 381Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WH9X-ray1.57A/B39-383[»]
ProteinModelPortaliA2QKT4.
SMRiA2QKT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000169951.
KOiK19355.
OrthoDBiEOG092C2KMO.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QKT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSNALLTL ASLALANVST ALPKASPAPS TSSSAASTSF ASTSGLQFTI
60 70 80 90 100
DGETGYFAGT NSYWIGFLTD NADVDLVMGH LKSSGLKILR VWGFNDVTSQ
110 120 130 140 150
PSSGTVWYQL HQDGKSTINT GADGLQRLDY VVSSAEQHDI KLIINFVNYW
160 170 180 190 200
TDYGGMSAYV SAYGGSGETD FYTSDTMQSA YQTYIKTVVE RYSNSSAVFA
210 220 230 240 250
WELANEPRCP SCDTSVLYNW IEKTSKFIKG LDADRMVCIG DEGFGLNIDS
260 270 280 290 300
DGSYPYQFSE GLNFTMNLGI DTIDFGTLHL YPDSWGTSDD WGNGWITAHG
310 320 330 340 350
AACKAAGKPC LLEEYGVTSN HCSVEGSWQK TALSTTGVGA DLFWQYGDDL
360 370 380
STGKSPDDGN TIYYGTSDYQ CLVTDHVAAI GSA
Length:383
Mass (Da):41,247
Last modified:March 6, 2007 - v1
Checksum:i926472E9197CF491
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270105 Genomic DNA. Translation: CAK96471.1.
RefSeqiXP_001390707.1. XM_001390670.1.

Genome annotation databases

EnsemblFungiiCAK96471; CAK96471; An05g01320.
GeneIDi4980871.
KEGGiang:ANI_1_160044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270105 Genomic DNA. Translation: CAK96471.1.
RefSeqiXP_001390707.1. XM_001390670.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WH9X-ray1.57A/B39-383[»]
ProteinModelPortaliA2QKT4.
SMRiA2QKT4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAK96471; CAK96471; An05g01320.
GeneIDi4980871.
KEGGiang:ANI_1_160044.

Phylogenomic databases

HOGENOMiHOG000169951.
KOiK19355.
OrthoDBiEOG092C2KMO.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMANA_ASPNC
AccessioniPrimary (citable) accession number: A2QKT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 6, 2007
Last modified: November 30, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.