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A2QH21 (EGLC_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glucan endo-1,3-beta-glucosidase eglC

EC=3.2.1.39
Alternative name(s):
Endo-1,3-beta-glucanase eglC
Laminarinase eglC
Gene names
Name:eglC
ORF Names:An03g05290
OrganismAspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]
Taxonomic identifier425011 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase By similarity.

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor By similarity. Secretedcell wall By similarity. Note: Covalently-linked GPI-modified cell wall protein By similarity.

Post-translational modification

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Sequence caution

The sequence CAK49181.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 430412Probable glucan endo-1,3-beta-glucosidase eglC
PRO_5000219799
Propeptide431 – 46030Removed in mature form Potential
PRO_0000395144

Regions

Compositional bias309 – 445137Ser-rich

Sites

Active site1281Nucleophile By similarity
Active site2391Proton donor By similarity

Amino acid modifications

Lipidation4301GPI-anchor amidated serine Potential
Glycosylation1831N-linked (GlcNAc...) Potential
Glycosylation3121N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential
Glycosylation3731N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
A2QH21 [UniParc].

Last modified June 15, 2010. Version 2.
Checksum: F7B5114CE78833B2

FASTA46046,347
        10         20         30         40         50         60 
MQLAQLAAFA MTLATSEAAY QGFNYGNKFS DESSKFQADF EAEFKAAKNL VGTSGFTSAR 

        70         80         90        100        110        120 
LYTMIQAYST SDVIEAIPAA IAQDTSLLLG LWASGGGMDN EITALKTAIS QYGEELGKLV 

       130        140        150        160        170        180 
VGISVGSEDL YRNSVEGAEA DAGVGVNPDE LVEYIKEVRS VIAGTALADV SIGHVDTWDS 

       190        200        210        220        230        240 
WTNSSNSAVV EAVDWLGFDG YPFFQSSMAN SIDNAKTLFE ESVAKTKAVA GDKEVWITET 

       250        260        270        280        290        300 
GWPVSGDSQG DAVASIANAK TFWDEVGCPL FGNVNTWWYI LQDASPTTPN PSFGIVGSTL 

       310        320        330        340        350        360 
STTPLFDLSC KNSTTSSSSA VVSAAASSAA GSKAVGSSQA SSGAAAWATS ASGSAKPTFT 

       370        380        390        400        410        420 
VGRPGVNGTV FGNGTYPLRP SGSASARPSA GAISSGSGSS SSGSGSSGST GTSATSGQSS 

       430        440        450        460 
SSGSSAAAGS SSPAAFSGAS TLSGSLFGAV VAVFMTLAAL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM270057 Genomic DNA. Translation: CAK49181.1. Different initiation.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG5309.
HOGENOMHOG000179527.
OrthoDBEOG7TBCCK.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameEGLC_ASPNC
AccessionPrimary (citable) accession number: A2QH21
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: February 19, 2014
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries